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Afl II

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
1236A
Afl II
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 U
132,000¿ø 
165,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
1236A_DS.v1810Da.pdf
Takara
1236B
Afl II
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,000 U (1236A x 5)
745,000¿ø 

º¸Á¸
-20¡É
³óµµ
10 U/§¡
÷ºÎ ¹× È°¼º ÃøÁ¤ buffer
M buffer + BSA
[Âü°í] Universal Buffer ¡¤ Basal Buffer¿¡ ÀÇÇÑ Á¦ÇÑÈ¿¼Ò È°¼º Ç¥½Ã ½Ã½ºÅÛ
¹ÝÀÀ ¿Âµµ
37¡É
È°¼ºÀ» ÃøÁ¤ÇÑ ±âÁú
¥ëDNA
±â¿ø
Escherichia coli carrying the plasmid encoding Afl IIgene
Star È°¼º
°í³óµµÀÇ ±Û¸®¼¼·Ñ, DMSO ¶Ç´Â Mn2+ °¡ Á¸ÀçÇÒ ¶§ ¼­¿­ ÀÎ½Ä Æ¯À̼ºÀÌ °¨¼ÒµÉ ¼ö ÀÖ´Ù.
Ligation-Recutting Test
º» È¿¼Ò¿¡ ÀÇÇØ »ý±ä µ¹Ãâ ¸»´ÜÀº ÀϹÝÀûÀÎ 4¿°±â µ¹Ãâ ¸»´Üº¸´Ù ligation È¿À²ÀÌ ³·´Ù. ÀÌ ¶§¹®¿¡ ÆòÈ° ¸»´ÜÀÇ ligation°ú µ¿µîÇÑ ¹ÝÀÀ Á¶°ÇÀÌ ÇÊ¿äÇÏ´Ù.
°ü·ÃÁ¦Ç°




Keyword : 1003A,1003,1003B