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Home > ÀüÁ¦Ç°º¸±â > Epigenetics > miRNA ¹ßÇö ¡¤ Á¤·® > Small RNA-Seq Library Prep Kit
Small RNA (miRNA, siRNA, piRNA, sno RNA µî) ºÐ¼®À» À§ÇÑ NGS Library Á¦ÀÛ

Small RNA-Seq Library Prep Kit

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Clontech
635029
SMARTer¢ç smRNA-Seq Kit for Illumina¢ç
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12ȸ
(Package)
1,200,800¿ø 
1,501,000¿ø
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11.01 ~ 12.27
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Supplemental material for the SMARTer¢ç smRNA-seq Kit for Illumina¢ç (Click)

  • Analysis of diverse small RNA species: small RNA (15 - 150 nt) sequencing library Á¦ÀÛ
  • Flexible sample inputs: total RNA ¶Ç´Â enriched small RNA 1 ng - 2ug
  • Illumina¢ç-ready sequencing libraries: Illumina¢ç adaptor¿Í index¸¦ º°µµÀÇ Ligation °úÁ¤¾øÀÌ SMARTer¢ç ±â¼ú·Î ºÎÂø
  • Quick, single-tube workflow: 3½Ã°£ À̳»¿¡ sequencing library Á¦ÀÛ °¡´É
Á¦Ç°¼³¸í
Small non-coding RNAs (smRNAs) ¹ß°ß°ú ºÐ¼® ¿¬±¸´Â À¯ÀüÀÚ ¹ßÇöÀÇ Á¶Àý ±âÀÛÀ» ¿¬±¸ÇÏ´Â µ¥ ÀÖ¾î Å« ±â¿©¸¦ ÇØ¿Ô´Ù. ÁÖ·Î ¾Ë·ÁÁø small RNAs´Â miRNAs, siRNAs, piRNAs, snoRNAs·Î½á 20 - 30 ntÀÇ Å©±â¸¦ °¡Áö°í ÀÖ´Ù. SMARTer¢ç smRNA-Seq Kit for Illumina¢ç¸¦ ÀÌ¿ëÇÏ¸é ¼Ò·®ÀÇ small RNAs (low-input amounts)ºÎÅÍ °íÇ°ÁúÀÇ Illumina¢ç-ready sequencing library¸¦ Á¦ÀÛ ÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº 1ng - 2ugÀÇ total RNA ¶Ç´Â enriched small RNA sample·ÎºÎÅÍ SMART-Seq¢ç v4 ±â¼úÀ» ÀÌ¿ëÇÏ¿© Illumina¢ç sequencing library¸¦ Á¦ÀÛÇÑ´Ù. SMART technology (Switching Mechanism at 5¡¯ End of RNA Template)¿Í Locked Nucleic Acids (LNAs) ±â¼úÀÌ °áÇÕµÈ SMART-Seq¢ç v4 ±â¼úÀ» ÀÌ¿ëÇÔÀ¸·Î½á, ¼Ò·®ÀÇ small RNAs·ÎºÎÅÍ ÆíÇ⼺ÀÌ ¾ÆÁÖ ÀûÀº (minimal bias) NGS library¸¦ Á¦ÀÛ °¡´ÉÇÏ´Ù.


±×¸² 1. Schematic of technology used by the SMARTer¢ç smRNA-Seq Kit for Illumina¢ç. SMART technology is used in a ligation-free workflow to generate sequencing libraries for Illumina platforms


±×¸² 2. Demonstrating the accuracy of the SMARTer approach for small RNA-seq. Sequencing libraries were generated from an equimolar pool of 963 synthetic miRNAs (miRXplore Universal Reference) using the SMARTer¢ç smRNA-Seq Kit for Illumina¢ç (1 ng input; purple), or a small RNA-seq kit from a different vendor (Competitor N) employing an adapter ligation method (100 ng input; blue). Following sequencing, mapping, and counting of reads, miRNA expression levels (Y axis, log scale) were normalized, resulting in an expected expression level equal to 1 for each miRNA, and a 2-fold cutoff was assigned both above and below the expected expression level (indicated by two horizontal lines). For visualization purposes, miRNAs are ranked along the X axis in order of expression level.


±×¸² 3. Reproducibility of SMARTer¢ç small RNA-seq data. Sequencing libraries were generated in parallel from the indicated input amounts of human brain total RNA using the SMARTer smRNA-Seq Kit for Illumina, and size selected using the BluePippin system. Following sequencing, data processing, and mapping, expression levels of miRNAs identified for each library were quantified and plotted on correlation diagrams. Panel A. Correlation of miRNA expression levels for experimental replicates involving 1 ng inputs. Panel B. Correlation of miRNA expression levels for 2 ¥ìg vs. 1 ng inputs.
Application
Illumina-ready sequencing library preparation, specifically for small RNAs ranging in size from 15-150 nt
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
SMARTer¢ç smRNA-Seq Kit for Illumina ¢ç (Code 635029)
  • SMARTer smRNA-Seq Kit for Illumina Components
  • Indexing Primer Set HT for Illumina - 12
  • SeqAmp¢â DNA Polymerase
  • NucleoSpin Gel and PCR Clean-Up

Keyword :

miRNA ¹ßÇö ¡¤ Á¤·®
>
miRNA cDNA ÇÕ¼º°ú Á¤·®
MicroRNA qRT-PCR Á¤·®
>
Small RNA (miRNA, siRNA, piRNA, sno RNA µî) ..
Small RNA-Seq Library Prep Kit
>
ÆíÇ⼺ ³·°í Á¤È®¼ºÀÌ ³ôÀº, microRNA specific..
MicroRNA-Seq Library Prep Kit