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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Stranded RNA-seq > Mid Input Stranded RNA-Seq Kit

Mid Input Stranded RNA-Seq Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
634838
SMARTer¢ç Stranded RNA-Seq Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
48 ȸ
(Package)
6,294,000¿ø  Àç°í ¼ÒÁø ÈÄ Á¾¸Å Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33058
Clontech
634839
SMARTer¢ç Stranded RNA-Seq Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 ȸ
(Package)
8,736,000¿ø  Àç°í ¼ÒÁø ÈÄ Á¾¸Å Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
634862
SMARTer¢ç Stranded RNA-Seq Kit HT
°ü·ÃÇмú ±¸¸ÅÇϱâ
96ȸ
(Package)
8,736,000¿ø  Àç°í ¼ÒÁø ÈÄ Á¾¸Å Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33057, x33058

¡Ø ¾È³»
º» Á¦Ç°Àº SMART-Seq¢ç Total RNA Mid Input (Code 635048, 635049, 635050) Á¦Ç°À¸·Î ¸®´º¾óµÇ¾ú½À´Ï´Ù.
ÀÚ¼¼ÇÑ ³»¿ëÀº ´ÙÄ«¶óÄÚ¸®¾Æ (02-2081-2510)·Î ¹®ÀÇ ÁÖ¼¼¿ä.

  • SMART¹ýÀ» ÀÌ¿ëÇÑ NGS¿ë Strand-specific NGS Library Á¦ÀÛ
  • 100pg-100ngÀÇ RNA (purified Poly A+ RNA ¶Ç´Â rRNA depleted RNA)¿¡¼­ Library Á¦ÀÛ
  • Illumina¢ç sequencer¿¡ ÀûÇÕÇÑ index¿Í adaptor Àû¿ë
  • 4½Ã°£ À̳»¿¡ RNA-seq library Á¦ÀÛ °¡´É
  • GC-, AT-rich template¿¡ ÃÖÀûÀÎ PCRÈ¿¼Ò Æ÷ÇÔ
Á¦Ç°¼³¸í
SMARTer¢ç Stranded RNA-Seq Kit´Â 100pg~10ngÀÇ RNA¸¦ ÀÌ¿ëÇÏ¿© Àü»çüÀÇ strand informationÀ» À¯ÁöÇϸ鼭 Illumina¢ç sequencing platforms¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Â library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â ½Ã¾àÀÌ´Ù. º» Á¦Ç°Àº random primer ¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© polyA+ RNA¿Í rRNA-removed total RNA·ÎºÎÅÍ NGS¿ë cDNA library¸¦ Á¦ÀÛÇϸç, mammalian RNA (poly A+ RNA)¿Í ¹ÚÅ׸®¾Æ »ùÇà (non-poly A+ RNA)¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
±âÁ¸ÀÇ Clonetch SMARTerÀÇ ¿ø¸®¸¦ ÀϺΠº¯ÇüÇÏ¿© ½ÇÇè °úÁ¤ Áß¿¡, Illumina¢ç index¿Í adaptor¸¦ °áÇÕÇϱ⠶§¹®¿¡ º°µµÀÇ Library preparation kit°¡ ÇÊ¿äÇÏÁö ¾ÊÀ¸¸ç, ºÒ°ú 4½Ã°£ ¸¸¿¡ Illuimina¢ç sequencing¿¡ Àû¿ë °¡´ÉÇÑ indexed library Á¦ÀÛÀÌ °¡´ÉÇÏ´Ù.
SMARTer¢ç Stranded RNA-Seq HT (Code 634862)´Â 8Á¾·ùÀÇ Forward primer¿Í 12Á¾·ùÀÇ Reverse primer¸¦ Æ÷ÇÔÇÏ°í ÀÖ¾î ÃÖ´ë 96°³ÀÇ sampleÀ» ºÐ¼®ÇÒ ¼ö ÀÖ´Â High-throughput systemÀÌ´Ù.


¡Ø SMARTer¢ç Stranded RNA-Seq Kit ¶Ç´Â SMARTer¢ç Stranded RNA-Seq Kit HT Á¦Ç°À» ÀÌ¿ëÇÒ °æ¿ì, ribosomal RNA (rRNA) Á¦°Å (rRNA depletion)ÀÌ ÇÊ¿äÇϸç, ClontehÀÇ RiboGone¢â-Mammalian (Code 634846) Á¦Ç°ÀÇ »ç¿ëÀ» ÃßõÇÑ´Ù


±×¸² 1. NGS Work flow for the SMARTer¢ç Stranded RNA-Seq Kit


±×¸² 2. ¼Ò·®ÀÇ RNA (100ng ¶Ç´Â 100pg)·ÎºÎÅÍ ÀçÇö¼º ³ôÀº RNA-Seq µ¥ÀÌÅ͸¦ ¾òÀ» ¼ö ÀÖ¾ú´Ù.


±×¸² 3. Indexed cDNA libraries were prepared according to the SMARTer Stranded RNA-Seq Kit protocol using twelve indices, and sequenced on an Illumina¢ç HiSeq¢ç Platform with 2 x 100 bp paired end reads. Short, overlapping reads originating from different strands of the genomic DNA were distinguished from each other, enabling quantitative expression analysis and accurate genome annotation. 99% of RNA-Seq reads mapped to the correct strand.


Ç¥ 1. Even at the lowest levels tested (100 pg polyA+ input RNA), the SMARTer¢ç Stranded RNA-Seq Kit was able to maintain high accuracy while detecting ~15,000 genes using strand-specific alignments.
Applications
  • Illumina¢ç-specific NGS library production that retains strand information.
  • RNA-Seq on Illumina¢ç platforms for both mammalian and prokaryotic samples.
  • Coding and non-coding transcript information
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ±¸¼ºÇ°Àº CoA ¶Ç´Â ¸Å´º¾ó ÂüÁ¶)
SMARTer¢ç Stranded RNA-Seq Kit(Code 634836 ¿Ü)
* SMARTer¢ç Stranded RNA-Seq Components
* llumina¢ç Indexing Primer Set
* SeqAmp¢â DNA Polymerase

SMARTer¢ç Stranded RNA-Seq Kit HT (Code 634862)
* SMARTer¢ç Stranded RNA-Seq Components
* Indexing Primer Set HT for Illumina¢ç
* SeqAmp¢â DNA Polymerase
°ü·Ã Á¦Ç°
- Input sample ¾ç¿¡ µû¶ó ¾Æ·¡ÀÇ Á¦Ç°À» ÃßõÇÕ´Ï´Ù.
- ¾Æ·¡ÀÇ Á¦Ç°Àº ¼Ò·®ÀÇ total RNA (10-100ng)·ÎºÎÅÍ rRNA¸¦ Á¦°ÅÇÏ´Â RiboGone¢â-Mammalian (Code 634846)ÀÌ Á¦Ç° ³»¿¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù.

Input sample

 

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