´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > Cloning °ü·Ã > Competent Cells & Electro-Cells > Agrobacterium tumefaciens LBA4404 Electro-Cells
½Ä¹° ÇüÁúÀüȯÀ» À§ÇÑ À¯ÀüÀÚ µµÀÔ¿¡

Agrobacterium tumefaciens LBA4404 Electro-Cells

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
9115
Agrobacterium tumefaciens LBA4404 Electro-Cells
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç° 
40 ¥ìl¡¿5
326,400¿ø 
408,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9115_e.v2003Da.pdf

* Takara Plant ½ÇÇè °ü·ÃÁ¦Ç° overview (Ŭ¸¯)
Á¦Ç°¼³¸í
Agrobacterium tumefaciens (Rhizobium radiobactor)´Â Ti-plasmidÀÇ ÀϺÎÀÎ T-DNA (transfer DNA)¸¦ ¼÷ÁÖ ½Ä¹°ÀÇ ¼¼Æ÷ ¾ÈÀ¸·Î À̵¿½ÃÄÑ ½Ä¹° ¿°»öü DNA¿¡ »ðÀÔÇÑ ÈÄ, T-DNA »óÀÇ À¯ÀüÀÚ¸¦ ¹ßÇö½ÃÅ´À¸·Î½á °¨¿° ºÎÀ§¿¡ ±ÙµÎ ¾ÏÁ¾ (Crow gall)À» Çü¼ºÇÑ´Ù.

ÀÌ ±âÀüÀ» È°¿ëÇØ T-DNAÀÇ º´¿ø¼º À¯ÀüÀÚ¸¦ Á¦°ÅÇÏ°í selective marker gene°ú exogenous target gene¸¦ cloning ÇÏ´Â °ÍÀ¸·Î ¼÷ÁÖ ½Ä¹° ¼¼Æ÷ ÇÙÀÇ DNA¿¡ ¿Ü·¡ À¯ÀüÀÚ¸¦ »ðÀÔÇÏ°í (Agrobacterium-mediated gene transfer), ½Ä¹°Ã¼¸¦ ÇüÁúÀüȯÇÏ´Â binary vector¹ýÀÌ °í¾ÈµÇ¾ú´Ù.

Agrobacterium tumefaciens LBA4404 ±ÕÁÖ´Â binary vector¹ýÀÇ °³¹ßÀÚÀÎ ³×´ú¶õµåÀÇ ¶óÀ̵§ ´ëÇÐÀÇ P. JJHooykaas ±³¼ö ±×·ìÀÌ °³¹ßÇÑ ±ÕÁÖÀÌ´Ù. ÀÌ ±ÕÁÖ´Â T-DNAÀÇ vir region (vir geneÀÇ À¯µµ¿Í T-DNA transfer¿¡ °ü¿©)À» °¡Áø pAL4404 Çö󽺹̵带 Æ÷ÇÔÇÏ°í ÀÖ´Ù. º» Á¦Ç°Àº T-DNA¸¦ »ç¿ëÇÑ ½Ä¹° ¼¼Æ÷ ÇüÁúÀüȯ¿¡ ³Î¸® »ç¿ëµÇ´Â ±ÕÁÖÀÌ´Ù.
³»¿ë (20ȸ)

