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IRES Tet À¯µµ¹ßÇö ½Ã½ºÅÛ

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
631112
Tet-On Advanced IRES Fluorescent Vector Set
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
°¢ 40 ¥ìl
1,165,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33573, x33604, x33605, x33152
Clontech
631113
Tet-Off Advanced IRES Fluorescent Vector Set
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
°¢ 40 ¥ìl
1,165,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33572, x33604, x33605, x33149

  • Çü±¤ marker¸¦ ÀÌ¿ëÇÏ¿© Tet-inducible cloneÀ» ¼±º°ÇÏ´Â ½Ã°£ ´ÜÃà
  • Tet-On°ú Tet-Off Advanced ±â¼úÀ» ÀÌ¿ëÇÑ ¶Ù¾î³­ ¹ßÇö Á¶Àý
  • Transactivator ¹ßÇö°ú À¯ÀüÀÚÀÇ À¯µµ¹ßÇöÀ» µ¿½Ã¿¡ ÃßÀû °¡´É
Tet-Dual Expression System (Tet-Advanced IRES Fluorescent Vector Set)Àº Tet-On Advanced¿Í Tet-Off Advanced Á¦Ç° Áß °¡Àå Çõ½ÅÀûÀÎ Á¦Ç°À¸·Î ¸Å¿ì °­·ÂÇÏ°Ô À¯ÀüÀÚÀÇ ¹ßÇöÀ» Á¶ÀýÇÒ ¼ö ÀÖÀ»»Ó¸¸ ¾Æ´Ï¶ó, ¹àÀº Àû»ö(mCherry)°ú ³ì»ö (ZsGreen1) Çü±¤ ´Ü¹éÁúÀÌ ÇÔ²² ¹ßÇö µÇ¹Ç·Î ½±°Ô cloneÀ» ¼±º°ÇÒ ¼ö ÀÖ¾î inducible system ±¸Ãà¿¡ ÇÊ¿äÇÑ ½Ã°£À» ´ëÆø °¨Ãà½ÃŲ´Ù.
µÎ °³ÀÇ ´Ü¹éÁúÀÌ µ¿½Ã¿¡ ¹ßÇöµÇ´Â ¿ø¸®
Tet-Dual SystemÀÇ ÇÙ½É ¿ä¼Ò´Â ÇϳªÀÇ mRNA transcript·ÎºÎÅÍ ¸ñÀûÀ¯ÀüÀÚ¿Í Çü±¤´Ü¹éÁúÀÌ ÇÔ²² ¹ßÇöµÇµµ·Ï ÇÏ´Â internal ribosome entry site(IRES) ÀÌ´Ù. ÁøÇÙ»ý¹°ÀÇ mRNA ¹ø¿ªÀº °ÅÀÇ 5£§-cap¿¡¼­¸¸ ½ÃÀÛµÇÁö¸¸, IRES´Â ³»ºÎ ´Ù¸¥ site¿¡¼­ ribosomeÀÌ °áÇÕÇÏ¿© ¹ø¿ªÀ» ½ÃÀÛÇÒ ¼ö ÀÖµµ·Ï ÇÑ´Ù. °á°úÀûÀ¸·Î µÎ °³ÀÇ ´Ü¹éÁúÀÌ ÇϳªÀÇ bicistronic mRNA transcript·ÎºÎÅÍ µ¿½Ã¿¡ ¹ßÇöµÇ°Ô µÈ´Ù (Figure 1 & 2)
Ç×»ó ³ôÀº À¯µµÀ²
¾ö°ÝÇÑ Á¶Àý ¹× ³ôÀº ¹ßÇö ¼öÁØÀº activatior¿Í inducible expression vector°¡ ÇÔ²² transfectionµÇ¾î Çü¼ºµÈ ¼¼Æ÷ÀÇ ¼ö¿Í stable cell lineÀÇ ±¸Ãà ¿©ºÎ¿¡ ´Þ·Á ÀÖ´Ù. µû¶ó¼­ ´õ ¾ö°ÝÇÑ Á¶Àý°ú °í¹ßÇöÀÌ ÇÊ¿äÇÑ °æ¿ì, °¢°¢ÀÇ vector¸¦ µ¶¸³ÀûÀ¸·Î transfection ÇÏ°í, ÃÖÀû Á¶°ÇÀÇ cloneÀ» ¼±º°ÇÏ´Â °úÁ¤µµ º°µµ·Î ¼öÇàÇÏ´Â °ÍÀÌ ÁÁ´Ù.


Figure 1. Induction with Tet-Dual simultaneously produces two proteins from a bicistronic mRNA.


Figure 2. Tet-Dual System vectors. The Tet-Dual transactivator expression vectors constitutively express either Tet-On Advanced or Tet-Off Advanced, and the fluorescent protein, ZsGreen1 (Panel A). pTRE-Dual2 is a Tet-regulated inducible expression vector that coexpresses mCherry and a second gene that is cloned in the multiple cloning site (MCS) (Panel B). pTRE-Dual1 coexpresses two user-defined genes.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.

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