´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.

Poly(A) Polymerase

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2180A
Poly(A) Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 U
109,000¿ø  2180A_DS.v2107Da.pdf
Takara
2180B
Poly(A) Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 U (2180A x 5)
485,000¿ø 

Á¦Ç°¼³¸í
º» È¿¼Ò´Â ´Ù¾çÇÑ polynucleotideÀÇ 3¡¯-¸»´Ü¿¡ adenyl Àܱ⸦ ÁßÇÕÇÏ´Â È¿¼ÒÀÌ´Ù. ´ÜÀÏ °¡´Ú RNA¸¦ primer·Î »ç¿ëÇÒ ¼ö ÀÖÁö¸¸ ÀÌÁß °¡´Ú RNA, ÇÕ¼º polynucleotide, ªÀº oligonucleotide µîÀº primer·Î »ç¿ëÇϱ⠾î·Æ°í DNA´Â primer·Î ±â´ÉÇÏÁö ¾Ê´Â´Ù. ÀÌ È¿¼Ò´Â AMP Àܱ⸦ ÁßÇÕÇÏÁö¸¸ ATP¸¸À» ±âÁú·Î ÀÌ¿ëÇϱ⿡, ADP, dATP´Â ±âÁúÀÌ µÉ ¼ö ¾ø½À´Ï´Ù. UTP, CTPÀÇ ±âÁú ÀÌ¿ëÀ²Àº ATPÀÇ 5% ÀÌÇÏÀ̸ç GTP´Â ÁßÇÕÇÒ ¼ö ¾ø½À´Ï´Ù.
º¸Á¸
-20¡É
³óµµ
2 U/§¡
Çü»ó

25 mM

Tris-HCl (pH7.9)

500 mM

NaCl

1 mM

EDTA

0.1 mM

DTT

50%

Glycerol

ÁÖÀÇ
º» Á¦Ç°¿¡´Â ¹ÝÀÀ¿ë buffer°¡ ÷ºÎµÇ¾î ÀÖÁö ¾Ê½À´Ï´Ù.
È°¼ºÀÇ Á¤ÀÇ
37¡É, pH7.9¿¡¼­ ATP¸¦ ±âÁú·Î ÇÏ¿© 10 ºÐ°£ 1 nmolÀÇ AMP¸¦ primer¿¡ ÁßÇÕ ÇÏ´Â È¿¼ÒÈ°¼ºÀ» 1UÀ¸·Î ÇÑ´Ù.
È°¼º ÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º

50 mM

Tris-HCl (pH7.9)

10 mM

MgCl2

2.5 mM

MnCl2

250 mM

NaCl

1 mM

DTT

0.05%

BSA

400 §¶/§¢

tRNA

0.1 mM

[3H]ATP

¿ëµµ
- RNA¿¡ poly(A) tail ºÎ°¡
- RNAÀÇ 3¡¯-¸»´Ü Ç¥½Ä
±â¿ø
Escherichia coli B
ÀϹÝÀû ¼ºÁú
- ºÐÀÚ·®: ¾à 58,000
- ÃÖÀû pH: pH8.0
- Subunit
   Mg2+, Mn2+¸¦ ÇÊ¿ä·Î Çϸç, °¢°¢ 10 mM Mg2+, 2.5 mM Mn2+ÀÇ ³óµµ·Î °øÁ¸ÇÒ ¶§ ÃÖ°íÀÇ È°¼ºÀ» ³ªÅ¸³½´Ù.
- ¹ÝÀÀ ½Ã°£¿¡ µû¸¥ ATPÀÇ ºÎ°¡ °³¼ö
   84 §¶ÀÇ RNA¿¡ ATP 1 mMÀ» ±âÁú·Î 15 UÀÇ Poly(A) Polymerase¸¦ ¹ÝÀÀÇÑ °æ¿ì

¹ÝÀÀ ½Ã°£(ºÐ)

0

5

10

30

60

Poly(A) bases

0

10

20

40

75


Keyword :

in vitro transcription
>
°íÇ°Áú °í¼öÀ² in vitro transcriptionÀ» À§ÇÑ ..
Takara IVTpro¢â mRNA Synthesis System
>
dsRNA »ý¼ºÀÌ Àû¾î ÀÓ»óºÐ¾ßÀÇ ¿¬±¸°³¹ß¿¡ ÀûÇÕ..
PrimeCap¢â T7 RNA Polymerase (low dsRNA)
>
Poly(A) ÀÌÈÄ Ãß°¡¼­¿­ÀÌ ¾ø´Â scarless mRNA ..
Template Vector (BspQ I) for T7 mRNA Synthesis
>
RNAÀÇ 5¡¯-¸»´Ü¿¡ Cap (Cap 0)À» ºÎ°¡ÇÏ´Â È¿¼Ò
Vaccinia Capping Enzyme
>
RNAÀÇ 5¡¯ ¸»´ÜÀ» Cap 0 ¡æ Cap 1·Î º¯È¯ÇÏ´Â ..
mRNA Cap 2-O-Methyltransferase
>
´Ù¾çÇÑ ¿Âµµ¿¡¼­ ¹ÝÀÀÇÒ ¼ö ÀÖ´Â Capping enzym..
Faustovirus Capping Enzyme (S17)
>
Poly(A) ¹× ¹Ýº¹ ¼­¿­À» Æ÷ÇÔÇÏ´Â À¯ÀüÀÚÀÇ Å¬..
Takara Stable Competent Cells
>
RNA ÇÕ¼º ¼öÀ²ÀÌ Çâ»óµÈ
T7 RNA Polymerase ver.2.0
>
Àú³óµµ DTT¿¡¼­µµ ¾ÈÁ¤ÀûÀÎ RNase inhibitor
Recombinant RNase Inhibitor ver.2.0
>
DNA, RNA ÇÕ¼º ¹ÝÀÀÀ» ÃËÁø
Pyrophosphatase (inorganic)
>
IVT Àû¿ë¿¡ ÃÖÀû, Type IIS Á¦ÇÑÈ¿¼Ò
BspQ I
>
´Ù¾çÇÑ Å©±âÀÇ ssRNA »çÀÌÁî È®ÀÎ
0.5-10 kb ssRNA Ladder Marker
>
BspQ IÀ» »ç¿ëÇÑ °í¼öÀ² IVT ½Ã½ºÅÛ
(BspQ I) IVTpro¢â ½Ã½ºÅÛ
>
Low dsRNA Polymerase¸¦ »ç¿ëÇÑ °í¼öÀ² IVT ½Ã..
(PrimeCap¢â T7) IVTpro¢â ½Ã½ºÅÛ