Agrobacterium tumefaciens LBA4404 Electro-Cells

40 §¡ x5

pRI 900 DNA (1 ng/§¡) *1

10 §¡

SOC Medium*2

1 §¢ x10

*1pRI 909 DNA (Code 3261)¿¡¼­ MCS¸¦ Á¦¿ÜÇÑ plasmid ÀÌ´Ù.
*2 SOC MediumÀÇ Á¶¼º

2 %
 

Tryptone

0.5 %

Yeast extract

10 mM

NaCl

2.5 mM

KCl

10 mM

MgSO4

10 mM

MgCl2

20 mM

Glucose

[ÁÖÀÇ]
SOC MediumÀ» ³ì¿´À» ¶§ µå¹°°Ô ħÀüÀÌ »ý±æ ¼ö ÀÖÀ¸³ª Ç°Áú¿¡´Â ¹®Á¦°¡ ¾ø´Ù.
ħÀü¹°ÀÌ »ý±ä, °æ¿ì ½Ç¿Â¿¡¼­ ¼¯°Å³ª 37¡É¿¡¼­ ¸î ºÐ°£ µ¥¿ö¼­ ħÀüÀ» ¿ëÇؽÃŲ ÈÄ »ç¿ëÇÑ´Ù.
º¸Á¸ -80¡É
ÁÖÀÇ: ÀûÀýÇÑ ¿Âµµ¿¡¼­ º¸°üµÇÁö ¾ÊÀº °æ¿ì ÇüÁú Àüȯ È¿À²ÀÌ ¶³¾îÁú ¼ö ÀÖ´Ù.
ÀÌ ¿ÜÀÇ Á¶°Ç¿¡¼­ º¸°üµÈ °æ¿ì, Á¦Ç° ³»¿¡ Æ÷ÇÔµÈ pRI 900 DNA¸¦ ÀÌ¿ëÇØ ÇüÁúÀüȯ È¿À²À» È®ÀÎ ÈÄ »ç¿ëÇÑ´Ù.
¾×ü Áú¼Ò¿¡´Â º¸°üÇÏÁö ¾Ê´Â´Ù.
¿ëµµ
- Transgenic recombinant ½Ä¹° Á¦ÀÛ
- ½Ä¹°·ÎÀÇ T-DNA transfer
- Binary vector delivery
- °íÈ¿À² plasmid transformation
ÇüÁúÀüȯ È¿À²
»ç¿ëÀÚ ¸Þ´º¾ó¿¡ µû¶ó 1 ngÀÇ pRI 900 DNA¸¦ ÇüÁúÀüȯ ÈÄ kanamycin°ú streptomycinÀ» Æ÷ÇÔÇÑ plate¿¡¼­ selection ÇÑ °á°ú, > 5 x 106 colonies/§¶ÀÇ È¿À²À» ¾ò¾ú´Ù.
°ü·ÃÁ¦Ç°
- ½Ä¹° ÇüÁúÀüȯ¿ë °í¹ßÇö T-DNA vector pRI 201-AN DNA (Code 3264 ¿Ü)
- ÃßÃâÀÌ ¾î·Á¿î ½Ä¹° »ùÇÿ¡¼­µµ °í¼øµµ RNA Á¤Á¦ TaKaRa MiniBEST Plant RNA Extraction Kit (Code 9769A)
- ÃßÃâÀÌ ¾î·Á¿î ½Ä¹° »ùÇÿ¡¼­µµ °í¼øµµ DNA Á¤Á¦ TaKaRa MiniBEST Plant Genomic DNA Extraction Kit (Code 9768A)
- Takara Plant ½ÇÇè °ü·ÃÁ¦Ç° overview (Ŭ¸¯)

Keyword :

Competent Cells & Electro-Cells
>
½Ä¹° ÇüÁúÀüȯÀ» À§ÇÑ À¯ÀüÀÚ µµÀÔ¿¡
Agrobacterium tumefaciens LBA4404 Electro-Cells
>
Chaperone teamÀ» ¹ßÇöÇÏ´Â ´ëÀå±Õ BL21 ±ÕÁÖÀÇ..
Chaperone Competent Cell BL21 ½Ã¸®Áî
>
In-Fusion¢ç ½Ã½ºÅÛ¿¡ ÃÖÀûÀÎ comp. Cells
E. coli Competent Cells (Clontech Á¦Ç°)
>
Long strand DNAµµ methylated DNAµµ OK£¡°íÈ¿..
E. coli Competent Cells (Takara Bio Á¦Ç°)