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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Low Input DNA-Seq > ThruPLEX¢ç Plasma-seq Kit
cell-free DNA library preparation for Illumina NGS platforms

ThruPLEX¢ç Plasma-seq Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
R400679
ThruPLEX¢ç Plasma-Seq Kit
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24ȸ
1,457,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x102091

* º» Á¦Ç°Àº ±âÁ¸ ThruPLEX¢ç Plasma-seq Kit (Code R400490)ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°À̸ç, indexing primer kit°¡ Æ÷ÇԵǾîÀÖÁö ¾Ê½À´Ï´Ù.

  • Designed for cell-free DNA: cell-free DNA ºÐ¼®¿¡ ÃÖÀûÈ­µÈ ÇÁ·ÎÅäÄÝ°ú ½Ã¾à
  • High performance NGS libraries: high diversity with broad and reproducible GC coverage
  • Variable sample input: <1 ng to 30 ng of cell-free DNA
  • Fast and simple workflow: Æ©ºê À̵¿ ¾øÀÌ ´ÜÀÏ Æ©ºê¿¡¼­ 3 step ÇÁ·ÎÅäÄÝ·Î NGS Library Á¦Á¶ °¡´É (library Á¦ÀÛ ½Ã°£: 2½Ã°£)
  • Research applications: liquid biopsy, ctDNA¸¦ Æ÷ÇÔÇÑ ´Ù¾çÇÑ cell-free DNA »ùÇ÷κÎÅÍ ÀçÇö¼º°ú ¹Î°¨µµ ³ôÀº NGS ºÐ¼®
  • Automation-friendly: ApolloTM Library Prep System(Takara Bio USA) Beckman¢ç FXP Workstation
Á¦Ç°¼³¸í
ThruPLEX¢ç Plasma-Seq Kit´Â Ç÷Àå (Plasma) À¯·¡ÀÇ cell-free DNA·ÎºÎÅÍ ThruPLEX ±â¼úÀ» ÀÌ¿ëÇÏ¿© Illumina¢ç NGS Library¸¦ Á¦ÀÛÇÏ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°Àº ±âÁ¸ Á¦Ç°¿¡ ºñÇÏ¿© End repair, ligation ½Ã¾à ¹× ¹ÝÀÀÁ¶°ÇÀ» ÃÖÀûÈ­ÇÏ¿´À¸¸ç Plasma DNA·ÎºÎÅÍ ´Ù¾ç¼ºÀÌ º¸´Ù ³ô°í GC representationÀ» º¸À¯ÇÑ NGS Library¸¦ ±¸ÃàÇÒ ¼ö ÀÖ´Ù. Á¦ÀÛµÈ Library´Â CNV ºÐ¼®, Whole Genome Sequencing (WGS) »Ó¸¸ ¾Æ´Ï¶ó Custom panelÀ̳ª Agilent SureSelect, Roche NimbleGene SeqCap EZ¿Í °°Àº Target enrichment platform¿¡ Àû¿ë ¹× ºÐ¼®ÇÒ ¼ö ÀÖ´Ù.

º» Á¦Ç°Àº ±âÁ¸ ThruPLEX¢ç Plasma-Seq KitÀÇ ¾÷±×·¹À̵å Á¦Ç°À̸ç, Illumina index´Â Æ÷ÇÔÇÏ°í ÀÖÁö ¾Ê´Ù. ´ÙÄ«¶ó¹ÙÀÌ¿À¿¡¼­ Á¦°øÇÏ´Â ¸ðµç DNA Index Kits¸¦ Àû¿ë °¡´ÉÇÏ´Ù ( DNA Index Kit ¹Ù·Î°¡±â)


±×¸² 1. Illumina NGS workflow for cfDNA. Blood samples are collected, plasma is isolated, and cfDNA purified. ThruPLEX Plasma-Seq library preparation follows and consists of three steps: repair, ligation and amplification. Indexed libraries are purified, quantified, and pooled prior to sequencing on Illumina NGS platforms.


±×¸² 2. ThruPLEX Plasma-Seq single-tube library preparation workflow. The workflow consists of three simple steps that take place in the same PCR tube or well and eliminates the need to purify and transfer the sample material.

±×¸² 3. ThruPLEX Plasma-Seq technology. A three-step, single-tube reaction that starts with fragmented dsDNA or isolated cfDNA. Stem-loop adapters are blunt-end ligated to the repaired input DNA. These molecules are extended, then amplified using a high-fidelity polymerase to yield an indexed Illumina NGS library.
Comparison data for Cell-free DNA-seq
KAPA Hyper Prep Kit, NEBNext Ultra DNA Library Prep Kit°ú ºñ±³ÇÏ¿©, ThruPLEX¢ç Plasma-Seq KitÀÌ º¸´Ù ¶Ù¾î³­ ¼º´É°ú ÀçÇö¼º, GC Coverage¸¦ º¸À¯ÇÏ°í ÀÖÀ½À» ±Ô¸íÇÏ¿´´Ù (Technical Note: An optimized solution for cell-free DNA library preparation) (±×¸² 4, ±×¸²5).

±×¸² 4. Diverse, reproducible NGS libraries. Libraries created with the ThruPLEX Plasma-Seq Kit yielded more unique molecules (Panel A), fewer duplicate reads (Panel B), and negligible unmapped reads (Panel C). Libraries were sequenced on an Illumina NextSeq 500 as a paired-end run with 17M to 25M reads per library. Duplication rate were calculated after down-sampling the data to 17M reads per library. Representative data from each sample is shown.


±×¸² 5. Reproducible, unbiased GC coverage. The ThruPLEX Plasma-Seq Kit provided the most reproducible and unbiased GC coverage across the human genome, showing minimal variability across the nine plasma samples tested. Libraries were prepared from cfDNA isolated from an equivalent of 1ml of plasma sample and sequenced on an Illumina NextSeq 500. Four separate plasma samples were used to construct the NEBNext Ultra libraries.
Application protocol & Technical Note
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
- Template Preparation P Buffer
- Template Preparation P Enzyme
- Library Synthesis P Buffer
- Library Synthesis P Enzyme
- Library Amplification P Buffer
- Library Amplification Enzyme
- Nuclease-Free Water

Keyword :

Low Input DNA-Seq
>
All-purpose low input DNA library preparatio..
ThruPLEX¢ç DNA-seq Kit
>
cell-free DNA library preparation for Illumi..
ThruPLEX¢ç Plasma-seq Kit