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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > NGS Sample preparation > rRNA Á¦°Å½Ã¾à RiboGone¢â-Mammalian
Human, mouse, rat total RNA·ÎºÎÅÍ rRNA ¹× mtRNA¸¦ È¿°úÀûÀ¸·Î Á¦°Å

rRNA Á¦°Å½Ã¾à RiboGone¢â-Mammalian

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
634846
RiboGone-Mammalian
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
6ȸ
751,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33019
Clontech
634847
RiboGone-Mammalian
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24ȸ
2,179,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

RiboGone-Mammalian kit´Â human, mouse, ratÀÇ total RNA »ùÇ÷κÎÅÍ 5S, 5.8S, 18S, 28S nuclear rRNA (Ribosomal RNA) »Ó¸¸ ¾Æ´Ï¶ó 12S mtRNA (mitochondrial RNA)¸¦ Á¦°ÅÇÏ´Â ½Ã¾àÀÌ´Ù. ƯÈ÷, RiboGoneÀº FFPE »ùÇðú °°ÀÌ degradationµÈ »ùÇûӸ¸ ¾Æ´Ï¶ó ³·Àº qualityÀÇ RNA »ùÇÿ¡µµ Àû¿ë °¡´ÉÇÏ´Ù. RiboGone-Mammalian Kit´Â random-primed cDNA synthesis¿Í RNA-seq ¶Ç´Â transcriptom ºÐ¼® ½ÇÇè Àü, sample preparation °úÁ¤¿¡ ÀûÇÕÇÏ´Ù (including the SMARTer Stranded RNA-Seq Kit (634836), the SMARTer Universal Low Input RNA Kit for Sequencing (634938), and the SMARTer Universal Low Input RNA Library Prep Kit (634947)) (±×¸² 1).



±×¸² 1. RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10-100 ng of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).
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  • Effective rRNA removal - rRNA removal method for human, mouse and rat total RNA samples - depletes ¡Ã95% of ribosomal RNA sequences
  • Suitable for any quality RNA - designed to work with any quality starting RNA, including degraded material like FFPE samples
  • Low input RNA working range - 10 ng -100 ng starting material
  • Works seamlessly with downstream random-primed cDNA synthesis kit like the Stranded RNA-Seq Kit or SMARTer Universal Low Input RNA Library Prep Kit


±×¸² 2. Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.



±×¸² 3. Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.
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Removal of rRNA from small total RNA samples prior to random-primed cDNA synthesis
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ±¸¼ºÇ°Àº CoA ¶Ç´Â ¸Å´º¾ó ÂüÁ¶)
5X RiboGone Hyb Buffer
RNaseH (5 U/ul)
DNaseI (5 U/ul)
RNase Inhibitor (40 U/ul)
Nuclease-Free Water

RiboGone Purification Buffer

Keyword : rrna Á¦°Å,ribosomal RNA,rRNA,rrna depletion,total rna,ribozero,ribo-zero,¸®º¸°ï,depletion

NGS Sample preparation
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Human, mouse, rat total RNA·ÎºÎÅÍ rRNA ¹× mt..
rRNA Á¦°Å½Ã¾à RiboGone¢â-Mammalian
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