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Epigenetics
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DNA Methlyation ¿¬±¸
> EpiScope¢ç MSP Kit
ƯÁ¤ ¿µ¿ªÀÇ DNA MethylationÀ» Çؼ® °¡´É
EpiScope¢ç MSP Kit
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Takara
R100A
EpiScope¢ç MSP Kit
200 ȸ
602,400¿ø
753,000¿ø
09.02 ~ 10.31
Takara
R100B
EpiScope¢ç MSP Kit
400 ȸ (R100A x 2)
1,430,000¿ø
Product index
Overview
[¼±Åð¡À̵å] Epigeneti..
[°³¿ä] ChIP-Seq Librar..
Cancer ¿¬±¸ °¡À̵å
DNA Methlyation ¿¬±¸
Metly-sensitive/non-se..
TaKaRa EpiTaq HS (for ..
EpiScope¢ç MSP Kit
TB Green¢ç Premix Ex T..
¸ÞÆ¿È/ºñ¸ÞÆ¿È ÄÁÆ®·Ñ..
McrBC
Methylated DNA ºÐ¸®¿Í ºÐ¼®
EpiXplore Methylated D..
Meth-Seq DNA Enrichmen..
Chromatin ¸é¿ª ħ°¨ (ChIP)
Micrococcal Nuclease
Anti-Histone monoclona..
EpiXplore ChIP Assay K..
DNA Fragmentation Kit
ChIP Elute Kit
EpiScope¢ç Nucleosome ..
Capturem¢â IP £¦ Co-IP..
ChIP-seq, Cut&Run-seq (NGS..
[Àû¿ë] ¼Ò·®¼¼Æ÷·ÎºÎÅÍ ..
[Customer Spotlight] C..
[DNA-Seq ±â¼ú°³¿ä] Thr..
[ChIP-seq ³í¹®¸®½ºÆ®] ..
SMARTer¢ç ThruPLEX¢ç D..
DNA SMART ChIP-Seq Kit
SMARTer¢ç DNA index ki..
miRNA ¹ßÇö ¡¤ Á¤·®
[Cancer Research] Canc..
MicroRNA ¿¬±¸¿ë Á¦Ç° ..
MicroRNA qRT-PCR Á¤·®
Tet Inducible miRNA ¹ß..
Small RNA-Seq Library ..
MicroRNA-Seq Library P..
miRNA ¹ßÇö vector (Çü..
Epigenetics¸¦ À§ÇÑ Cloning..
Online In-Fusion Tools
Mighty TA-cloning Kit
Á¦Ç°¼³¸í
EpiScope MSP Kit´Â MSP (Methylation Specific PCR) Àü¿ë PCR ½Ã¾àÀÌ´Ù. MSP¿¡¼´Â bisulfite 󸮿¡ ÀÇÇØ, CpG ¹è¿ÀÇ ¸ÞÆ¿È¿¡ µû¶ó ¹è¿ÀÌ ¹Ù²î´Â ¿°±â ºÎÀ§¿¡ ¸ÞÆ¿È DNA °ËÃâ¿ë ÇÁ¶óÀ̸Ó(M Primer)¿Í ºñ¸ÞÆ¿È DNA °ËÃâ¿ë ÇÁ¶óÀ̸Ó(UM Primer)¸¦ ¼³°èÇÏ¿© PCR¸¦ ½Ç½ÃÇÑ´Ù. º» Á¦Ç°Àº bisulfite ó¸®µÈ uracilÀ» Æ÷ÇÔÇÑ DNA¸¦ ÁÖÇüÀ¸·Î °íÈ¿À² ÁõÆøÀÌ °¡´ÉÇÑ Àü¿ë È¿¼Ò¿Í ÃÖÀûÈµÈ ¹öÆÛ¿¡ ÀÇÇØ ³ôÀº È¿À²·Î ÁõÆøÀÌ °¡´ÉÇϹǷÎ, Á¾·¡ÀÇ PCR ½Ã¾à°ú ºñ±³ÇØ ¸íÈ®ÇÑ DNA ¸ÞÆ¿È/ºñ¸ÞÆ¿È ¹è¿ ±¸ºÐÀÌ °¡´ÉÇÑ MSP Çؼ®À» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù.
