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Epigenetics
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miRNA ¹ßÇö ¡¤ Á¤·®
> MicroRNA-Seq Library Prep Kit
ÆíÇ⼺ ³·°í Á¤È®¼ºÀÌ ³ôÀº, microRNA specific NGS Library Kit
MicroRNA-Seq Library Prep Kit
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Overview
[¼±Åð¡À̵å] Epigeneti..
[°³¿ä] ChIP-Seq Librar..
Cancer ¿¬±¸ °¡À̵å
DNA Methlyation ¿¬±¸
Metly-sensitive/non-se..
TaKaRa EpiTaq HS (for ..
EpiScope¢ç MSP Kit
TB Green¢ç Premix Ex T..
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McrBC
Methylated DNA ºÐ¸®¿Í ºÐ¼®
EpiXplore Methylated D..
Meth-Seq DNA Enrichmen..
Chromatin ¸é¿ª ħ°¨ (ChIP)
Micrococcal Nuclease
Anti-Histone monoclona..
EpiXplore ChIP Assay K..
DNA Fragmentation Kit
ChIP Elute Kit
EpiScope¢ç Nucleosome ..
Capturem¢â IP £¦ Co-IP..
ChIP-seq, Cut&Run-seq (NGS..
[Àû¿ë] ¼Ò·®¼¼Æ÷·ÎºÎÅÍ ..
[Customer Spotlight] C..
[DNA-Seq ±â¼ú°³¿ä] Thr..
[ChIP-seq ³í¹®¸®½ºÆ®] ..
SMARTer¢ç ThruPLEX¢ç D..
DNA SMART ChIP-Seq Kit
SMARTer¢ç DNA index ki..
miRNA ¹ßÇö ¡¤ Á¤·®
[Cancer Research] Canc..
MicroRNA ¿¬±¸¿ë Á¦Ç° ..
MicroRNA qRT-PCR Á¤·®
Tet Inducible miRNA ¹ß..
Small RNA-Seq Library ..
MicroRNA-Seq Library P..
miRNA ¹ßÇö vector (Çü..
Epigenetics¸¦ À§ÇÑ Cloning..
Online In-Fusion Tools
Mighty TA-cloning Kit
³ôÀº ÀçÇö¼º(Reproducibility)°ú ³·Àº ÆíÇ⼺ (bias)
- Çõ½ÅÀûÀÎ ligation ±â¼úÀ» ÀÌ¿ëÇÏ¿© ÆíÇ⼺ÀÌ ³·°í Á¤È®¼ºÀÌ ³ôÀº small RNA (miRNA)-seq library Á¦ÀÛ
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- Total RNA 100ng - 1ug ¶Ç´Â 2 - 200ng enriched small RNA·ÎºÎÅÍ Á¦ÀÛ °¡´É
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SMARTer¢ç microRNA-Seq KitÀº °íÇ°ÁúÀÇ microRNA (miRNA) ºÐ¼®À» À§ÇÑ, Illumina NGS Library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖµµ·Ï °í¾ÈµÈ Á¦Ç°ÀÌ´Ù. µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ MAGIC (
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MAGIC ±â¼úÀº small RNA ¶Ç´Â miRNA¸¦ ĸÃÄÇÏ¿© ÀçÇö¼º ³ô°í Á¤È®ÇÏ°Ô ºÐ¼® °¡´ÉÇÏ°Ô ÇÑ´Ù. º» Á¦Ç°Àº ´ÜÀÏÆ©ºê (single tube)¿¡¼ ÇÏ·ç ¸¸¿¡ (¾à 6½Ã°£) miRNA-Seq library¸¦
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±×¸² 1. SMARTer¢ç microRNA-Seq KitÀÇ MAGIC ±â¼ú °³¿ä
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±×¸² 2. SMARTer¢ç microRNA-seq KitÀ¸·Î microRNA NGS LibraryÀÇ Á¤È®¼º°ú ³ôÀº °ËÃâ´É·Â
MicroRNA libraries generated with the SMARTer microRNA-seq Kit are more accurate and detect more reads than other state-of-the art ligation-based technologies.
Panel A. MicroRNA expression levels (Y axis, log scale) were plotted against microRNA species (X axis, ranked according to expression level).
Panel B. Detected microRNAs (Y axis) were plotted against detection threshold (X axis).
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Ç¥ 1. Table I. Sequencing metrics of RNA species detected from a variety of sources indicate consistent results using the SMARTer microRNA-seq approach.
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Generation of sequencing libraries from small quantities of either total RNA or RNA enriched for small RNA species.
Keyword :
miRNA ¹ßÇö ¡¤ Á¤·®
[Cancer Research] Cancer biomarker quantification
MicroRNA ¿¬±¸¿ë Á¦Ç° °¡À̵å
miRNA cDNA ÇÕ¼º°ú Á¤·®
MicroRNA qRT-PCR Á¤·®
Tet Inducible miRNA ¹ßÇö ½Ã½ºÅÛ
Small RNA (miRNA, siRNA, piRNA, sno RNA µî) ..
Small RNA-Seq Library Prep Kit
ÆíÇ⼺ ³·°í Á¤È®¼ºÀÌ ³ôÀº, microRNA specific..
MicroRNA-Seq Library Prep Kit
miRNA ¹ßÇö vector (Çü±¤ ´Ü¹éÁú Reporter type)