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Ç×ü ¡¤ ELISA ¡¤ Signal
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Apoptosis
> ApopLadder Ex¢â
Apoptosis·Î ÀÎÇÑ ´ÜÆíÈ DNA °ËÃâ
ApopLadder Ex¢â
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Takara
MK600
ApopLadder Ex¢â
24 ȸ
272,800¿ø
341,000¿ø
09.02 ~ 10.31
Product index
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TaKaRa DAB Substrate
TaKaRa POD Conjugate½Ã..
½Å°æ¹ß»ýºÐÈ ¸¶Ä¿ °ü·Ã Ç×..
½Å°æ¹ß»ýºÐÈ ¸¶Ä¿ °ü·Ã..
EIA Kit
Acrolein-Lysine Adduct..
Fibronectin EIA Kit
Human Albumin EIA Kit
Human IgG EIA Kit
Human Vitronectin EIA ..
Human/Pig Osteonectin ..
Laminin(LN) EIA Kit
Mouse Albumin EIA Kit
Mouse Gla-Osteocalcin ..
Osteocalcin EIA Kit
Peptide Coating Kit
Pig Osteocalcin EIA Ki..
Procollagen Type I C-p..
Rat Gla-Osteocalcin Hi..
Rat Heme Oxygenase-1 E..
RetroNectin¢ç Á¤·® kit
Universal Tyrosine Kin..
Wash and Stop Solution..
Osteo¤ýAdipo assay
AdipoRed Assay Reagent
Osteoblast-Inducer Rea..
OsteoImage Mineralizat..
PDELight HTS cAMP Phos..
PPiLight Inorganic Pyr..
TRACP & ALP Assay Kit
TRACP & ALP double-sta..
¼¼Æ÷Áõ½Ä ¡¤ ¼¼Æ÷ÀåÇØ ÃøÁ¤
Premix WST-1 Cell Prol..
Luminescent Assay Acce..
Premix WST-1 Cell Prol..
ToxiLight BioAssay Kit
ViaLight ½Ã¸®Áî
È¿¼ÒÈ°¼º ÃøÁ¤ ¡¤ °ËÃâ¿ë Ki..
Heparan Degrading Enzy..
Apoptosis
ApoAlert Annexin V-FIT..
ApoAlert Caspase Assay..
ApoAlert pDsRed2-Bid V..
ApopLadder Ex¢â
ApoPrimer Set (Bcl-2 f..
In situ Apoptosis Dete..
Signal Transduction
Dominant-Negative Vect..
ExpressHyb Hybridizati..
Signal Àü´Þ °æ·Î Profi..
Ç×ü
Ç×ü ±³Â÷ ¹ÝÀÀ À϶÷Ç¥
Ç×ü ¿ëµµ À϶÷Ç¥
Anti-Albumin
Anti-Bovine Osteocalci..
Anti-CD3 mAb GMP grade
Anti-Chicken N-cadheri..
Anti-Heme Oxygenase-1
Anti-Human E-cadherin
Anti-Human Fibronectin
Anti-Human Influenza V..
Anti-Human Laminin
Anti-Human N-cadherin
Anti-Human Osteocalcin
Anti-Human P-cadherin
Anti-Human Platelet GM..
Anti-Human Procollagen..
Anti-Human Vitronectin
Anti-Human von Willebr..
Anti-Insulin C
Anti-Mouse E-cadherin
Anti-Mouse Osteocalcin
Anti-Mouse P-cadherin
Anti-Osteonectin/SPARC
Anti-ProS2
Anti-Rat Collagen type..
Anti-Rat Osteocalcin
Monoclonal 2nd antibod..
Polyclonal Anti-Dentin..
Polyclonal Anti-Glucag..
Polyclonal Anti-Human ..
Polyclonal Anti-Mouse ..
Polyclonal Anti-N-cadh..
Polyclonal Anti-Rat Bo..
³»¿ë (24ȸ)
1. Lysis Buffer
1.2 §¢¡¿4 tubes
2. 10% SDS Solution
0.48 §¢¡¿1 tube
3. Enzyme A
0.48 §¢¡¿1 tube
4. Enzyme B
0.48 §¢¡¿1 tube
5. Precipitant
1.04 §¢¡¿3 tubes
6. 6¡¿ Loading Buffer
0.48 §¢¡¿1 tube
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Apoptosis´Â chromatin DNAÀÇ nucleosome ´ÜÀ§ (185 bp)·Î ´ÜÆíÈ°¡ ÀϾ´Â °ÍÀÌ Çʼö Á¶°ÇÀÌ´Ù. º» Á¦Ç°Àº Ư¼ö °è¸éÈ°¼ºÁ¦¸¦ ÇÔÀ¯ÇÑ Lysis buffer, È¿¼Ò½Ã¾à, DNA ħÀüÁ¦, Loading Buffer·Î ±¸¼ºµÇ´Â Å°Æ®·Î ´ÜÆíÈ DNA¸¦ ¹è¾ç¼¼Æ÷¿¡¼ ¼±ÅÃÀûÀ¸·Î ÃßÃâÇÒ ¼ö ÀÖ´Ù (¿¡Åº¿Ã ħÀü 󸮱îÁö 2.5½Ã°£ ¼Ò¿ä). º» Á¦Ç°À» »ç¿ëÇÏ¹Ç·Î½á ¹æÇØ°¡ µÇ´Â intact chromatinÀÇ È¥ÀÔÀ» ÃÖ¼ÒÇÑÀ¸·Î ¾ïÁ¦ÇÏ¿© º¸´Ù °í°¨µµ·Î ladderingÀ» °ËÃâÇÒ ¼ö ÀÖ´Ù. ¶Ç, ApopLadder Ex¿Í Çü±¤»ö¼Ò(GelStar : TaKaRa Code 50535, SYBR Green¥°: TaKaRa Code 50512, 50513) ¸¦ Á¶ÇÕÇÏ¿© »ç¿ëÇÏ¸é ´ÜÆíÈµÈ DNA Á¤·®ÀÌ °¡´ÉÇÏ´Ù.
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Apoptosis¸¦ ÀÏÀ¸Å² ¼¼Æ÷¸¦ Lysis Buffer·Î ó¸®ÇÑ ÈÄ ¿ø½ÉºÐ¸®ÇÏ°í »óµî¾×À» ȸ¼öÇÏ¿© ´ÜÆíÈ DNA¸¦ intact chromatinÀ¸·ÎºÎÅÍ ºÐ¸®ÇÑ´Ù. ÀÌ »óµî¾×À» È¿¼Ò ½Ã¾àÀ¸·Î ó¸®ÇÏ¿© ºÒ¼ø¹°ÀÎ ´Ü¹éÁú°ú RNA¸¦ ºÐÇØÇÑ ÈÄ DNA ħÀüÁ¦¸¦ ÀÌ¿ëÇÏ¿© ´ÜÆíÈ DNA¸¦ ºÐ¸®ÇÑ´Ù.
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Keyword :
Apoptosis
ApoAlert Annexin V-FITC Apoptosis Kit
ApoAlert Caspase Assay Kit
ApoAlert pDsRed2-Bid Vector
Apoptosis·Î ÀÎÇÑ ´ÜÆíÈ DNA °ËÃâ
ApopLadder Ex¢â
Bcl-2 À¯ÀüÀÚ RT-PCR¿¡
ApoPrimer Set (Bcl-2 family)
TUNEL¹ýÀ» ÀÌ¿ëÇÑ Apoptosis °ËÃâ
In situ Apoptosis Detection Kit