Real Time ¸ð´ÏÅ͸µ¿¡ ÀûÀýÇÑ ³óµµÀÇ TB Green¢â¸¦ Æ÷ÇÔÇÑ »óÅ·Π¹ÝÀÀÀÌ ÃÖÀûÈ µÇ¾î ÀÖ¾î Real Time PCR °ËÃâ(semi-quatitative Çؼ®µµ °¡´É) ¹× end-point °ËÃâ(ÀÏ¹Ý PCRÀ» ÀÌ¿ëÇÑ ¹ÝÀÀÀ¸·Î Àü±â ¿µµ¿À¸·Î ÁõÆøÀ» È®ÀÎ) ¾çÂÊ ¸ðµÎ¸¦ °°Àº ¹ÝÀÀ Á¶°ÇÀ¸·Î °ËÃâÇÒ ¼ö ÀÖ´Ù.
³»¿ë (200 ȸ, 50 ¥ìl ¹ÝÀÀ½Ã)
2¡¿MSP Buffer (Mg
2+
plus, dNTP plus)
*1
1 ml¡¿5°³
MSP Enzyme
240 ¥ìl
100¡¿TB Green¢â
100 ¥ìl
ROX Reference Dye(50¡¿conc.)
*2
200 ¥ìl
ROX Reference Dye II(50¡¿conc.)
*2
200 ¥ìl
*1
Mg
2+
³óµµ´Â 4 mM(2¡¿), dNTP ³óµµ´Â 400 ¥ìM(2¡¿).
*2
Applied Biosystem PCR ÀåÄ¡ µî well °£ÀÇ Çü±¤ ½Ã±×³ÎÀÇ º¸Á¤ÀÌ ÇÊ¿äÇÑ ÀåÄ¡·Î °ËÃâÇÏ´Â °æ¿ì¿¡ »ç¿ëÇÑ´Ù.
Applied Biosystems StepOnePlus Real-Time PCR System¿¡´Â ROX Reference Dye¸¦, 7500 ¹× 7500 Fast Real-Time PCR System¿¡´Â ROX Reference Dye II¸¦ ÀÌ¿ëÇÑ´Ù.
Thermal Cycler Dice Real Time System II(Á¦Ç° ÄÚµå TP900)µîÀÇ Real Time PCR PCR ÀåÄ¡³ª Àü±â ¿µµ¿¿¡ ÀÇÇÑ Çؼ®À» ½Ç½ÃÇÏ´Â Åë»óÀÇ PCR ÀåÄ¡¸¦ »ç¿ëÇÏ´Â °æ¿ì´Â ÇÊ¿ä¾ø´Ù.
º¸Á¸ 20¡É
100¡¿TB Green¢â, ROX Reference Dye, Dye II´Â ¹Ýµå½Ã Â÷±¤ ÇØ ÁÖ¼¼¿ä.
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Real Time PCR °ËÃâ ¹× end-point °ËÃâÀº °°Àº ¹ÝÀÀ¾× Á¶¼º, °°Àº PCR Á¶°ÇÀ¸·Î ¹ÝÀÀÀ» ÁøÇàÇÑ´Ù. end-point °ËÃâÀÇ °æ¿ì¿¡µµ ¹Ýµå½Ã 100¡¿TB Green¢â¸¦ ÷°¡ÇÑ´Ù.
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TB Green¢â´Â ÇÙ»ê°ú °áÇÕÇÏ´Â ¼ºÁú»ó, µ¹¿¬º¯ÀÌÀÇ °¡´É¼ºÀÌ ÀÖ½À´Ï´Ù. ¶Ç, DMSO ¿ë¾×À» Æ÷ÇÔÇÏ°í ÀÖÀ¸¹Ç·Î, Á¶Á÷ Áß¿¡ »ö¼Ò°¡ ħÅõÇÒ ¿ì·Áµµ Àֱ⠶§¹®¿¡ »ç¿ë½Ã ¹Ýµå½Ã Àå°©À» Âø¿ëÇØ ÁÖ¼¼¿ä.
Keyword :
DNA Methlyation ¿¬±¸
Metly-sensitive/non-sensitive Á¦ÇÑÈ¿¼Ò
Bisulfite ó¸® ÈÄÀÇ DNA PCR ÁõÆø¿¡ ÃÖÀû
TaKaRa EpiTaq HS (for bisulfite-treated DNA)
ƯÁ¤ ¿µ¿ªÀÇ DNA MethylationÀ» Çؼ® °¡´É
EpiScope¢ç MSP Kit
genomic DNA ¶Ç´Â CpG island ¿µ¿ªÀÇ ÁõÆø¿¡ ÃÖ..
TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time)
DNA ¸ÞÆ¿È Çؼ® ÄÁÆ®·Ñ
¸ÞÆ¿È/ºñ¸ÞÆ¿È ÄÁÆ®·Ñ °Ô³ð DNA
McrBC