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Tet-Off Cell Lines

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Clontech
631152
HEK 293 Tet-Off Advanced Cell Line
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°¢ 1 ml
(Package)
1,725,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33147, x33149
Clontech
631156
HeLa Tet-Off Advanced Cell Line
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°¢ 1 ml
(Package)
1,725,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33147, x33149

[ÁÖÀÇ] µ¿°áµÈ ¼¼Æ÷ÁÖÀÇ Çص¿(Thawing) ½Ã, ¼¼Æ÷ »ýÁ¸À² Çâ»óÀ» À§ÇÏ¿© collagen-coated plate ¶Ç´Â flaks¸¦ »ç¿ëÇϽʽÿÀ.
       ÃßÈÄ ¼¼Æ÷ ¹è¾ç ½Ã¿¡µµ ¼¼Æ÷ÁÖÀÇ ºÎÂøÀ²(adherence)ÀÌ ÁÁÁö ¾Ê´Ù¸é collagen-coated plate ¶Ç´Â flask¸¦ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.

NOTE: We recommend using collagen-coated plates or flasks for efficient culturing of frozen stocks. Vessels coated with compounds other than collagen may also provide suitable growth substrates (e.g. poly-L-lysine), but only collagen-coated plates (e.g. BD BioCoat Cellware, Collagen Type I) have been tested at Clontech. Once recovered, the cells may be cultured directly on tissue culture plastic. However, if adherence is poor, we recommend using only collagen-coated vessels.

Tet-Off and Tet-Off Advanced inducible cell lines are host cell lines that greatly simplify the task of setting up tetracycline-inducible gene expression systems which are activated by removing doxycycline (Dox) from the culture medium (Figure 1; 1, 2). Our Tet-On¢ç Advanced and Tet-On inducible cell lines are also available, which are activated by adding Dox to the culture medium.
These rigorously tested clonal cell lines:
  • Stably express either the second-generation Tet-Off Advanced transactivator protein or the original Tet-Off transactivator protein
  • Allow you to induce high-level expression of your gene-of-interest by removing (Dox) from the culture of transfected cells (Figure 2)
  • Can save you up to two months of time in developing a complete Tet-controlled system.

Easily create an inducible system by first placing your gene under control of a tetracycline-responsive promoter (e.g. PTight in pTRE-Tight), transfecting it into one of these G418-resistant cell lines, and selecting for double-stable clones.

Our PC12 Tet-Off Cell Line responds normally to nerve growth factor (NGF). For more details, see our report Neural Differentiation in Tet-Inducible PC12 Cell Lines.

References
1. Gossen, M. & Bujard, H. (1992) Proc. Natl. Acad. Sci. USA 89:5547-5551.
2. Gossen, M., et al. (1995) Science 268:1766-1769.
Components
  • Tet-Off or Tet-Off Advanced Cell Line
  • CHO AA8-Luc Tet-Off Control Cell Line
  • Tet System Approved FBS (50 ml)
Storage Conditions
196¡ÆC
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)

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