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>
Transfection ½Ã¾à
>
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀԽþà
> VirusGEN¢ç AAV Transfection Kit
AAV »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç AAV Transfection Kit
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Mirus
MIR 6750
VirusGEN¢ç AAV Transfection Kit
1 Kit (1 L cell culture)
°¡°Ý¹®ÀÇ
05.02 ~ 06.30
Mirus
MIR 6770
VirusGEN¢ç SELECT AAV Transfection Kit
1 Kit (10 L cell culture)
°¡°Ý¹®ÀÇ
Mirus
MIR 6775
VirusGEN¢ç SELECT AAV Transfection Kit
1 Kit (50 L cell culture)
°¡°Ý¹®ÀÇ
Mirus
MIR 6815-GMP
VirusGEN¢ç GMP AAV Transfection Kit
1 Kit (50 L cell culture)
°¡°Ý¹®ÀÇ
Product index
Overview
[FAQ] Mirus transfecti..
Cell lineº° ¼±Åð¡À̵å
PACKAGE DELIVERY: The ..
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀÔ..
TransIT-X2¢ç Transfect..
TransIT¢ç-LT1 Transfec..
TransIT¢ç-2020 Transfe..
Xfect¢â Transfection R..
CalPhos¢â Mammalian Tr..
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀÔ½Ã..
TransIT-VirusGEN¢ç Tra..
TransIT¢ç-Lenti Transf..
TransIT-PRO¢ç Transfec..
VirusGEN¢ç AAV Transfe..
VirusGEN¢ç LV Transfec..
¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯Àü..
TransIT¢ç-293 Transfec..
TransIT¢ç-BrCa Transfe..
TransIT¢ç-CHO Transfec..
TransIT-HeLaMONSTER¢ç ..
TransIT¢ç-Jurkat Trans..
TransIT¢ç-Keratinocyte..
TransIT¢ç-Insect Trans..
Xfect mESC Transfectio..
Electroporation °ü·Ã
Ingenio¢ç solution & k..
Ingenio¢ç cuvette, ¼Ò..
µµÀÔ¹°Áú¿¡ µû¸¥ µµÀԽþà
TransIT¢ç-mRNA Transfe..
TransIT¢ç-Oligo Transf..
Xfect¢â RNA Transfecti..
Xfect¢â Protein Transf..
in-vivo delivery ½Ã½ºÅÛ
TransIT Hydrodynamic D..
µ¶¼º Á¦°Å - Transfection ..
MiraCLEAN¢ç Endotoxin ..
siRNA Transfection ½Ã¾à
Label IT Delivery Cont..
Label IT siRNA Tracker..
TransIT-QR Hydrodynami..
TransIT-siQUEST Transf..
TransIT-TKO Transfecti..
TransIT-X2 Dynamic Del..
siRNA ¹ßÇö ¡¤ ºÐ¼®
pSINsi vector ½Ã¸®Áî
Synthetic siRNA Quanti..
small RNA Cloning
pBApo-CMV vector ½Ã¸®..
pBApo-EF1¥á Vector ½Ã..
small RNA Cloning Kit
small RNA Gel Extracti..
RNAi °ü·Ã Kit
14-30 ssRNA Ladder Mar..
siRNA Ladder Marker
*VirusGEN
®
SELECT ¹× VirusGEN
®
GMP kitÀÇ °ßÀû ¹× ±¸¸Å´Â ¹Ýµå½Ã ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø (T. 02-2081-2510)À¸·Î ¹®ÀÇ ¹Ù¶ø´Ï´Ù.
³ôÀº °¨¿°È°¼ºÀ» °¡Áö´Â °íǰÁúÀÇ Adeno associated virus (AAV) »ý»ê¿¡ ÃÖÀû
HEK293 ¼¼Æ÷ ¹è¾ç ½Ã½ºÅÛ¿¡ ÃÖÀû (adherent ¹× suspension ¹è¾ç ¸ðµÎ Àû¿ë)
¿¬±¸¿ë ½Ã¾àºÎÅÍ ÀüÀÓ»ó ´Ü°è, ÀÓ»ó¿ë GMP grade±îÁö ¶óÀξ÷
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IT-VirusGEN
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Transfection Reagent´Â expression vector¿Í packaging plasmid¸¦ HEK293 ŸÀÔÀÇ ÆÐŰ¡ ¼¼Æ÷·Î È¿À²ÀûÀ¸·Î µµÀÔÇÏ¿©, AAV ¶Ç´Â LentivirusÀÇ »ý»êÀ²À» ³ô¿©ÁÖ´Â transfection ½Ã¾àÀÌ´Ù.
