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X-α-Gal

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Clontech
630463
X-α-Gal
관련학술 구매하기
250 mg
528,000원  제조사 페이지로 바로가기 x33190

X-α-Gal is a chromogenic substrate for yeast galactosidase (MEL1) and is used for detecting GAL4- based two-hybrid interactions directly on agar (Figure 1). This quick and easy assay eliminates the need for time-consuming β-galactosidase liquid and filter-lift assays in two-hybrid analysis.

The X-α-Gal assay detects the activation of the yeast MEL1 gene, a GAL4-regulated gene used in two-hybrid analysis. MEL1 encodes the secreted enzyme α-galactosidase, which hydrolyzes colorless X-α-Gal into a blue end-product. Yeast colonies that express α-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-α-Gal (Table 1).


Figure 1. X-α-Gal detects secreted α-galactosidase activity following a GAL4-based two-hybrid interactions in Y2HGold yeast patches and colonies.
Storage Conditions
-20°C
References
1. Aho, S., et al. (1997) Anal. Biochem. 253:270-272.
2. Liljestrom, P. L. (1985) Nucleic Acids Res. 13:7257-7268.

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