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Home > ÀüÁ¦Ç°º¸±â > Epigenetics > DNA Methlyation ¿¬±¸ > TaKaRa EpiTaq HS (for bisulfite-treated DNA)
Bisulfite ó¸® ÈÄÀÇ DNA PCR ÁõÆø¿¡ ÃÖÀû

TaKaRa EpiTaq HS (for bisulfite-treated DNA)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R110A
TaKaRa EpiTaq HS (for bisulfite-treated DNA)
°ü·ÃÇмú ±¸¸ÅÇϱâ
250 U
270,400¿ø 
338,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
R110A_e.v1910Da.pdf
Takara
R110B
TaKaRa EpiTaq HS (for bisulfite-treated DNA)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U (R110A x 4)
1,216,000¿ø 



  • Uracil(U)À» ¸¹ÀÌ Æ÷ÇÔÇÑ DNA ÁõÆø¿¡ ÃÖÀû
  • Anti-Taq antibody¸¦ ÀÌ¿ëÇÑ hot start PCR È¿¼Ò·Î ºñƯÀÌÀû ÁõÆø ¾ïÁ¦
  • GC-rich target, AT-rich targetÀÇ È¿À²ÀÎ ÁõÆø °¡´É
  • ÁõÆø »ê¹°ÀÇ 3£§¸»´Ü¿¡ A°¡ ºÎ°¡, ±×´ë·Î TA Ŭ·Î´×¿¡ »ç¿ë °¡´É
Á¦Ç°¼³¸í
TaKaRa EpiTaq¢â HS (for bisulfite-treated DNA)´Â bisulfite sequencingÀ̳ª COBRA¹ý µîÀÇ ºÐ¼®À» À§ÇØ bisulfite ó¸® ÈÄ UracilÀ» ¸¹ÀÌ Æ÷ÇÔÇÏ´Â DNA¸¦ ÁÖÇüÀ¸·Î PCR ÁõÆø½Ã ÃÖÀûÀÎ PCR È¿¼ÒÀÌ´Ù.
Bisulfite ó¸®ÇÑ DNA´Â PCR ¹ÝÀÀ¼º¿¡ º¯È­°¡ ÀÖÀ» ¼ö ÀÖÁö¸¸, º» È¿¼Ò´Â ¸¶±×³×½· ³óµµ³ª dNTP ³óµµ¸¦ Á¶Á¤ÇÔÀ¸·Î¼­ ÁõÆø È¿À², ¹ÝÀÀ ƯÀ̼ºÀ» Á¶ÀýÇÒ ¼ö Àֱ⠶§¹®¿¡ Áö±Ý±îÁö ÁõÆøÇÒ ¼ö ¾ø¾ú´ø Ÿ°Ù¿¡¼­µµ ¾çÈ£ÇÑ ÁõÆøÀ» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù.
º» È¿¼Ò´Â anti-Taq antibody¸¦ Æ÷ÇÔÇÑ hot-start PCR È¿¼Ò·Î °í¿Â °¡¿­ÇÒ ¶§±îÁö´Â anti-Taq antibody°¡ È¿¼Ò¿¡ °áÇÕÇØ polymerase È°¼ºÀ» ¾ïÁ¦Çϱ⠶§¹®¿¡, PCR cycle ÀüÀÇ mis-primmingÀ̳ª primer dimer¿¡¼­ À¯·¡ÇÏ´Â ºñƯÀÌÀû ÁõÆøÀ» ¸·À» ¼ö ÀÖ´Ù. Anti-Taq antibody´Â PCR initial denaturation °úÁ¤¿¡¼­ º¯¼ºµÇ¾î ºÐ¸®µÇ¹Ç·Î, È¿¼Ò È°¼ºÈ­¸¦ À§ÇÑ º°µµÀÇ °úÁ¤À» Ãß°¡ÇÒ ÇÊ¿ä°¡ ¾ø´Ù.

[ÁÖÀÇ] Bisulfite ó¸®ÇÑ DNA¸¦ ÀÌ¿ëÇÏ¿©, ¸ÞÆ¿È­ ƯÀÌÀû PCR (Methylation Specific PCR: MSP) ºÐ¼®À» ÁøÇàÇÒ °æ¿ì¿¡´Â EpiScope¢ç MSP Kit (Code R100A/B)ÀÇ »ç¿ëÀ» ÃßõÇÑ´Ù.
³»¿ë (50 §¡ ¹ÝÀÀ °è, 200ȸ)

1. TaKaRa EpiTaq HS (5 U/§¡)

250 U

2. 10¡¿EpiTaq PCR Buffer (Mg2+ free)

1 §¢

3. dNTP Mixture (°¢ 2.5 mM)

1.2 §¢

4. 25 mM MgCl2

1.2 §¢

º¸Á¸ - 20¡É
½ÇÇè ¿¹
1. Bisulfite ó¸®ÇÑ HeLa genomic DNAÀÇ PCR
TaKaRa EpiTaq¢â HS (for bisulfite-treated DNA)´Â 1 kb°¡ ³Ñ´Â Ÿ°ÙÀ» Æ÷ÇÔ, ´Ù¾çÇÑ »çÀÌÁîÀÇ Å¸°Ù ¿µ¿ª¿¡¼­ Àß ÁõÆøµÇ´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù.


ÁÖÇü

: Bisulfite ó¸®ÇÑ HeLa genomic DNA (100 ng/50 §¡ ¹ÝÀÀ¾×)

Ÿ°Ù

: CDH1, MLH1, BRCA1 gene »ó·ùÀÇ CpG island ¿µ¿ª


2. Bisulfite ó¸® DNAÀÇ È¿À²ÀûÀÎ ÁõÆø
TaKaRa EpiTaq¢â HS¿Í Ÿ»ç bisulfite ó¸® DNA¿ë PCR È¿¼Ò·Î bisulfite ó¸®ÇÑ HeLa genomic DNA¸¦ ÁõÆøÇÑ °á°ú, µÎ°³ÀÇ Å¸°Ù ¸ðµÎ TaKaRa EpiTaq¢â HS¿¡¼­ °¡Àå ¾çÈ£ÇÑ ÁõÆøÀ» È®ÀÎÇÏ¿´´Ù.


°ü·ÃÁ¦Ç°
- ¹ü¿ëÀûÀÎ ¿Ã¶ó¿î´õ agarose: SeaKem LE Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â: Clontech PCR Thermal Cycler GP

Keyword : epigenetics,ÈļºÀ¯ÀüÇÐ,methylation,bisulfite DNA,methylated DNA

DNA Methlyation ¿¬±¸
>
Bisulfite ó¸® ÈÄÀÇ DNA PCR ÁõÆø¿¡ ÃÖÀû
TaKaRa EpiTaq HS (for bisulfite-treated DNA)
>
ƯÁ¤ ¿µ¿ªÀÇ DNA MethylationÀ» Çؼ® °¡´É
EpiScope¢ç MSP Kit
>
genomic DNA ¶Ç´Â CpG island ¿µ¿ªÀÇ ÁõÆø¿¡ ÃÖ..
TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time)
>
DNA ¸ÞÆ¿È­ Çؼ® ÄÁÆ®·Ñ
¸ÞÆ¿È­/ºñ¸ÞÆ¿È­ ÄÁÆ®·Ñ °Ô³ð DNA