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miRNA cDNA ÇÕ¼º°ú Á¤·®

MicroRNA qRT-PCR Á¤·®

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Clontech
638314
Mir-X miRNA qRT-PCR TB Green¢ç Kit
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200 ȸ
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Clontech
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Mir-X miRNA qRT-PCR TB Green¢ç Kit
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600 ȸ (638314 x 3)
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Mir-X miRNA First-Strand Synthesis Kit
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20 ȸ
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Clontech
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Mir-X miRNA First-Strand Synthesis Kit
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840,000¿ø
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  • °£ÆíÇÑ ´ÜÀÏ ¹ÝÀÀ ´Ü°èÀÇ cDNA ÇÕ¼º
  • µ¿ÀÏÇÑ RNA½Ã·á·Î microRNA¿Í ±× ¸ñÀûÀ¯ÀüÀÚ¸¦ Á¤·® °¡´É
  • 50 copies±îÁö ÀûÀº ¾çÀÇ microRNAµµ °ËÃâ °¡´É
  • ³ôÀº ƯÀ̼º High specificity
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Mir-X miRNA qRT-PCR TB Green¢â KitsÀº total RNA³ª Á¤Á¦µÈ small RNA ½Ã·á·ÎºÎÅÍ qPCRÀ» ÅëÇØ microRNA¸¦ Á¤·® ÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÌ´Ù. °¢ Å°Æ®¿¡´Â First-strand cDNA synthesisÁ¦Ç°°ú Quantitative PCR (qPCR) Á¦Ç°ÀÌ Æ÷ÇԵǾî ÀÖ´Ù.

(#638314 Á¦Ç° ³»¿¡´Â miRNAÀÇ cDNA ÇÕ¼ºÀ» À§ÇÑ #638313 Á¦Ç°°ú qPCRÀ» À§ÇÑ ½Ã¾àÀ» ¸ðµÎ Æ÷ÇÔÇÏ°í ÀÖ½À´Ï´Ù.
#638316 Á¦Ç° ³»¿¡´Â miRNAÀÇ cDNA ÇÕ¼ºÀ» À§ÇÑ #638315 Á¦Ç°°ú qPCRÀ» À§ÇÑ ½Ã¾àÀ» ¸ðµÎ Æ÷ÇÔÇÏ°í ÀÖ½À´Ï´Ù.)
°£ÆíÇÑ ´ÜÀÏ ¹ÝÀÀÀÇ cDNA ÇÕ¼º
ClontechÀÇ microRNA Á¤·® Å°Æ®´Â °£ÆíÇÑ ´ÜÀÏ ¹ÝÀÀÀ¸·Î 1st strand cDNA¸¦ ÇÕ¼ºÇÑ´Ù. Mir-X Á¦Ç°Àº RNA½Ã·á°¡ Æ÷ÇÔµÈ microRNA·ÎºÎÅÍ cDNA¸¦ ÇÕ¼ºÇϵµ·Ï poly(A) polymerase¿Í SMART MMLV Reverse Transcriptase°¡ ÃÖÀûÈ­µÈ È¿¼Ò¸¦ »ç¿ëÇÑ´Ù.
MicroRNA-Specific Primers¸¦ ÀÌ¿ëÇÑ ¹Î°¨µµ ³ôÀº qPCR ¹ÝÀÀ
ClontechÀÇ TB Green¢â Advantage qPCR Premix¿Í ¿¬±¸ÀÚÀÇ microRNA-specific primer¸¦ ÀÌ¿ëÇÏ¿© qPCR·Î cDNA¸¦ ÁõÆøÇÏ°í Á¤·®ÇÑ´Ù. microRNA target »Ó¸¸ ¾Æ´Ï¶ó º¹¼öÀÇ microRNA Á¾µéÀ» ´ÜÀÏ cDNA ½Ã·á·ÎºÎÅÍ ÇÕ¼º °¡´ÉÇÏ´Ù. ÀÌ Á¦Ç°Àº ÃÖÀú 50 copiesÀÇ microRNA±îÁöµµ °ËÃâÇÒ ¼ö ÀÖ´Ù.

