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Home > 전제품보기 > 항체 · ELISA · Signal > OsteoㆍAdipo assay > PPiLight Inorganic Pyrophosphate Assay

PPiLight Inorganic Pyrophosphate Assay

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제조사 제품코드 제품명 용량 가격
(부가세별도)		 비고 사용자매뉴얼
Lonza
 LT07-610
 PPiLight Inorganic Pyrophosphate Assay
관련학술 구매하기 		500 tests
 가격문의   Lonza 시약 전품목 20% 할인 PPiLight_inorganic_pyrophosphate_assay.pdf

제품설명
Inorganic pyrophosphate (also referred to as diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number of different enzymatic reactions. Enzymes that utilize PPi as a substrate may include phosphotransferases (EC 2.7) and pyrophosphatases (EC 3.6.1.1). Enzymes that generate PPi are more numerous and may include cyclases (EC 4.6.1), hydrolyses (EC 3) and ligases (EC 6).

The PPiLight™ Assay is a non-radioactive bioluminescent assay for the detection of inorganic pyrophosphate. In the presence of PPi the detection reagent catalyses the conversion of AMP to ATP. The assay uses luciferase, which produces light from the newly formed ATP and luciferin.


Figure 1: Bioluminescent reaction
특장점
  • Fast - Measure enzyme activity via pyrophosphate consumption in 1 hour
  • Simple - The 2 step, luminescent assay doesn’t require radioactive substrates, beads, detection antibodies or modified substrates
  • Wide Detection Range - Sensitive to 0.02 μM with a linear range to 10 μM
  • Versatile - Scalable to 96-, 384-, 1586-well formats
Applications
Measure activity of:
  • Phosphotransferases
  • Pyrophosphotases
  • Cyclases
  • Ligases
  • Hydrolases
  • DNA Polymerases

보존
2°C - 8°C

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