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cDNA ÇÕ¼º
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Reverse Transcription (Takara)
> Reverse Transcriptase XL (AMV) for RT-PCR Kit
RNA PCR KitÀÇ Component
Reverse Transcriptase XL (AMV) for RT-PCR Kit
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Product index
Reverse Transcription (Tak..
High fidelity RTase, P..
High Fidelity, Full Le..
PrimeScript¢â IV 1st s..
PrimeScript¢â II 1st s..
PrimeScript¢â Reverse ..
PrimeScript¢â 1st stra..
Reverse Transcriptase ..
Recombinant RNase Inhi..
Reverse Transcriptase ..
Ribonuclease Inhibitor
cDNA Library Construct..
¿ªÀü»ç ¹ÝÀÀ¿ë Primer (..
Double strand cDNA ÇÕ..
Oligo(dT) 15 primer
Reverse Transcription (Clo..
[°³¿ä] So Easy Product
µ¿°á°ÇÁ¶Å¸ÀÔ RT Premix
MMLV Reverse Transcrip..
SMARTScribe Reverse Tr..
Advantage RT-for-PCR K..
SMART MMLV Reverse Tra..
TITANIUM One-Step RT-P..
SMART¢ç Full length cDNA ..
[°³¿ä] SMARTer¢ç cDNA ..
RACE 5/3 Kit (Unknown ..
Full length cDNA Synth..
Marathon cDNA Amplific..
cDNA Library (Human, Mouse..
Human cDNA Library
Rat cDNA Library
Premade cDNA
Á¶Á÷ ¹× ¼¼Æ÷ÁÖ À¯·¡ÀÇ ..
QUICK-Clone cDNA (Huma..
QUICK-Clone cDNA (Mous..
QUICK-Clone cDNA (Rat)
RACE ¿ë Marathon-Ready..
RACE ¿ë Marathon-Ready..
RACE ¿ë Marathon-Ready..
Total RNA£¦Poly A RNA
Human Tumor, Human Cel..
Premium Poly A+ RNA (H..
Premium Poly A+ RNA (H..
Premium Total RNA (Hum..
Premium Total RNA (Mou..
Premium Total RNA (Rat..
qPCR Human Reference c..
Total RNA Master Panel
Universal Reference To..
±× ¿Ü °ü·ÃÁ¦Ç° (RNA)
RNase-free Water
RNase-OFF¢ç
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10 mM
Potassium Phosphate (pH7.2)
0.1 mM
EDTA
2 mM
DTT
0.2 %
Triton X-100
50 %
Glycerol
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-80¡É µ¿°áº¸Á¸ (µ¿°áÀ¶ÇØÀÇ ¹Ýº¹¿¡ µû¸¥ È°¼º ÀúÇÏ´Â ¾øÀ¸³ª °¡±ÞÀû ÇÇÇÒ °Í)
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º» Á¦Ç°Àº Avian Myeroblastosis Virus (AMV)·ÎºÎÅÍ °íµµ·Î Á¤Á¦ÇÑ °ÍÀÌ´Ù.
TaKaRa¿¡¼ ÆǸÅÇÏ°í ÀÖ´Â RNA PCR Kit AMV RTase¿Í °°Àº ³óµµ (5 U/§¡)·Î Á¶Á¤ÇÑ °ÍÀÌ´Ù. RNA (PolyA-tailed RNA Ladder(1.4~9.5 kb)¸¦ ÁÖÇüÀ¸·Î ÇÏ´Â cDNAÇÕ¼º¹ÝÀÀ°è ÁÖÇüÀÇ 40~70%°¡ ¿ªÀü»çµÇ¾î ±× ÁßÀÇ 55~80%°¡ full length cDNA¸¦ ÇÕ¼ºÇÏ´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù.
¶Ç º» Á¦Ç°Àº ¾Æ·¡ Ç¥±âÇÑ Á¦Ç°¿¡ Æ÷ÇÔµÈ AMV Reverse Transcriptase XL°ú µ¿ÀÏÇÏ´Ù.
- TaKaRa RNA PCR Kit (AMV) Ver. 2.1(TaKaRa Code RR019A/B)
- TaKaRa RNA LA PCR Kit (AMV) Ver.1.1(TaKaRa Code RR012A)
- TaKaRa One Step RNA PCR Kit (AMV) (TaKaRa Code RR024A/B)
- mRNA Selective PCR Kit Ver.1.1(TaKaRa Code RR025A/B)
- 3¡¯- Full RACE Core Set (TaKaRa Code 6121)
- 5¡¯- Full RACE Core Set (TaKaRa Code 6122)
- Enzyme Set-FDD (TaKaRa Code R025A/B)
- Enzyme Set-DD (TaKaRa Code R024A/B)
È°¼ºÀÇ Á¤ÀÇ
Poly (rA)¡¤ oligo (dT)¸¦ °¢°¢ ÁÖÇü°ú ÇÁ¶óÀ̸ӷΠ37¡É¿¡¼, 10ºÐ µ¿¾È 1 nmolÀÇ [
3
H]dTMP¸¦ »êºÒ¿ë¼º ħÀü¹°¿¡ ³Ö´Â È°¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
È°¼ºÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º
50 mM
Tris-HCl (pH8.3)
40 mM
KCl
6 mM
MgCl
2
4 mM
DTT
0.4 mM
Poly(rA)¡¤ oligo(dT)
12-18
0.5 mM
[
3
H]dTTP
Ç°Áú°ËÁ¤
Reverse Transcriptase XL (AMV)(TaKaRa Code 2620A)°ú µ¿ÀÏÇÏ´Ù.
Keyword :
Reverse Transcription (Takara)
High fidelity RTase, PrimeScript¢â
High Fidelity, Full Length cDNA ÇÕ¼º °ü·ÃÁ¦Ç°
Premix ŸÀÔÀÇ cDNA ÇÕ¼º ½Ã¾à
PrimeScript¢â IV 1st strand cDNA Synthesis Mix
¾Ç¼¼»ç¸® ´Ü¹éÁúÀÌ Ãß°¡ µÈ ÃÖ»ó±Þ Full-length..
PrimeScript¢â II 1st strand cDNA Synthesis Kit
Ź¿ùÇÑ ½ÅÀå´É·ÂÀ» °¡Áø ½Å°³³ä ¿ªÀü»ç È¿¼ÒÀÇ ..
PrimeScript¢â Reverse Transcriptase
PrimeScript RTase¸¦ ÀÌ¿ëÇÑ 1st strand cDNA ..
PrimeScript¢â 1st strand cDNA Synthesis Kit
Reverse Transcriptase XL (AMV)
RT-PCR ÇʼöÇ°
Recombinant RNase Inhibitor
RNA PCR KitÀÇ Component
Reverse Transcriptase XL (AMV) for RT-PCR Kit
Ribonuclease Inhibitor
cDNA Library ±¸Ãà All-in-One Kit
cDNA Library Construction Kit
¿ªÀü»ç ¹ÝÀÀ¿ë Primer (Random Primer)
Double strand cDNA ÇÕ¼º
¿ªÀü»ç¹ÝÀÀ¿ë primer
Oligo(dT) 15 primer