·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
|
Á¦Ç°»ó´ã¹®ÀÇ
°í°´Áö¿ø
¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
02-2081-2510
Email ¦¢
support@takara.co.kr
´ëÀüÁö»ç
¾÷¹«¾È³»¦¢´ëÀü/ÃæÃ»Áö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
042-828-6525
Email ¦¢
tkbd@takara.co.kr
¾÷¹«½Ã°£¾È³»
[ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Products
°øÁö»çÇ×
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
樨毢
ȸ»ç¼Ò°³
That's Good Science!
Á¦Ç°¼±Åð¡À̵å
ÀüÁ¦Ç°º¸±â
Custom Service
COVID-19
À̺¥Æ®/Ä·ÆäÀÎ
´ÙÄ«¶ó¼Ò½Ä
´ÙÄ«¶ó°ø½Ã
½ÅÁ¦Ç°Á¤º¸
´ÙÄ«¶ó ¶óÀ̼±½º
±â¼úÁö¿ø¹®ÀÇ
Á¤º¸ÀÚ·á½Åû
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
LS&B
ºê·Î¼Å
eNews
CoA & MSDS
¿Â¶óÀÎÅø
樨毢
¿Â¶óÀμ¼¹Ì³ª
ȸ»ç¼Ò°³
µµÀԺ귣µå ¾È³»
´ë¸®Á¡¾È³»
ã¾Æ¿À½Ã´Â±æ
Welcome
About Thats Good Science
Watch the Videos - S3
Watch the Videos - S2
Watch the Videos - S1
Home
>
ÀüÁ¦Ç°º¸±â
>
NGS °ü·Ã
>
Stranded RNA-seq
> HI Mammalian
High Input Strand- Specific Total RNA-Seq
HI Mammalian
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Clontech
634873
SMARTer¢ç Stranded Total RNA Sample Prep Kit - HI Mammalian
12 ȸ
2,205,000¿ø
Clontech
634874
SMARTer¢ç Stranded Total RNA Sample Prep Kit - HI Mammalian
24 ȸ
3,723,000¿ø
Clontech
634875
SMARTer¢ç Stranded Total RNA Sample Prep Kit - HI Mammalian
48 ȸ
6,561,000¿ø
Clontech
634876
SMARTer¢ç Stranded Total RNA Sample Prep Kit - HI Mammalian
96 ȸ
8,930,000¿ø
Clontech
634877
SMARTer¢ç Stranded Total RNA Sample Prep Kit - HI Mammalian
192 ȸ
17,194,000¿ø
Product index
PGT-A
Embgenix¢â PGT-A Kit (..
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
[FAQ] RNA-seq
Cancer ¿¬±¸ °¡À̵å
Cell-free DNA/RNA °¡ÀÌ..
RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸..
SMARTer RNA-seqÀÇ HOT ..
mRNA-Seq (Single cell)
[Cancer Research] Canc..
Single cell-seq °¡À̵å
Single cell SMART-seq..
Single cell RNA-seq¿¡ ..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
´Ù·®ÀÇ ±Ø¼Ò·® »ùÇÃÀ» ..
±Ø¼Ò·® mRNAÀÇ 3¡¯ ¸»´Ü..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
Stranded RNA-seq
Single cell Stranded K..
[NEW] Pico Input Mamma..
Pico Input Mammalian (..
Low Input Mammalian
HI Mammalian
Stranded RNA-Seq Kit
Total RNA-Seq Kit (FFP..
Small RNA-Seq
[Cancer Research] Canc..
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[Cancer Research] Immu..
[Version 2] Human TCR ..
Human TCR a/b Profilin..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
SMART-Seq Human BCR (w..
SMART-Seq Human TCR (w..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
Cell-free DNA/RNA °¡ÀÌ..
Single cell-seq °¡À̵å
[FAQ] DNA-Seq
[Àû¿ë] Overview_ThruPL..
[Cancer Research] Canc..
DNA-seqÀÇ ´Ù¾çÇÑ ÃֽŠ..
ChIP-Seq / Meth-Seq
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
[³í¹®] ThruPLEX¢ç for ..
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
[Cancer Research] Canc..
