Á¦Á¶»ç |
Á¦Ç°ÄÚµå |
Á¦Ç°¸í |
¿ë·® |
°¡°Ý (ºÎ°¡¼¼º°µµ) |
ºñ°í |
»ç¿ëÀڸŴº¾ó |
Clontech
|
634485
|
SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian
|
24 ȸ
|
4,577,000¿ø
|
|
|
Clontech
|
634486
|
SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian
|
48 ȸ
|
7,282,000¿ø
|
|
Clontech
|
634487
|
SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian
|
96 ȸ
|
9,596,000¿ø
|
|
Clontech
|
634488
|
SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian
|
192 ȸ
|
17,154,000¿ø
|
|
Product index
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PGT-A
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SMARTer RNA-Seq °³¿ä
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mRNA-Seq (Single cell)
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Stranded RNA-seq
-
Small RNA-Seq
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Immune-Seq
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±âŸ RNA-Seq
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DNA-seq °³¿ä
-
ChIP-Seq / Meth-Seq
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Low Input DNA-Seq
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High input DNA-seq
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Single cell DNA-Seq
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Whole Genome Amplification..
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Index Kit
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NGS Library Á¤·®
-
NGS Sample preparation
-
NGS ºÐ¼®¿ë PCR È¿¼Ò
-
NGS °ü·Ã Àåºñ
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[Legacy] NGS Products
* º» Á¦Ç°Àº SMARTer¢ç Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Code 634411)ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°ÀÔ´Ï´Ù.
* º» Á¦Ç°Àº 24, 48, 96, 192ȸ Á¦Ç°À¸·Î Á¦°øµÇ¸ç, SMARTer RNA Unique Dual Index Kit - 24U (Code 634451, 96ȸ)°¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù. SMARTer¢ç RNA Unique Dual Index Kit - 96U Set A/B (Code 634452/634457, °¢ 96ȸ)À» º°µµ·Î ±¸¸ÅÇϸé ÃÖ´ë 192°³ÀÇ ´Ù¸¥ index¸¦ Á¶ÇÕÇÑ library¸¦ ±¸ÃàÇÒ ¼ö ÀÖ½À´Ï´Ù.
- pg ¼öÁØÀÇ ±Ø¼Ò·® »ùÇà Àû¿ë - Human, mouse, rat À¯·¡ÀÇ total RNA (250 pg - 10 ng) ȤÀº intact cell (10-1,000°³)
- UMI Àû¿ëÀ¸·Î ºÐ¼® Á¤È®µµ Áõ°¡ - »ùÇà ³» true variant¿Í rare mutationÀÇ Á¤È®ÇÑ ºÐ¼® ¹× PCR ÁõÆø°úÁ¤À¸·Î ¹ß»ýÇÒ ¼ö ÀÖ´Â bias³ª sequencing error ¹æÁö
- FFPC³ª LCM, cell-free RNA¸¦ Æ÷ÇÔÇÏ´Â ³·Àº quality RNA¿¡¼ ÀçÇö¼º ³ôÀº °á°ú È®ÀÎ
- ºü¸£°í °£ÆíÇÑ ÇÁ·ÎÅäÄÝ - Buffer Á¶¼ºÀÇ ¾÷±×·¹À̵å·Î library Á¤Á¦ ½Ã°£ °¨Ãà, Àü °úÁ¤ÀÌ 7.5½Ã°£ ³» ¿Ï·á
- Illumina¢ç Ç÷§Æû ȣȯ - Illumina¢ç ºÐ¼®¿ë index°¡ Àû¿ëµÇ¾î ÀÖÀ¸¸ç, ÃÖ´ë 192°³ÀÇ Á¶ÇÕ °¡´É
Á¦Ç° ¼³¸í
SMARTer¢ç Stranded Total RNA - Seq Kit v3 - Pico Input Mammalian´Â human, rat, mouse À¯·¡ÀÇ total RNA (250 pg ~ 10 ng) ȤÀº intact cell (10-1,000°³)·ÎºÎÅÍ Strand-specific Illumina¢ç NGS library¸¦ È¿À²ÀûÀ¸·Î Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº ±Ø¹Ì·®ÀÇ total RNA¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖÀ» »Ó¸¸ ¾Æ´Ï¶ó FFPE, LCM »ùÇðú °°ÀÌ RNA integrity°¡ ÇöÀúÈ÷ ³·Àº »ùÇÿ¡¼µµ NGS Library Á¦ÀÛÀÌ °¡´ÉÇÏ´Ù. º» Á¦Ç°Àº library Á¦ÀÛ °úÁ¤ ³»¿¡ unique molecular identifiers (UMIs)¸¦ Ãß°¡ µµÀÔÇÔÀ¸·Î½á ±âÁ¸ÀÇ Version 2 Á¦Ç°¿¡ ºñÇØ Illumina¢ç Sequencing È¿À²°ú Á¤È®µµ¸¦ °³¼±ÇÏ¿´´Ù.