±× Áß¿¡¼µµ º» VirusGEN
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AAV Transfection Kit Á¦Ç°Àº
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Transfection Reagent¸¦ ±â¹ÝÀ¸·Î plasmid µµÀÔ È¿À²À» ³ôÀ̱â À§ÇÑ VirusGEN
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AAV Complex Formation Solution and Enhancer°¡ Ãß°¡µÇ¾î AAVÀÇ È¿À²ÀûÀÎ »ý»êÀ» À§ÇØ ÃÖÀûÈµÈ Á¦Ç°ÀÌ´Ù.
VirusGEN
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SELECT AAV Transfection Kit´Â ÀüÀÓ»ó ¿¬±¸ ¹× °øÁ¤ °³¹ß¿¡¼ ÇÊ¿äÇÑ ´ë±Ô¸ð ¹ÙÀÌ·¯½º »ý»êÀ» À§ÇÑ ÃÖÀûÀÇ ½Ã¾àÀ¸·Î °£ÆíÇÏ°í ºñ¿ë È¿À²ÀûÀÎ ½ÇÇè ÇÁ·Î¼¼½º¸¦ Á¦°øÇÑ´Ù. ¶ÇÇÑ ÀüÀÓ»ó¿ë ¹ÙÀÌ·¯½º º¤ÅÍ Á¦ÀÛÀ» À§ÇÑ transfection ½Ã¾àÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖµµ·Ï ǰÁú º¸Áõ ½ÃÇèÀ» ¿Ï·áÇÏ¿´´Ù.
([
Example Certificate of Origin (PDF)
|
Example Certificate of Analysis (PDF)
|
Example BSE TSE Statement (PDF)
])
µû¶ó¼, ÀÓ»ó ½ÃÇè ¶Ç´Â »ó¾÷ÀûÀÎ ¹ÙÀÌ·¯½º »ý»êÀ» À§ÇØ VirusGEN
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GMP AAV Transfection Kit¸¦ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
±×¸²1. Ÿ»ç´ëºñ ³ôÀº AAV functional titer
Suspension ÇüÅ·Π¹è¾çÇÑ Freestyle 293-F¼¼Æ÷ (FreeStyle F17 Medium¿¡¼ ¹è¾ç)(A)¿Í Adherent ÇüÅ·Π¹è¾çÇÑ 293T/17¼¼Æ÷(B)¿¡
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Transfection Reagent (reagent : DNA (vol : wt) = 2 : 1), Ÿ»ç B4, Ÿ»ç B5, Ÿ»ç C3 (25kDa PEI)¸¦ »ç¿ëÇÏ¿© pAAV-hrGFP, pAAV-RC, pAAV-Helper (1 : 1 : 1 ratio, 1.5 §¶/§¢, Agilent Technologies)¸¦ transfection ÇÏ¿© ¹ÙÀÌ·¯½º¸¦ »ý»êÇÏ¿´´Ù. »ý»êµÈ ¹ÙÀÌ·¯½º´Â HT1080¼¼Æ÷¿¡ °¨¿°, 48½Ã°£ µÚ GFP¹ßÇöÀ» À¯¼¼Æ÷ÃøÁ¤±â·Î ÃøÁ¤ÇÏ¿´´Ù.
*Functional titer´Â GFP ¹ßÇö¼¼Æ÷°¡ 20% ¹Ì¸¸ÀÎ virus Èñ¼®¾×À¸·ÎºÎÅÍ ÃøÁ¤ÇÏ¿´°í, error bar´Â 3¹Ýº¹ ½ÇÇ豺ÀÇ Ç¥ÁØÆíÂ÷ÀÌ´Ù.
