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Real Time PCR
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Cell direct qPCR
> Whole Transcriptome ÁõÆø½Ã¾à
1 °³ÀÇ ¼¼Æ÷·ÎºÎÅÍ template cDNA¸¦ ÁõÆø °¡´É
Whole Transcriptome ÁõÆø½Ã¾à
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Takara
3734
CellAmp¢â Whole Transcriptome Amplification Kit (Real Time) Ver.2
100 ȸ
(5 §¡ ¹ÝÀÀ°è)
625,600¿ø
782,000¿ø
09.02 ~ 10.31
Product index
Overview
FASTest Real Time RT-P..
Dye-based Real Time PC..
Real Time PCR¿ë cDNA ..
One-step Real Time PCR..
Probe °ËÃâ¿ë Real Time..
Real Time PCR accessor..
The Best Tool for qPCR
qPCR Preamplification
PreAmp Master Mix
Real Time PCR ±â±â
Thermal Cycler Dice¢ç ..
Thermal Cycler Dice¢ç ..
CronoSTAR¢â 96 Real-Ti..
SmartChip¢â Real Time ..
cDNA ÇÕ¼º (Real Time PCR ..
gDNA Á¦°Å& RT ½Ã¾à (qP..
5x RT Master Mix (qPCR..
RT components (qPCR¿ë)
[New] ½Ã°£´ÜÃà, gDNA ..
»ùÇà dilution ½Ã¾à (qP..
EASY Dilution II (for ..
Intercalator TB Green °ËÃâ
Standard qPCR Mix
Long target¿ë qPCR Mix
Fast qPCR Mix
Dimer eraser qPCR Mix
GC rich qPCR Mix
Crude »ùÇÿë qPCR Mix
Premixed 1 step qRT-PC..
Specific 1 step qRT-PC..
Standard 1 step qRT-PC..
External Standard Kit ..
Transgene Detection Pr..
[New] Fast, UNG, dye p..
Dual-Labeled Probe °ËÃâ
Probe qPCR Mix MultiPl..
Probe qPCR Mix (¶Ç´Â U..
2 x all premix 1 step ..
Premix Ex Taq¢â (Probe..
[New] ½Ã°£´ÜÃà, gDNA ..
One Step PrimeScript¢â..
gDNA Á¦°Å& RT ½Ã¾à (qP..
5x RT Master Mix (qPCR..
RT components (qPCR¿ë)
PrimeDirect¢ç Probe RT..
Cell direct qPCR
RNA ÃßÃâ¾øÀÌ cell dire..
qPCR/Western¿ë cell ly..
1 step qRT-PCR¿ë cell ..
qPCR¿ë cell lysate Á¶..
Whole Transcriptome Áõ..
TB Green °ËÃâ(Clontech)
TB Green¢ç Advantage q..
Terra qPCR Direct TB G..
CycleavePCR °ËÃâ
Cycling Probe ¹ý¿¡ ´ë..
CycleavePCR Core Kit
CycleavePCR GBS Capsul..
CycleavePCR Streptococ..
CycleavePCR¢â Reaction..
CycleavePCR¢â Salmonel..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (Real ..
Bacteria (
tuf
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Provirus Copy Number D..
Transgene Detection Pr..
1~1,000°³ Á¤µµ ¼¼Æ÷ ȤÀº 20 pg~20 ngÀÇ total RNA¿¡¼ È¿À²ÀûÀ¸·Î cDNA ÁõÆø °¡´É
¹Ì·® »ùÇÿ¡¼µµ ´Ù¾çÇÑ À¯ÀüÀÚÀÇ Real Time PCR Çؼ® °¡´É
ÇÁ·ÎÅäÄÝ ÃÖÀûÈ ÅëÇØ Àú¹ßÇö À¯ÀüÀÚ±îÁö È¿À²ÀûÀ¸·Î ÁõÆø °¡´É
Á¦Ç° ¼³¸í
º» Á¦Ç°Àº ¼Ò¼öÀÇ ¼¼Æ÷(1~1000°³)¸¦ ÀÌ¿ëÇÏ¿© ¹Ù·Î cDNA¸¦ ÁõÆøÇÏ´Â Á¦Ç°ÀÌ´Ù. ÁõÆøµÈ cDNA´Â Real Time PCR ÁÖÇüÀ¸·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù. ÀϹÝÀûÀ¸·Î ¹Ì·®ÀÇ ÇÙ»êÀ» ÀÌ¿ëÇÒ ¶§, Á¤Á¦¿¡ ÀÇÇÑ ¼Ò½ÇÀÌ ¹®Á¦°¡ µÇÁö¸¸, ÀÌ Á¦Ç°À» ÀÌ¿ëÇϸé RNA³ª cDNA Á¤Á¦¾øÀÌ È¿À²ÀûÀ¸·Î cDNA¸¦ ÁõÆøÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°¿¡¼´Â ¿ì¼± ¼¼Æ÷¸¦ ¿ëÇØÇÏ¿©, dT-adaptor primer(RT dT Primer 2)¸¦ ÀÌ¿ëÇÑ ¿ªÀü»ç ¹ÝÀÀ ÈÄ, mRNA·ÎºÎÅÍ cDNA¸¦ ÇÕ¼ºÇÑ´Ù. ±× ´ÙÀ½¿¡ ÇÕ¼ºÇÑ cDNA¿¡ TdT·Î dA-tail¸¦ ºÎ°¡ÇÑ ÈÄ, ÀÌ°ÍÀ» ÁÖÇüÀ¸·Î ÇÏ¿© PCR cycle ¼ö¸¦ ÁÙ¿© cDNA¸¦ ÁõÆøÇÑ´Ù.
