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Single strand ChIP DNA¸¦ À§ÇÑ NGS Library Kit

DNA SMART ChIP-Seq Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
634865
DNA SMART ChIP-Seq Kit - 12
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
12ȸ
(Package)
1,231,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32618
Clontech
634866
DNA SMART ChIP-Seq Kit - 48 A
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
48ȸ
(Package)
4,210,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32618
Clontech
634867
DNA SMART ChIP-Seq Kit - 48 B
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
48ȸ
(Package)
4,210,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32618
Clontech
634887
ChIP Elute Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 Rxns
(Package)
840,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32576

ChIP Elute KitÀº chromatin immunoprecipitation (ChIP) ¹æ¹ýÀ¸·Î ¾ò¾îÁø DNA¸¦ ȸ¼ö, Á¤Á¦ÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°À» »ç¿ëÇϸé Single-stranded DNA (ssDNA)¸¦ ¾à 1½Ã°£¸¸¿¡ ȸ¼öÇÒ ¼ö ÀÖÀ¸¸ç, ȸ¼öµÈ DNA´Â DNA SMART ChIP-Seq Kit (code 634865)¿¡ ¹Ù·Î Àû¿ëÇÒ ¼ö ÀÖ´Ù.

DNA SMART ChIP-Seq KitÀº º°µµÀÇ ligation °úÁ¤ ¾øÀÌ(Ligation-independent manner), ¸ñÀû DNA ¾ç ¸»´Ü¿¡ Illumina-specific sequencing adaptor¸¦ ºÎ°¡ÇÏ¿© Illumina platform¿¡ ÀûÇÕÇÑ DNA Library¸¦ Á¦ÀÛÇÏ´Â Á¦Ç°ÀÌ´Ù. Chromatin Immunoprecipitation(ChIP)À¸·ÎºÎÅÍ È¸¼öÇÑ DNA´Â ¾çÀÌ ¸Å¿ì Àû±â ¶§¹®¿¡ Chromatin Immunoprecipitation-sequencing(ChIP-Seq)¿¡ Àû¿ëÇϱⰡ ¸Å¿ì ¾î·Æ´Ù. DNA SMART ChIP-Seq KitÀº ligation °úÁ¤ ¾øÀÌ, 100 pg - 10 ngÀÇ ¼Ò·®ÀÇ DNA »ùÇ÷κÎÅÍ Illumina platform¿ë sequencing library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ Àý´Ü ¶Ç´Â ºÐÇØµÈ double strand DNA(dsDNA) »Ó¸¸ ¾Æ´Ï¶ó single-strand DNA(ssDNA)µµ ÀÌ¿ëÇÒ ¼ö Àֱ⠶§¹®¿¡ ssDNA·ÎºÎÅÍ dsDNA·Î ¸¸µé±â À§ÇÑ º°µµÀÇ °úÁ¤ÀÌ ÇÊ¿ä ¾ø´Ù. º» Á¦Ç°À» ÀÌ¿ëÇÏ¸é ¾à 4½Ã°£¸¸¿¡ È¿À²ÀûÀ¸·Î library Á¦ÀÛÀÌ °¡´ÉÇÏ´Ù.

º» Á¦Ç°Àº Clontech ƯÇãÀÇ SMART ±â¼úÀ» Àû¿ëÇÑ »õ·Î¿î Á¦Ç°À¸·Î DNA ÁÖÇüÀ¸·ÎºÎÅÍ DNA¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù (DNA dependent DNA polymerase). DNA SMART ChIP-Seq KitÀÇ ÇÙ½ÉÀº ±Ø¼Ò·®ÀÇ »ùÇà (low-input sample)·ÎºÎÅÍ ligation °úÁ¤ ¾øÀÌ Illumina¿ë sequencing adaptor¸¦ ºÎ°¡ÇÒ ¼ö ÀÖ´Ù´Â Á¡ÀÌ´Ù.
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  • Ligation-independent adaptor addition for ChIP-seq library preparation
  • Flexible DNA inputs: 100 pg-10 ng of either ssDNA or dsDNA, or total DNA from 10,000-1,000,000 cell ChIP experiments
  • Simple Protocol: Generate Illumina-ready sequencing libraries in around 4 hours

±×¸² 1. This single-tube workflow. allows users to generate Illumina-compatible libraries for ChIP-seq experiments. After library size selection and purification, the total time from input DNA to ChIP-seq library is approximately four hours.


Ç¥ 1. The DNA SMART ChIP-Seq Kit uses a combined size selection and clean-up step after library amplification by PCR. Compared to other protocols that perform size selection before PCR amplification, post-PCR size selection results in a higher yield while maintaining the quality of the libraries.


Ç¥ 2. The DNA SMART ChIP-Seq Kit has the sensitivity to generate sequencing libraries from very small amounts of fragmented DNA. The number of unique, non-duplicate reads is high across all input levels, and the number of peaks identified is similar across input amounts.
Àû¿ë
  • Sequencing library preparation for ChIP-seq from low-input, fragmented DNA
  • Illumina-specific NGS sequencing library production
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ±¸¼ºÇ°Àº CoA ¶Ç´Â ¸Å´º¾ó ÂüÁ¶)
- DNA SMART ChIP-Seq Components
- Indexing Primer Set HT for Illumina
- SeqAmp DNA Polymerase

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Note

634865

DNA SMART ChIP-Seq Kit - 12

the reagents to generate up to 12 indexed libraries using a single forward primer and the 12 reverse indexing primers

634866

DNA SMART ChIP-Seq Kit - 48 A

generate up to 48 indexed libraries using the first 4 forward primers and the 12 reverse indexing primers

634867

DNA SMART ChIP-Seq Kit - 48 B

generate up to 48 indexed libraries using the second 4 forward primers and the 12 reverse indexing primers


Keyword :

ChIP-seq, Cut&Run-seq (NGS)
>
50pg ChIP DNA-seq ºÐ¼®À» À§ÇÑ NGS Library Ki..
SMARTer¢ç ThruPLEX¢ç DNA-seq Kit
>
Single strand ChIP DNA¸¦ À§ÇÑ NGS Library Ki..
DNA SMART ChIP-Seq Kit
>
Illumina¢ç NGS library Á¦ÀÛ¿ë Indexing prime..
SMARTer¢ç DNA index kits