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IT-VirusGEN
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Reagent, VirusGEN
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AAV Transfection Kit - Ÿ»ç Á¦Ç°°úÀÇ »ý»êÈ¿À² ºñ±³
Trans
IT-VirusGEN
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Reagent¿Í VirusGEN
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AAV Transfection Kit, Ÿ»ç A1, Ÿ»ç A3À» ÀÌ¿ëÇÏ¿© AAV2ÀÇ »ý»êÈ¿À²À» ºñ±³ÇÏ¿´´Ù. Expi293 Expression Media (Thermo Fisher Scientific)¿¡¼ ¹è¾çÇÑ Expi293F cells (Thermo Fisher Scientific)¿¡ pAAV-hrGFP, pAAV-RC, pAAV-Helper plasmids (1:1:1 DNA ratio, 2 §¶/§¢ - 2 §· per flask, Agilent Technologies)¸¦ µµÀÔÇϰí 72½Ã°£ ÈÄ lysis ½ÃÄÑ AAV¸¦ ȸ¼öÇÏ¿´´Ù.
A. ȸ¼öÇÑ ¹ÙÀÌ·¯½º¸¦ HT10180¼¼Æ÷¿¡ °¨¿°½Ã۰í 48½Ã°£ ÈÄ¿¡ GFPÀÇ ¹ßÇöÀ» È®ÀÎÇÏ¿© ¿ª°¡¸¦ ÃøÁ¤ÇÏ¿´´Ù. ¶Ç µµÀÔµÈ AAVÀÇ genome copy number´Â CMV promoter ¿µ¿ªÀ» ddPCR·Î È®ÀÎÇÏ¿´´Ù.
B. Á¦ÀÛµÈ ¹ÙÀÌ·¯½º capsid¼ö´Â AAV2 Titration ELISA Kit (Progen)À» ÀÌ¿ëÇÏ¿© ÃøÁ¤Çß´Ù. ¶Ç GC/capsidÀÇ ºñÀ²À» °è»êÇÏ¿´´Ù
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Reagent, VirusGEN
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AAV Transfection Kit¸¦ ÀÌ¿ëÇßÀ» ¶§ ´õ ¸¹Àº ¿ª°¡ÀÇ AAV¸¦ »ý»êÇÒ ¼ö ÀÖ¾ú°í, ƯÈ÷ VirusGEN
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AAV Transfection Kit¸¦ ÀÌ¿ëÇÏ¿´À» ¶§ °¡Àå ¸¹Àº ¾çÀÇ AAV¸¦ »ý»êÇÒ ¼ö ÀÖ¾ú´Ù.
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IT-VirusGEN
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Transfection Reagent: -10~-30¡É º¸Á¸
VirusGEN
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AAV Complex Formation Solution and Enhancer : 2~10¡É
(SELECT, GMP ¸ðµÎ µ¿ÀÏ)
°ü·ÃÁ¦Ç°
- ´Ù¾çÇÑ SerotypeÀÇ vector system:
AAVpro
®
Helper Free System (AAV1, 2, 5, 6)
- CRISPR/Cas9 Vector System:
AAVpro
®
CRISPR/Cas9 Helper Free System (AAV2)
- AAV extraction:
AAVpro
®
Extraction Solution
- Cell¿¡¼ Á¤Á¦:
AAVpro
®
Purification Kit (All Serotypes
)
- ¹è¾ç¹èÁö¿Í cell¿¡¼ ¸ðµÎ Á¤Á¦:
AAVpro
®
Cell & Sup. Purification Kit Maxi (All Serotypes)
- AAV titration:
AAVpro
®
Titration Kit (for Real Time PCR)
- Packaging (293T Cell Line):
AAVpro
®
293T Cell Line
Keyword :
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀԽþà
°í¿ª°¡ÀÇ AAV, Lentivirus »ý»êÀ» À§ÇÑ (GMP Ãâ..
Trans
IT-VirusGEN¢ç Transfection Reagent
Recombinant Lentivirus »ý»ê¿¡ ÃÖÀû
Trans
IT¢ç-Lenti Transfection Reagent
Trans
IT-PRO¢ç Transfection Reagent & Kit
AAV »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç AAV Transfection Kit
Lentivirus »ý»ê¿ë enhancer Ãß°¡ (GMP Ãâ½Ã)
VirusGEN¢ç LV Transfection Kit