Single cell-seq °¡À̵å
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
[Cancer Research] HLA ..
[³í¹®] PicoPLEX¢ç ±â¼ú..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
[FAQ] Library index
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
Unique Dual Index Kits
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Citation
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) SMARTer Ultra..
(Legacy) SMARTer¢ç Str..
(Legacy) PicoPLEX¢ç DN..
(Legacy) ThruPLEX¢ç Pl..
(Legacy) ThruPLEX¢ç DN..
(Legacy) PicoPLEX¢ç WG..
SMARTer Stranded Total RNA Sample Prep Kit - HI MammalianÀº 100ng - 1ugÀÇ mammalian Total RNA·ÎºÎÅÍ Illumina¿ë sequencing library¸¦ ºü¸¥ ½Ã°£ ³»¿¡ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº Total RNAÀÇ 90%¸¦ ÀÌ·ç´Â ribosomal RNA (rRNA)¸¦ Á¦°Å¸¦ À§ÇÑ
RiboGone (Code 634846)
°ú SMARTer ±â¼úÀÌ Àû¿ëµÇ¾îÀÖ¾î Total RNA·ÎºÎÅÍ ¾à 5½Ã°£¸¸¿¡ rRNA depletion°ú strand-specific library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù.
SMARTer Stranded Total RNA Sample Prep Kit - HI MammalianÀº ÀϹÝÀûÀÎ ¾ç (High-input, >100 ng)À» °¡Áø RNA·ÎºÎÅÍ 5½Ã°£¸¸¿¡ Illumina specific, indexed RNA Seq library¸¦ Á¦ÀÛÇÏ´Â Á¦Ç°À¸·Î, 12°³ÀÇ Reverse primer¿Í 8°³ÀÇ Forward primer¸¦ Æ÷ÇÔÇϰí ÀÖ¾î ÃÑ 96°³ÀÇ indexed library¸¦ Á¦ÀÛ °¡´ÉÇÏ´Ù.
¹Ì·®ÀÇ »ùÇÃ(Low input, 100 pg- 100 ng)ÀÇ total RNA »ùÇÃÀ» ÀÌ¿ëÇÏ´Â °æ¿ì,
SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian (Code 634861)
À» ±ÇÀåÇÑ´Ù.
Ư¡
Strand information
- Identify each transcript¡¯s strand of origin with >99% accuracy.
Fast, streamlined protocol
- Go from start to finish in ~5 hours.
Large input size
- Use 100 ng-1 ¥ìg total RNA from human, mouse, or rat.
Versatility
- Get reliable data across replicates and RNA quality. Seehigh - quality, reproducible data from total RNA with a RIN (RNA IntegrityNumber) from 3-10.
Integrated with Illuminasequencing
- Generate Illumina - specificlibraries with up to 96 indexes, and retain strand information.
±×¸² 1. Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A.
Depletion of rRNA from total RNA samples with RiboGone technology.
Section B.
First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2.
Section C.
Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification.
Ç¥ 1. High-quality libraries across varying levels of RNA quality
. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.
Ç¥ 2. Sequence Alignment Metrics
. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.
Àû¿ë
Illumina-specific NGSlibrary generation that retains strand information
RNA-Seq on Illuminaplatforms for mammalian samples
Coding and non-codingtranscript information
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ±¸¼ºÇ°Àº CoA ¶Ç´Â ¸Å´º¾ó ÂüÁ¶)
- SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian Components
- Indexing Primer Set HT for Illumina - 12
- SeqAmp DNA Polymerase
Keyword :
Stranded RNA-seq
´ÜÀϼ¼Æ÷ (single cell)ÀÇ stranded RNA-Seq li..
Single cell Stranded Kit
UDI, UMI Àû¿ëÀ¸·Î ´õ¿í Ưº°ÇØÁø
[NEW] Pico Input Mammalian (v3)
Picogram RNA·ÎºÎÅÍ Strand-specific illumina ..
Pico Input Mammalian (v2)
Low Input Mammalian
High Input Strand- Specific Total RNA-Seq
HI Mammalian
Stranded RNA-Seq Kit
Total RNA-Seq Kit (FFPE, LCM »ùÇà ºÐ¼®)