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Inducible System ¡¤ Yeast hybrid
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Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
> IRES Tet À¯µµ¹ßÇö ½Ã½ºÅÛ
IRES Tet À¯µµ¹ßÇö ½Ã½ºÅÛ
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Clontech
631112
Tet-On Advanced IRES Fluorescent Vector Set
°¢ 40 ¥ìl
1,165,000¿ø
,
,
,
Clontech
631113
Tet-Off Advanced IRES Fluorescent Vector Set
°¢ 40 ¥ìl
1,165,000¿ø
,
,
,
Product index
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Syste..
Tet-On 3G À¯µµ ¹ßÇö ½Ã..
Lenti-X Tet-On 3G Indu..
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Line..
Tet-One Inducible Expr..
TetR Ç×ü
[Legacy] Tetracycline ..
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½º..
iDimerize Inducible System..
[°³¿ä] Inducible Prote..
iDimerize Antibodies
Inducible Expression S..
Heterodimer System
Homodimer System
Regulated Transcriptio..
Reverse Dimerization S..
´Ü¹éÁú ¹ßÇö·® Á¶Àý ½Ã½ºÅÛ ..
ProteoTuner System
Tet-ProteoTuner Vector..
[°³¿ä] Yeast Hybrid System
[FAQ] Matchmaker Gold ..
[°³¿ä] Matchmaker Gold..
Yeast Two Hybrid System (Y..
Y2H Library Constructi..
Yeast Culture media (T..
Yeast Two-Hybrid (Y2H)..
Y2H Library (Yeast ³»..
Normalized Y2H Library..
Yeast Two hybrid vecto..
pBridge Vector
Yeast One-Hybrid (Y1H)
Yeast Culture Media (O..
Yeast One-Hybrid (Y1H)..
Matchmaker °ü·ÃÁ¦Ç°
Yeast Culture Media
Yeast Culture Media (..
Aureobasidin A
Yeast Plasmid Isolati..
Matchmaker Ç×ü
Insert Check Kit (prey..
Yeast Transformation M..
X-¥á-Gal
Yeast Transformation S..
Mammalian Cell Assay K..
Çü±¤ marker¸¦ ÀÌ¿ëÇÏ¿© Tet-inducible cloneÀ» ¼±º°ÇÏ´Â ½Ã°£ ´ÜÃà
Tet-On°ú Tet-Off Advanced ±â¼úÀ» ÀÌ¿ëÇÑ ¶Ù¾î³ ¹ßÇö Á¶Àý
Transactivator ¹ßÇö°ú À¯ÀüÀÚÀÇ À¯µµ¹ßÇöÀ» µ¿½Ã¿¡ ÃßÀû °¡´É
Tet-Dual Expression System (Tet-Advanced IRES Fluorescent Vector Set)Àº Tet-On Advanced¿Í Tet-Off Advanced Á¦Ç° Áß °¡Àå Çõ½ÅÀûÀÎ Á¦Ç°À¸·Î ¸Å¿ì °·ÂÇÏ°Ô À¯ÀüÀÚÀÇ ¹ßÇöÀ» Á¶ÀýÇÒ ¼ö ÀÖÀ»»Ó¸¸ ¾Æ´Ï¶ó, ¹àÀº Àû»ö(mCherry)°ú ³ì»ö (ZsGreen1) Çü±¤ ´Ü¹éÁúÀÌ ÇÔ²² ¹ßÇö µÇ¹Ç·Î ½±°Ô cloneÀ» ¼±º°ÇÒ ¼ö ÀÖ¾î inducible system ±¸Ãà¿¡ ÇÊ¿äÇÑ ½Ã°£À» ´ëÆø °¨Ãà½ÃŲ´Ù.
µÎ °³ÀÇ ´Ü¹éÁúÀÌ µ¿½Ã¿¡ ¹ßÇöµÇ´Â ¿ø¸®
Tet-Dual SystemÀÇ ÇÙ½É ¿ä¼Ò´Â ÇϳªÀÇ mRNA transcript·ÎºÎÅÍ ¸ñÀûÀ¯ÀüÀÚ¿Í Çü±¤´Ü¹éÁúÀÌ ÇÔ²² ¹ßÇöµÇµµ·Ï ÇÏ´Â internal ribosome entry site(IRES) ÀÌ´Ù. ÁøÇÙ»ý¹°ÀÇ mRNA ¹ø¿ªÀº °ÅÀÇ 5£§-cap¿¡¼¸¸ ½ÃÀÛµÇÁö¸¸, IRES´Â ³»ºÎ ´Ù¸¥ site¿¡¼ ribosomeÀÌ °áÇÕÇÏ¿© ¹ø¿ªÀ» ½ÃÀÛÇÒ ¼ö ÀÖµµ·Ï ÇÑ´Ù. °á°úÀûÀ¸·Î µÎ °³ÀÇ ´Ü¹éÁúÀÌ ÇϳªÀÇ bicistronic mRNA transcript·ÎºÎÅÍ µ¿½Ã¿¡ ¹ßÇöµÇ°Ô µÈ´Ù (Figure 1 & 2)
Ç×»ó ³ôÀº À¯µµÀ²
¾ö°ÝÇÑ Á¶Àý ¹× ³ôÀº ¹ßÇö ¼öÁØÀº activatior¿Í inducible expression vector°¡ ÇÔ²² transfectionµÇ¾î Çü¼ºµÈ ¼¼Æ÷ÀÇ ¼ö¿Í stable cell lineÀÇ ±¸Ãà ¿©ºÎ¿¡ ´Þ·Á ÀÖ´Ù. µû¶ó¼ ´õ ¾ö°ÝÇÑ Á¶Àý°ú °í¹ßÇöÀÌ ÇÊ¿äÇÑ °æ¿ì, °¢°¢ÀÇ vector¸¦ µ¶¸³ÀûÀ¸·Î transfection ÇÏ°í, ÃÖÀû Á¶°ÇÀÇ cloneÀ» ¼±º°ÇÏ´Â °úÁ¤µµ º°µµ·Î ¼öÇàÇÏ´Â °ÍÀÌ ÁÁ´Ù.
Figure 1. Induction with Tet-Dual simultaneously produces two proteins from a bicistronic mRNA.
Figure 2. Tet-Dual System vectors.
The Tet-Dual transactivator expression vectors constitutively express either Tet-On Advanced or Tet-Off Advanced, and the fluorescent protein, ZsGreen1 (Panel A). pTRE-Dual2 is a Tet-regulated inducible expression vector that coexpresses mCherry and a second gene that is cloned in the multiple cloning site (MCS) (Panel B). pTRE-Dual1 coexpresses two user-defined genes.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Keyword :
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Systems Product
Tet-On 3G À¯µµ ¹ßÇö ½Ã½ºÅÛ
Lenti-X Tet-On 3G Inducible Expression System
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Linear / Plasmid)
All-in-one vector ÇüÅÂÀÇ Tet-inducible syste..
Tet-One Inducible Expression (One vector System)
TetR Ç×ü
[Legacy] Tetracycline À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½ºÅÛ