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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > [Legacy] NGS Products > (Legacy) PicoPLEX¢ç WGA Kit
Single cell, 10pg DNA - Whole Genome Amplification (WGA)

(Legacy) PicoPLEX¢ç WGA Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
R30050
PicoPLEX¢ç WGA Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
50 ȸ
°¡°Ý¹®ÀÇ   RB3050 Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x99914

  • Single cell, ±Ø¼Ò·® gDNA·ÎºÎÅÍ ¶óÀ̺귯¸® ±¸ÃàÀ» À§ÇÑ Whole Genome Amplification (WGA) Kit
  • Single cell ¶Ç´Â 6pgÀÇ gDNA·ÎºÎÅÍ ¾ÈÁ¤ÀûÀÎ ÁõÆø °á°ú
  • ³ôÀº ÀçÇö¼ºÀÇ allele representation
  • WGA ±â¼ú·Î ÁõÆøÇÑ DNA´Â NGS, Microarray, PCR µî ´Ù¾çÇÑ ¹æ¹ýÀ¸·Î ºÐ¼®
Á¦Ç°¼³¸í
PicoPLEX¢ç WGA KitÀº ´ÜÀÏ ¼¼Æ÷(single cell)·Î ºÎÅÍ ³ôÀº ÀçÇö¼ºÀ» °¡Áø ¶óÀ̺귯¸®¸¦ ¸¸µé±â À§ÇØ DNA¸¦ ÁõÆøÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°ÀÇ ±âº»ÀÌ µÇ´Â °íÀ¯ÀÇ ±â¼ú(ƯÇã ±â¼ú), PicoPLEX¢ç ±â¼úÀº single copy genomic DNAÀÇ ÁõÆø¿¡ ÃÖÀûÈ­µÇ¾î ÀÖ´Ù. ÇϳªÀÇ tube¿¡¼­ ¹ÝÀÀÇÏ´Â single tube reactionÀ¸·Î »ç¿ëÇÏ±â Æí¸®Çϰí, ¿¡·¯¸¦ ÃÖ¼ÒÈ­ ÇÒ ¼ö ÀÖÀ¸¸ç, ¹é±×¶ó¿îµå¸¦ ÃÖ¼ÒÈ­ÇÏ¿© Çâ»óµÈ °á°ú¸¦ µµÃâÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº single cell (1 - 10 cells)»Ó ¾Æ´Ï¶ó, ÃßÃâÇÑ ±Ø¼Ò·®(6 - 50 pg)ÀÇ gDNA·ÎºÎÅÍ Whole Genome Amplification (WGA)¸¦ ÁøÇàÇÒ ¼ö ÀÖÀ¸¸ç, ÁõÆøÇÑ DNA´Â PCRÀº ¹°·Ð array CGH analysis¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
PicoPLEX¢ç WGA KitÀº in vitro fertilization(IVF, ü¿Ü¼öÁ¤)½Ã pre-implantation genetic screening and diagnostics (PGS/PGD)¿Í °°Àº °Ë»ç ½Ã À¯¿ëÇÏ°Ô »ç¿ëµÇ°í ÀÖ´Ù.


[±×¸² 1] PicoPLEX¢ç WGA±â¼úÀÇ ¿ø¸®
Cells are lysed, quasi-random primers pre-amplify the DNA selectively, and a final PCR amplification adds the Illumina barcodes. (PicoPLEX¢ç ±â¼ú °³¿ä º¸±â)


[±×¸² 2] PicoPLEX¢ç WGA Kit - Single tube workflow


[±×¸²3] Amplification of Single Cells is Reproducible
Flow-sorted cancer cells were amplified with the PicoPLEX¢ç WGA Kit. Each of the cells (blue lines) amplified at the same rate, and resulted in a similar, predictable yield. The sample containing 0 cells (green lines; no template control) shows very low background.


[±×¸²4] Reproducible Copy Number Variation(CNV) data
Single-blastomere biopsies were amplified using PicoPLEX¢ç WGA Kit, labeled and hybridized to BlueGnome¡¯s 24Sure arrays at Genesis Genetics Institute. Note clear indication of CNVs. In 2011, ESHRE clinical trials confirmed accuracy of karyotyping using PicoPLEX¢ç technology.
º¸°ü
Store at -20 ¡ÆC.
Guarantee for 12 months at -20¡ÆC in a constant temperature freezer.
³»¿ë : 50 ȸºÐ

Component Name

Tube Cap Color

Cell Extraction Buffer

Green

Extraction Enzyme Dilution Buffer

Violet

Cell Extraction Enzyme

Yellow

Pre-Amp Buffer

Red

Pre-Amp Enzyme

White

Amplification Buffer

Orange

Amplification Enzyme

Blue

Nuclease-Free Water

Clear


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