¿À´Ã ÇÏ·ç ±×¸¸º¸±â
´Ý±â
·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
|
Á¦Ç°»ó´ã¹®ÀÇ
°í°´Áö¿ø
¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
02-2081-2510
Email ¦¢
support@takara.co.kr
´ëÀüÁö»ç
¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
042-828-6525
Email ¦¢
tkbd@takara.co.kr
¾÷¹«½Ã°£¾È³»
[ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Á¦Ç°Á¤º¸
°øÁö
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
Learning Center
ȸ»çÁ¤º¸
ÀüÁ¦Ç°º¸±â
Á¦Ç°¼±Åð¡À̵å
Custom Service
À̺¥Æ®/ÇÁ·Î¸ð¼Ç
´ÙÄ«¶ó¼Ò½Ä
Takara Bio°ø½Ã
½ÅÁ¦Ç°¾È³»
¶óÀ̼±½º¾È³»
±â¼úÁö¿ø¹®ÀÇ
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
LMO¾È³»
ºê·Î¼Å
On-line tool
eNews
樨毢
CoA & MSDS
°³¿ä & ¸®½ºÆ®
¼Ò°³
µµÀԺ귣µå Á¤º¸
´ë¸®Á¡Á¤º¸
ã¾Æ¿À½Ã´Â±æ
Home
>
ÀüÁ¦Ç°º¸±â
>
Epigenetics
>
Chromatin ¸é¿ª ħ°¨ (ChIP)
> EpiScope¢ç Nucleosome Preparation Kit
Genome»óÀÇ Nucleosome À§Ä¡ ºÐ¼®
EpiScope¢ç Nucleosome Preparation Kit
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Takara
5333
EpiScope¢ç Nucleosome Preparation Kit
50 ȸ
(Package)
424,800¿ø
531,000¿ø
09.02 ~ 10.31
Product index
Overview
[¼±Åð¡À̵å] Epigeneti..
[°³¿ä] ChIP-Seq Librar..
Cancer ¿¬±¸ °¡À̵å
DNA Methlyation ¿¬±¸
Metly-sensitive/non-se..
TaKaRa EpiTaq HS (for ..
EpiScope¢ç MSP Kit
TB Green¢ç Premix Ex T..
¸ÞÆ¿È/ºñ¸ÞÆ¿È ÄÁÆ®·Ñ..
McrBC
Methylated DNA ºÐ¸®¿Í ºÐ¼®
EpiXplore Methylated D..
Meth-Seq DNA Enrichmen..
Chromatin ¸é¿ª ħ°¨ (ChIP)
Micrococcal Nuclease
Anti-Histone monoclona..
EpiXplore ChIP Assay K..
DNA Fragmentation Kit
ChIP Elute Kit
EpiScope¢ç Nucleosome ..
Capturem¢â IP £¦ Co-IP..
ChIP-seq, Cut&Run-seq (NGS..
[Àû¿ë] ¼Ò·®¼¼Æ÷·ÎºÎÅÍ ..
[Customer Spotlight] C..
[DNA-Seq ±â¼ú°³¿ä] Thr..
[ChIP-seq ³í¹®¸®½ºÆ®] ..
SMARTer¢ç ThruPLEX¢ç D..
DNA SMART ChIP-Seq Kit
SMARTer¢ç DNA index ki..
miRNA ¹ßÇö ¡¤ Á¤·®
[Cancer Research] Canc..
MicroRNA ¿¬±¸¿ë Á¦Ç° ..
MicroRNA qRT-PCR Á¤·®
Tet Inducible miRNA ¹ß..
Small RNA-Seq Library ..
MicroRNA-Seq Library P..
miRNA ¹ßÇö vector (Çü..
Epigenetics¸¦ À§ÇÑ Cloning..
Online In-Fusion Tools
Mighty TA-cloning Kit
Nucleosome¿¡ °¨°Ü ÀÖ´Â DNA ȸ¼ö
°Ô³ð DNA¿¡¼ Nucleosome À§Ä¡ ºÐ¼®¿¡ °¡´É
0.5~2¡¿10
6
°³ ¼¼Æ÷¿¡¼ ¾à 90ºÐ¸¸¿¡ Á¶Á¦ °¡´É
Á¦Ç°¼³¸í
º» Á¦Ç°Àº µ¿¹° ¹è¾ç¼¼Æ÷·ÎºÎÅÍ nucleosomeÀ» ¸¸µé±â À§ÇÑ KitÀÌ´Ù. ¸¸µé¾îÁø nucleosome¿¡¼ ÃßÃâÇÑ DNA¸¦ ÀÌ¿ëÇÏ¿© Real Time PCR, °í¼Ó ½ÃÄö½º ºÐ¼®À» ÅëÇÏ¿© °Ô³ð»ó¿¡¼ÀÇ nucleosome À§Ä¡¸¦ ºÐ¼®ÇÒ ¼ö ÀÖ´Ù.