º» Á¦Ç°Àº ¼¼Æ÷¿¡¼ Á÷Á¢ cDNA ÁõÆøÇÒ ¼öµµ ÀÖ°í, ¹Ì·®ÀÇ RNA(20 pg~20 ng)¿¡¼ cDNA ÁõÆøÇÒ ¼öµµ ÀÖ´Ù.
Ver.2¿¡¼´Â Exonuclease Ió¸® °úÁ¤À» Ãß°¡ÇÏ¿©, ±âÁ¸ Á¦Ç°¿¡ ºñÇØ ÇÁ¶óÀÌ¸Ó À¯·¡ÀÇ ºñƯÀÌÀû ÁõÆøÀ» ¾ïÁ¦ÇÏ¿© ¹ßÇö·®ÀÇ ÀûÀº À¯ÀüÀÚÀÇ ÁõÆø È¿À²À» Çâ»ó½ÃÄ×´Ù.
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(5 ¥ìl¹ÝÀÀ½Ã, 100ȸºÐ)
1. Lysis Buffer£¨4¡¿£©
150 ¥ìl
2. Recombinant RNase Inhibitor£¨40 U/¥ìl£©
30 ¥ìl
3. RT dT Primer 2
12 ¥ìl
4. dNTP Mixture£¨2.5 mM each£©
12 ¥ìl
5. MgCl
2
£¨22.5 mM£©
36 ¥ìl
6. RT Enzyme Mix 2
*1
36 ¥ìl
7. Exonuclease I£¨5 U/¥ìl£©
72 ¥ìl
8. TdT Buffer£¨5¡¿£©
144 ¥ìl
9. dATP£¨90 mM£©
24 ¥ìl
10. TdT Enzyme Mix
*2
54 ¥ìl
11. PCR Primer Mix 2
300 ¥ìl
12. RNase Free dH
2
O
1 ml
*1
PrimeScript Reverse Transcriptase¡¢RNase Inhibitor Æ÷ÇÔ.
*2
Terminal Deoxynucleotidyl Transferase¡¢RNase H Æ÷ÇÔ.
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- 20 ¡É
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PCR ½Ã¾à
- TaKaRa Ex Taq Hot Start Version(Code RR006A/B)
1.5 ml microtube
0.2 ml microtube
Micropipette ¹× tip
PCR Thermal Cycler
- TaKaRa PCR Thermal Cycler Dice Gradient/Standard(Á¦Ç° ÄÚµå TP600/TP650) µî
½ÇÇè°úÁ¤
Real Time RT-PCR¿ë primer Á¦ÀÛ¼ºñ½º¡¸Perfect Real Time Support System¡¹
Perfect Real Time Support SystemÀº intercalator ¹ý(SYBR Green I°ËÃâ)À» ÀÌ¿ëÇÑ Real Time RT-PCR¿ëÀ¸·Î ÃÖÀûÈµÈ ÇÁ¶óÀ̸Ӹ¦
°Ë»ö¡¤¿Â¶óÀÎ ÁÖ¹®Çϱâ À§ÇÑ ½Ã½ºÅÛÀÌ´Ù.
Human, mouse, rat, dog, chicken, bovineÀÇ RefSeq µî·Ï À¯ÀüÀÚ¿¡ ´ëÇÑ Àü¹ÝÀûÀÎ Real Time RT-PCR¿ë ÇÁ¶óÀÌ¸Ó ¼³°è°¡ °¡´ÉÇÏ´Ù.
Ÿ°Ù À¯ÀüÀÚ¸¦ °Ë»öÇÏ¿©, ¿øÇÏ´Â ÇÁ¶óÀÌ¸Ó ¼¼Æ®¸¦ ¼±ÅÃÇÏ¸é °£´ÜÇÏ°Ô ¿Â¶óÀÎÀ¸·Î ÁÖ¹® °¡´ÉÇÏ´Ù. ¹ø°Å·Î¿î ÇÁ¶óÀÌ¸Ó ¼³°è¿Í ¹ÝÀÀ Á¶°Ç ÃÖÀûÈ°¡ ºÒÇÊ¿ä(TAKARA Real Time PCR reagent¿¡ ÃÖÀûÈ) Çϸç, siRNAÀÇ È¿°úÀÇ È®ÀÎ, DNA Microarray Çؼ® °á°ú °ËÁõ µî À¯ÀüÀÚ ¹ßÇö Á¤·®À̳ª À¯ÀüÀÚ °ËÃâ¿¡ °£ÆíÇÏ°Ô »ç¿ëÇÒ ¼ö ÀÖ´Ù.
[Real Time Support System ¹Ù·Î°¡±â]
Keyword :
Cell direct qPCR
1.5½Ã°£ÀÌ¸é ¹è¾ç¼¼Æ÷¿¡¼ Real Time PCR °á°ú ..
RNA ÃßÃâ¾øÀÌ cell direct qPCR
°£´ÜÇÏ°Ô Á¶Á¦ÇÑ Lysate¿¡¼ mRNA¿Í ´Ü¹éÁúÀÇ ..
qPCR/Western¿ë cell lysate Á¶Á¦½Ã¾à
¹è¾ç¼¼Æ÷·ÎºÎÅÍ °£´ÜÇÏ°Ô 1 Step Real Time RT-..
1 step qRT-PCR¿ë cell lysate Á¶Á¦½Ã¾à
ºÒ°ú 10 ºÐ ¾È¿¡ ¹è¾ç ¼¼Æ÷·ÎºÎÅÍ Real Time RT..
qPCR¿ë cell lysate Á¶Á¦½Ã¾à
1 °³ÀÇ ¼¼Æ÷·ÎºÎÅÍ template cDNA¸¦ ÁõÆø °¡´É
Whole Transcriptome ÁõÆø½Ã¾à