ROX Reference Dye LSR¿Í LMPÀÇ ±¸ºÐ
Mir-X miRNA qRT-PCR TB Green¢â¿¡ Æ÷ÇÔµÈ ROX Reference Dye LSR°ú LMP´Â ´ÙÀ½°ú °°ÀÌ ºÐ·ùÇÏ¿© »ç¿ëÇÑ´Ù.
- ROX Reference Dye LSR : 488 §¬ÀÇ Laser¸¦ Excitation source·Î »ç¿ëÇÏ´Â ÀåÄ¡ ¶Ç´Â high ROX¸¦ ¿ä±¸ÇÏ´Â ÀåÄ¡
- ROX Reference Dye LMP : Lamp ¶Ç´Â LED¸¦ Excitation source·Î »ç¿ëÇÏ´Â ÀåÄ¡ ¶Ç´Â low ROX¸¦ ¿ä±¸ÇÏ´Â ÀåÄ¡
MicroRNAÀÇ Æ¯À̼º ³ôÀº °ËÃâ
¸Å¿ì À¯»çÇÑ Let7 miRNA º¯ÀÌü 8°³¸¦ »ç¿ëÇÏ¿© Mir-X miRNA Á¤·®ÀÇ Æ¯À̼º¿¡ ´ëÇÑ ½ÇÇèÀ» ½Ç½ÃÇÏ¿´´Ù. Mir-X single-tube ¹ÝÀÀÀ¸·Î ÇÕ¼ºµÈ cDNA¿Í yeast polyA+ RNA·ÎºÎÅÍ ºÐ¸®µÈ ½Ã·á °¢°¢¿¡ Let7 miRNAs¸¦ ÷°¡ÇÏ¿´´Ù. ±×·± ÈÄ °¢°¢ÀÇ cDNA ½Ã·á¸¦ variant-specific primers¸¦ ÀÌ¿ëÇÏ¿© Let7 subtypesÀÇ Á¤·® Å×½ºÆ®¸¦ ÇÏ¿´´Ù. º¯Á¾°ú primer »çÀÌ¿¡ ³ôÀº À¯»ç¼º¿¡µµ ºÒ±¸ÇÏ°í Mir-X qPCRÀº °¢°¢ÀÇ Let7 º¯Á¾À» ƯÀ̼º ³ô°Ô °ËÃâÇÏ¿´´Ù.



Specific quantification of Let7 miRNA variants. Using miRNA-specific primers (Panel A), Mir-X qRT-PCR was able to specifically detect and quantify each member of a series of 8 synthetic Let7 variants that had been spiked into a background of yeast poly(A) + Panel B).The primers detected each of their corresponding Let7 miRNA cognates, but did not detect the off-target variants in 63 of 64 possible combinations.

Mir-X miRNA qRT-PCR TB Green¢â Kits use a single-step, single-tube reaction to produce first-strand cDNA, which is then specifically and quantitatively amplified using a miRNA-specific primer and TB Green¢â Advantage qPCR chemistry. In the Mir-X cDNA synthesis reaction, RNAs are poly(A)-tailed using poly(A) polymerase, and then copied using a modified oligo(dT) primer and SMART MMLV Reverse Transcriptase.


Trichostatin A treatment alters miRNA expression in mouse ES cells. In mouse embryonic stem cells, we were able to monitor the alterations in expression for a panel of 12 miRNAs that respond to trichostatin A (TSA) treatment.

Induction and quantitation of miR-9 miRNA in mouse P19 cells. Exposing aggregated mouse P19 cell clusters to retinoic acid (RA) causes them to acquire neural cell phenotypes, which are accompanied by changes in the cellular miRNA pool. Using the Mir-X miRNA quantitation system, we tracked the abundance of one such miRNA, miR-9, which was induced by RA and continued to accumulate in these cells following a 5 day exposure to RA.

Keyword : miRNA,Mir-X,microRNA,MirX

miRNA ¹ßÇö ¡¤ Á¤·®
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miRNA cDNA ÇÕ¼º°ú Á¤·®
MicroRNA qRT-PCR Á¤·®
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Small RNA (miRNA, siRNA, piRNA, sno RNA µî) ..
Small RNA-Seq Library Prep Kit
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ÆíÇ⼺ ³·°í Á¤È®¼ºÀÌ ³ôÀº, microRNA specific..
MicroRNA-Seq Library Prep Kit