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Home > 전제품보기 > NGS 관련 > Total RNA-Seq with strand info > SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian

High Input Strand- Specific Total RNA-Seq

SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian

제조사	제품코드	제품명	용량	가격 (부가세별도)
Clontech	634873	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	12 회	2,199,000원
Clontech	634874	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	24 회	3,760,000원
Clontech	634875	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	48 회	5,958,000원
Clontech	634876	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	96 회	8,624,000원
Clontech	634877	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	192 회	16,834,000원
Clontech	634878	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	480 회	40,953,000원

SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian은 100ng - 1ug의 mammalian Total RNA로부터 Illumina용 sequencing library를 빠른 시간 내에 제작할 수 있다. 본 제품은 Total RNA의 90%를 이루는 ribosomal RNA (rRNA)를 제거를 위한 RiboGone (Code 634846)과 SMARTer 기술이 적용되어있어 Total RNA로부터 약 5시간만에 rRNA depletion과 strand-specific library를 제작할 수 있다.
SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian은 일반적인 양 (High-input, >100 ng)을 가진 RNA로부터 5시간만에 Illumina specific, indexed RNA Seq library를 제작하는 제품으로, 12개의 Reverse primer와 8개의 Forward primer를 포함하고 있어 총 96개의 indexed library를 제작 가능하다.

미량의 샘플(Low input, 100 pg- 100 ng)의 total RNA 샘플을 이용하는 경우, SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian (Code 634861)을 권장한다.

특징

Strand information - Identify each transcript’s strand of origin with >99% accuracy.
Fast, streamlined protocol - Go from start to finish in ~5 hours.
Large input size - Use 100 ng-1 μg total RNA from human, mouse, or rat.
Versatility - Get reliable data across replicates and RNA quality. Seehigh - quality, reproducible data from total RNA with a RIN (RNA IntegrityNumber) from 3-10.
Integrated with Illuminasequencing - Generate Illumina - specificlibraries with up to 96 indexes, and retain strand information.

그림 1. Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification.

표 1. High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

표 2. Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

적용

Illumina-specific NGSlibrary generation that retains strand information
RNA-Seq on Illuminaplatforms for mammalian samples
Coding and non-codingtranscript information

구성품 (자세한 구성품은 CoA 또는 매뉴얼 참조)

- SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian Components
- Indexing Primer Set HT for Illumina - 12
- SeqAmp DNA Polymerase

Keyword : SMART,NGS,RNA-Seq,Illumina

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