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SimplyLoad DNA ladder
DNA Markers
Lonza DNA Marker´Â PCR »ê¹°À̳ª Á¦ÇÑÈ¿¼Ò ¹ÝÀÀ¹°ÀÇ ºü¸¥ »çÀÌÁî È®ÀÎÀ» À§ÇÑ Á¦Ç°À¸·Î ethidium bromide (EtBr)
stainingÀ» ÅëÇØ °£ÆíÇÏ°Ô È®ÀÎ °¡´ÉÇÏ´Ù.
- 1 kb DNA Marker (1 kb - 10 kb)
1, 1.5, 2, 2.5, 3, 4, 5, 7, 10 kb·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
- 50 bp DNA Marker (50 bp - 2,500 bp)
50, 100, 200, 300, 400, 500, 525, 700, 1,000, 1250, 1500, 2000, 2500 bp ·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
- 50 bp DNA Marker (50 bp - 1,000 bp)
50, 100, 200, 300, 400, 500, 525, 700, 1,000 bp ·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
DNA Markers ¸ð½Äµµ
DNA Ladders
Lonza DNA Ladder´Â ºü¸£°í ½±°Ô DNA sample»çÀÌÁ È®ÀÎ ÇÒ ¼ö ÀÖ´Â Á¦Ç°À¸·Î ¼±¸íÇÑ Çػ󵵸¦ °¡Áö°í ÀÖ´Ù.
- 20 bp DNA Ladder (20 bp - 500 bp)
20 bp °£°ÝÀ¸·Î ÃÑ 25°³ÀÇ fragments·Î ÀÌ·ç¾îÁ® ÀÖÀ¸¸ç 20-500 bp±îÁö sample »çÀÌÁî È®ÀÎ °¡´ÉÇÑ Á¦Ç°ÀÌ´Ù.
- 20 bp Extended Range DNA Ladder (20 bp - 1,000 bp)
20 bp °£°ÝÀ¸·Î ÃÑ 50°³ÀÇ fragments·Î ÀÌ·ç¾îÁ® ÀÖÀ¸¸ç 20-1,000 bp±îÁö sample »çÀÌÁî È®ÀÎ °¡´ÉÇÑ Á¦Ç°ÀÌ´Ù.
- 100 bp DNA Ladder (100 bp - 1,000 bp)
100 bp °£°ÝÀ¸·Î ÃÑ 10°³ÀÇ fragments·Î ÀÌ·ç¾îÁ® ÀÖÀ¸¸ç 100-1,000 bp±îÁö sample »çÀÌÁî È®ÀÎ °¡´ÉÇÑ Á¦Ç°ÀÌ´Ù.
- 100 bp Extended Range DNA Ladder (100 bp - 3,000 bp)
100 bp °£°ÝÀ¸·Î ÃÑ 30°³ÀÇ fragments·Î ÀÌ·ç¾îÁ® ÀÖÀ¸¸ç 100-3,000 bp±îÁö sample »çÀÌÁî È®ÀÎ °¡´ÉÇÑ Á¦Ç°ÀÌ´Ù.
- 500 bp DNA Ladder (500 bp - 8,000 bp)
500 bp °£°ÝÀ¸·Î ÃÑ 16°³ÀÇ fragments·Î ÀÌ·ç¾îÁ® ÀÖÀ¸¸ç 500-8,000 bp±îÁö sample »çÀÌÁî È®ÀÎ °¡´ÉÇÑ Á¦Ç°ÀÌ´Ù.
- DNA QuantLadder (100 bp - 1,000 bp), DNA Reverse QuantLadder (100 bp - 1,000 bp)
100-1.000 bpÀÇ ¹êµåÀÇ ³óµµ¸¦ È®ÀÎ °¡´ÉÇÑ Á¦Ç°ÀÌ´Ù (°¢ ¹êµåº° ³óµµ ¸ð½Äµµ Âü°í).
DNA Ladders ¸ð½Äµµ
Á¦Ç° »ç¿ë
Á¦Ç°¸í |
Ladder |
6X Loading Buffer |
Distilled Water |
ºñ°í |
1 kb DNA Marker (1 kb - 10 kb) |
5.0 ¥ìl |
1.0 ¥ìl |
- |
For a 6 ¥ìl total load volume |
50 bp DNA Marker (50 bp - 2,500 bp) |
5.0 ¥ìl |
1.0 ¥ìl |
- |
50 bp DNA Marker (50 bp - 1,000 bp) |
5.0 ¥ìl |
1.0 ¥ìl |
- |
20 bp DNA Ladder (20 bp - 500 bp) |
1.0 ¥ìl |
2.5 ¥ìl |
11.5 ¥ìl |
For a 15 ¥ìl total load volume |
20 bp Extended Range DNA Ladder (20 bp - 1,000 bp) |
1.5 ¥ìl |
2.5 ¥ìl |
11.0 ¥ìl |
100 bp DNA Ladder (100 bp - 1,000 bp) |
1.6 ¥ìl |
2.5 ¥ìl |
10.9 ¥ìl |
100 bp Extended Range DNA Ladder (100 bp - 3,000 bp) |
1.5 ¥ìl |
2.5 ¥ìl |
11.0 ¥ìl |
500 bp DNA Ladder (500 bp - 8,000 bp) |
1.5 ¥ìl |
2.5 ¥ìl |
11.0 ¥ìl |
DNA QuantLadder (100 bp - 1,000 bp) |
2.5 ¥ìl |
2.5 ¥ìl |
10.0 ¥ìl |
DNA Reverse QuantLadder (100 bp - 1,000 bp) |
2.5 ¥ìl |
2.5 ¥ìl |
10.0 ¥ìl |
°ü·Ã Á¦Ç°
»ç¿ëÀÌ °£ÆíÇÑ Lonza AccuGene Buffer ½Ã¸®Áî ¹Ù·Î°¡±â
´Ù¾çÇÑ ½ÇÇè¿¡ Àû¿ë °¡´ÉÇÑ º£½ºÆ®¼¿·¯ SeaKem Agarose ¹Ù·Î°¡±â
EtBrº¸´Ù ³ôÀº °¨µµ·Î staining °¡´ÉÇÑ GelStar Nucleic Acid Stain ¹Ù·Î°¡±â