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Home > ÀüÁ¦Ç°º¸±â > Epigenetics > miRNA ¹ßÇö ¡¤ Á¤·® > MicroRNA-Seq Library Prep Kit
ÆíÇ⼺ ³·°í Á¤È®¼ºÀÌ ³ôÀº, microRNA specific NGS Library Kit

MicroRNA-Seq Library Prep Kit

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  • ¾ÆÁÖ ³ôÀº ¹Î°¨µµ (sensitivity) - Total RNA 100ng - 1ug ¶Ç´Â 2 - 200ng enriched small RNA·ÎºÎÅÍ Á¦ÀÛ °¡´É
  • ´ÜÀÏÆ©ºê (Single tube) workflow - 6½Ã°£* ³»¿¡ °íÇ°ÁúÀÇ small RNA library Á¦ÀÛ °¡´É
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SMARTer¢ç microRNA-Seq KitÀº °íÇ°ÁúÀÇ microRNA (miRNA) ºÐ¼®À» À§ÇÑ, Illumina NGS Library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖµµ·Ï °í¾ÈµÈ Á¦Ç°ÀÌ´Ù. µ¶ÀÚÀûÀ¸·Î °³¹ßÇÑ MAGIC (Mono-Adapter liGation and Intramolecular Circularization) ±â¼úÀ» È°¿ëÇÏ¿© 100ng - 1ug total RNA ¶Ç´Â 2 - 200ng enriched small RNA·ÎºÎÅÍ ±âÁ¸ÀÇ ligation ¹æ¹ý ´ëºñ ÆíÇ⼺ÀÌ ¾ÆÁÖ ³·Àº library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù.
MAGIC ±â¼úÀº small RNA ¶Ç´Â miRNA¸¦ ĸÃÄÇÏ¿© ÀçÇö¼º ³ô°í Á¤È®ÇÏ°Ô ºÐ¼® °¡´ÉÇÏ°Ô ÇÑ´Ù. º» Á¦Ç°Àº ´ÜÀÏÆ©ºê (single tube)¿¡¼­ ÇÏ·ç ¸¸¿¡ (¾à 6½Ã°£) miRNA-Seq library¸¦
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±×¸² 1. SMARTer¢ç microRNA-Seq KitÀÇ MAGIC ±â¼ú °³¿ä (ÀÚ¼¼È÷ º¸±â)



±×¸² 2. SMARTer¢ç microRNA-seq KitÀ¸·Î microRNA NGS LibraryÀÇ Á¤È®¼º°ú ³ôÀº °ËÃâ´É·Â
MicroRNA libraries generated with the SMARTer microRNA-seq Kit are more accurate and detect more reads than other state-of-the art ligation-based technologies.
Panel A. MicroRNA expression levels (Y axis, log scale) were plotted against microRNA species (X axis, ranked according to expression level).
Panel B. Detected microRNAs (Y axis) were plotted against detection threshold (X axis).
(µ¥ÀÌÅÍ ÀÚ¼¼È÷ º¸±â)

Ç¥ 1. Table I. Sequencing metrics of RNA species detected from a variety of sources indicate consistent results using the SMARTer microRNA-seq approach.
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Generation of sequencing libraries from small quantities of either total RNA or RNA enriched for small RNA species.

Keyword :

miRNA ¹ßÇö ¡¤ Á¤·®
>
miRNA cDNA ÇÕ¼º°ú Á¤·®
MicroRNA qRT-PCR Á¤·®
>
Small RNA (miRNA, siRNA, piRNA, sno RNA µî) ..
Small RNA-Seq Library Prep Kit
>
ÆíÇ⼺ ³·°í Á¤È®¼ºÀÌ ³ôÀº, microRNA specific..
MicroRNA-Seq Library Prep Kit