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50pg ChIP DNA-seq Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
R400674
ThruPLEX¢ç DNA-Seq Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24ȸ
1,357,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x102090
Clontech
R400675
ThruPLEX¢ç DNA-Seq Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
48ȸ
2,618,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
R400676
ThruPLEX¢ç DNA-Seq Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96ȸ
4,073,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
R400677
ThruPLEX¢ç DNA-Seq Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
480ȸ (R400676 x 5)
18,329,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â


* º» Á¦Ç°Àº ±âÁ¸ ThruPLEX¢ç DNA-seq Kit (Code R400523)ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°À̸ç, indexing primer kit°¡ Æ÷ÇԵǾîÀÖÁö ¾Ê½À´Ï´Ù.
  • High performance from low inputs - ¹Ì·®ÀÇ DNA »ùÇà (50 pg ~ 50 ng)·ÎºÎÅÍ Illumina¢ç NGS library Á¦ÀÛ
  • Fast and simple workflow - Æ©ºê À̵¿ ¾øÀÌ ´ÜÀÏÆ©ºê (single-tube or single-well)¿¡¼­ ¼¼ ¹øÀÇ ½ÇÇè °úÁ¤ (3 step)À¸·Î Á¦ÀÛ ¿Ï·á (library Á¦ÀÛ ½Ã°£ : ¾à 2½Ã°£)
  • Compatible with major target enrichment platforms - ÁÖ¿ä Target Enrichment Ç÷§Æû¿¡ Àû¿ë °¡´É - Agilent SureSelect¢ç, Roche Nimblegen¢ç SeqCap¢ç EZ, and IDT xGen¢ç Lockdown¢ç probes ¿Ü
    ¡Ø DNA Target Enrichment Protocol ÀÚ¼¼È÷ º¸±â
Á¦Ç°¼³¸í
ThruPLEX¢ç DNA-seq KitÀº ThruPLEX¢ç ±â¼úÀ» ÀÌ¿ëÇÏ¿© ±Ø¼Ò·®ÀÇ DNA·ÎºÎÅÍ Illumina¢ç NGS Library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇÏ¸é ºÒ°ú 50 pg ¼öÁØÀÇ ±Ø¼Ò·® DNA·Î ÃÖ´ë 384 multiplexing¿¡ ´ëÀÀ °¡´ÉÇÑ Illumina¢ç NGS Library¸¦ Á¦ÀÛ °¡´ÉÇÏ´Ù. º» Á¦Ç°Àº »ùÇà Á¾·ù¿¡ »ó°ü¾øÀÌ fragmentationµÈ DNA¶ó¸é ¸ðµÎ Àû¿ëÀÌ °¡´ÉÇÏ´Ù (Genomic, biofluids such as cell-free DNA, DNA from FFPE materials, cDNA).
ThruPLEX¢ç DNA-seq KitÀ» ÀÌ¿ëÇÏ¸é ´ÜÀÏÆ©ºê¿¡¼­ Æ©ºê À̵¿ ¾øÀÌ 2½Ã°£ ¸¸¿¡ NGS Library¸¦ Á¦ÀÛ °¡´ÉÇÏ´Ù. ThruPLEX¢ç DNA-seq KitÀº ´Ù¾çÇÑ DNA-seq application°ú RNA-seqÀ» À§ÇÑ cDNA ±×¸®°í ChIP-seq µî¿¡ »ç¿ëµÉ ¼ö ÀÖÀ¸¸ç, ÁÖ¿ä target enrichment platform protocolµµ ÇÔ²² Á¦°øµÇ°í ÀÖ´Ù (¡Ø DNA Target Enrichment Protocol ÀÚ¼¼È÷ º¸±â).