SMARTer¢ç Stranded Total RNA - Seq Kit v3 - Pico Input Mammalian¸¦ ÀÌ¿ëÇϸé, ¿ªÀü»ç¹ÝÀÀ°ú PCR ÁõÆø ´Ü°è¿¡¼ index¿Í adapter¸¦ ºÎ°¡Çϸç, strand Á¤º¸¸¦ º¸À¯ÇÏ´Â library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. Library prep ¹× Á¤Á¦ °úÁ¤À» Æ÷ÇÔÇÑ Àü ½ÇÇè °úÁ¤Àº 7.5½Ã°£ ³» ¿Ï·áµÈ´Ù.
º» Á¦Ç°Àº Mammalian rRNA¿Í mitochondrial RNA ƯÀÌÀûÀÎ Probe·Î rRNA derived cDNA¸¦ Á¦°ÅÇÏ¿© º¸´Ù ³ôÀº mapping statistics¸¦ ¾òÀ» ¼ö ÀÖ´Â ±â¼úÀÌ Àû¿ëµÇ¾î ÀÖ´Ù. Ribosomal RNA (rRNA)´Â total RNAÀÇ ¾à 90%¸¦ Â÷ÁöÇÏ´Â RNA·Î½á NGS library Á¦ÀÛ Àü, cDNA¿¡¼ rRNA¸¦ Á¦°Å (depletion)ÇÔÀ¸·Î½á Á¦ÀÛºñ¿ëÀ» Àý°¨ÇÒ ¼ö ÀÖ°í, mapping statistics ¶ÇÇÑ Çâ»ó½Ãų ¼ö ÀÖ´Ù. ÇÏÁö¸¸ ¸Å¿ì ±Ø¼Ò·®ÀÇ Total RNA¸¦ ÀÌ¿ëÇÒ °æ¿ì, ÀϹÝÀûÀÎ rRNA depletion ¹æ¹ýÀ» Àû¿ëÇϸé NGS library¸¦ Á¦Á¶ÇÏ´Â µ¥ ÇÊ¿äÇÑ RNA ¾ç¿¡ ¹ÌÄ¡Áö ¸ø ÇÏ°Ô µÈ´Ù.

±×¸² 1. Schematic of technology in the SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian

±×¸² 2. SMARTer¢ç Stranded Total RNA-Seq Kit v2 - Pico Input MammalianÀ» ÀÌ¿ëÇÏ¿© Á¦ÀÛÇÑ NGS LibraryÀÇ ±¸Á¶
The adapters added using the SMARTer¢ç RNA Unique Dual Index Kit - 24U (Code 634451) contain sequences allowing clustering on any Illumina flow cell (P7 shown in light blue, P5 shown in red, Index 1 [i7] sequence shown in orange, and Index 2 [i5] sequence shown in yellow), as well as the regions recognized by sequencing primers Read Primer 2 (Read 2, purple) and Read Primer 1 (Read 1, green). Read 1 generates sequences antisense to the original RNA, while Read 2 yields sequences sense to the original RNA (orientation of original RNA denoted by 5' and 3' in dark blue). The first 8 nt of the second sequencing read (Read 2) are UMIs (dark purple) followed by 3 nucleotides derived from the Pico v3 SMART UMI Adapter (shown as XXX).
Applications
- Robust NGS library construction that retains strand information
- RNA-seq on all Illumina platforms
- Coding and noncoding information from total mammalian RNA of any quality, including RNA obtained from FFPE samples
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
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Keyword : BioMarker,FFPE,Cell-free RNA,Degraded RNA,Non-coding RNA (ncRNA),Long non-coding RNA (lncRNA),Viral infection,Viral Genetics,COVID-19,Liquid biopsy,Cancer,Omics,Transcriptomics,Extracellular Vesicle,EV,Exosome,Whole transcriptome,LCM-RNAseq,digital spatial profiling,spatial transcriptomics,stranded,stranded-seq,smart,smarter,smart-seq,random primer