½ÇÇè°³¿ä
1. ¼¼Æ÷ÀÇ ¿ëÇØ¿Í ÇÙÀÇ ¿ø½É ħ°
2. È÷½ºÅæ¿¡ °¨°ÜÀÖÁö ¾ÊÀº DNA¸¦ Micrococcal Nuclease¸¦ ÀÌ¿ëÇÏ¿© Àý´Ü, Á¦°Å
3. Nucleosome¿¡ Proteinase K¸¦ ó¸®ÇÏ¿© È÷½ºÅæÀ» Á¦°Å
4. DNAȸ¼ö
(º°µµÀÇ NucleoSpin Extract II¸¦ ÀÌ¿ëÇÏ¿© DNAÁ¤Á¦)
ȸ¼öµÈ DNA´Â nucleosomeÀ» Çü¼ºÇÏ°í ÀÖ´ø °Ô³ð DNA ¿µ¿ª¿¡ ÇØ´çµÈ´Ù. È÷½ºÅæ¿¡ °¨°Ü ÀÖÁö ¾ÊÀº °Ô³ð DNA´Â Micrococcal Nuclease¿¡ ÀÇÇØ Àý´ÜµÇ¾î Á¦°ÅµÈ´Ù.
[½ÇÇè°³¿ä]
³»¿ë
(50ȸºÐ)
1. Cytoplasmic Lysis Buffer
25 ml ¡¿ 2°³
2. Protease Inhibitor Cocktail (100 ¡¿)
550 ¥ìl
3. Micrococcal Nuclease (20 units/¥ìl)
50 ¥ìl
4. Micrococcal Nuclease Buffer (10¡¿ )
1 ml
5. RNase A (20 mg/ml)
100 ¥ìl
6. 0.5 M EDTA
100 ¥ìl
7. Proteinase K (20 mg/ml)
200 ¥ìl
8. LINE-1 qPCR Primer (10 ¥ìM each)
*
50 ¥ìl
*
Real Time PCRÀ¸·Î »ó´ëÁ¤·® ºÐ¼®ÇÒ ¶§ °Ô³ð DNA¾çÀ» º¸Á¤Çϱâ À§ÇÑ reference·Î¼ »ç¿ëÇÑ´Ù.
Àΰ£ °Ô³ð DNA°ËÃâ¿ë primerÀ̹ǷΠ´Ù¸¥ »ý¹° Á¾¿¡¼´Â »ç¿ëÇÒ ¼ö ¾ø´Ù.
º¸Á¸
Component 1, 2 £º 4¡É
±×¿Ü ±¸¼ºÇ° £º £20¡É
°ü·ÃÁ¦Ç°
Thermal Cycler
¡¡
TaKaRa PCR Thermal Cycler Dice Gradient/Standard(Code TP600/TP650)
Àü±â¿µµ¿ ÀåÄ¡
¡¡
Mupid-One (Code AD160)
DNA Á¤Á¦ °ü·ÃÁ¦Ç°
¡¡ NucleoSpin Extract II(Code 740609.10/.50/.250, Macherey-Nagel Á¦Ç°)
Keyword :
Chromatin ¸é¿ª ħ°¨ (ChIP)
Chromatin Fragmentation (MNase)
Micrococcal Nuclease
ChIP GradeÀÇ Histone Ç×ü
Anti-Histone monoclonal antibody ½Ã¸®Áî
EpiXplore ChIP Assay Kit
Sonicator ¾øÀÌ È¿¼Ò·Î Genomic DNA¸¦ ´ÜÆíÈ ..
DNA Fragmentation Kit
Cross-link reversal & ChIP DNA elution
ChIP Elute Kit
Genome»óÀÇ Nucleosome À§Ä¡ ºÐ¼®
EpiScope¢ç Nucleosome Preparation Kit
ÃÑ 15ºÐÀ̸é Immunoprecipitation (IP, Co-IP) ..
Capturem¢â IP £¦ Co-IP Kit