º» Á¦Ç°Àº ±âÁ¸ ThruPLEX¢ç DNA-Seq KitÀÇ ¾÷±×·¹À̵å Á¦Ç°À̸ç, Illumina index´Â Æ÷ÇÔÇÏ°í ÀÖÁö ¾Ê´Ù. ´ÙÄ«¶ó¹ÙÀÌ¿À¿¡¼­ Á¦°øÇÏ´Â ¸ðµç DNA Index Kits¸¦ Àû¿ë °¡´ÉÇÏ´Ù ( DNA Index Kit ¹Ù·Î°¡±â)


±×¸² 1. ThruPLEX DNA-Seq single-tube library preparation workflow. The ThruPLEX DNA-Seq workflow consists of three simple steps that take place in the same PCR tube or well, eliminating the need to purify and transfer the sample material.)


±×¸² 2. ThruPLEX¢ç  ±â¼ú (ÀÚ¼¼È÷ º¸±â)
Step 1. ´ÜÆíÈ­µÈ double stranded DNA ¸»´ÜÀ» °íÈ¿À²·Î RepairÇÑ´Ù
Step 2. DNA ÀýÆíÀÇ 5¡¯ ¸»´Ü¿¡ Stem-Loop adapter°¡ ligationµÇ¸ç, 3¡¯ ¸»´Ü¿¡´Â nickÀ» ³²±ä´Ù. ÀÌÈÄ extension °úÁ¤À» ÅëÇØ Stem-Loop adapterÀÇ ÀϺΰ¡ Á¦°ÅµÈ´Ù.
Stem-Loop adapter´Â ³ëÃâµÈ ´ÜÀÏ°¡´ÚÀÌ ¾ø°í, 5¡¯ ¸»´ÜÀÌ blockµÇ¾î ÀÖ¾î adapter°£ÀÇ dimer Çü¼ºÀÌ ¾ïÁ¦µÉ »Ó¸¸ ¾Æ´Ï¶ó ¼­¿­ ºÐ¼®¿¡¼­ÀÇ background¸¦ °¨¼Ò½Ãų ¼ö ÀÖ´Ù.
Step 3. Illumina¢ç index°¡ Æ÷ÇÔµÈ primer·Î ÁõÆøÇÏ¿© Illumina¢ç NGS ºÐ¼®¿ë Llibrary Á¦ÀÛÀ» ¿Ï·áÇÑ´Ù.

Ç¥ 1. DNA Sample Requirements for ThruPLEX¢ç DNA-seq Kit
DNA Sample Requirements

Nucleic Acid

Fragmented double-stranded DNA or cDNA

Source

Cells, plasma, urine, other biofluids, FFPE, tissues, fresh tissues, frozen tissues

Type

Mechanically sheared; enzymatically fragmented;
ChIP-DNA; low-molecular weight cell-free DNA

Molecular Weight

< 1,000 bp

Input Amount

50 pg - 50 ng

Input Volume

10 §¡


* ÀÚ¼¼ÇÑ ³»¿ëÀº »ç¿ëÀÚ ¸Å´º¾óÀ» Âü°íÇϼ¼¿ä.
Application
* ¸µÅ©¸¦ Ŭ¸¯ÇϽøé ThruPLEX¢ç DNA-seq KitÀÇ ´Ù¾çÇÑ ½ÇÇè µ¥ÀÌÅ͸¦ È®ÀÎÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.
Application

Technical Note

ChIP-Seq

Low cell number ChIP-seq using the ThruPLEX DNA-seq kit as a tool for epigenetic profiling

FFPE DNA

A streamlined solution for generating high-quality NGS libraries from FFPE DNA samples

Low input exome-seq

Low-input whole-exome sequencing using ThruPLEX technology and Agilent SureSelect Target Enrichment Systems

Low volume DNA shearing

Using Covaris microTUBE-15 to shear samples for ThruPLEX library preparation

±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
ThruPLEX¢ç DNA-seq Kit
  • Template Preparation D Buffer
  • Template Preparation D Enzyme
  • Library Synthesis D Buffer
  • Library Synthesis D Enzyme
  • Library Amplification D Buffer
  • Library Amplification Enzyme
  • Nuclease-Free Water

Keyword :

ChIP-Seq / Meth-Seq
>
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Meth-Seq DNA Enrichment Kit