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Home > ÀüÁ¦Ç°º¸±â > Cloning °ü·Ã > DNA Ligation > TaKaRa DNA Ligation Kit LONG
10 kb ÀÌ»óÀÇ insert cloning¿¡ ÃÖÀû

TaKaRa DNA Ligation Kit LONG

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Takara
6024
TaKaRa DNA Ligation Kit LONG
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1 Kit
237,000¿ø  6024_e.v0704.pdf

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TaKaRa DNA Ligation Kit LONGÀº ±ä ´ÜÆíÀÇ insert DNA¸¦ ³ôÀº È¿À²·Î ligation Çϱâ À§ÇØ °³¹ßµÈ Á¦Ç°À¸·Î,
DNA ligase ¹× ¹ÝÀÀ buffer°¡ ±ä ´ÜÆíÀÇ DNA ligation¿¡ ÃÖÀûÈ­ µÇ¾î ÀÖ´Ù.
ƯÈ÷ 10 kb ÀÌ»óÀÇ insert DNA¸¦ ÀÌ¿ëÇÑ ligation¿¡ À¯¿ëÇϸç, BAC library µî ±ä ´ÜÆíÀ» Æ÷ÇÔÇÏ´Â DNA library Á¦ÀÛ¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
Å©±â°¡ Å« plasmid DNAÀÇ ÇüÁúÀüȯ¿¡ ÃÖÀûÀÎ E. coli HST08 Premium Competent Cells (Code 9128) ¶Ç´ÂE. coli HST08 Premium Electro-Cells (Code 9028)°ú Á¶ÇÕÇؼ­ »ç¿ëÇÔÀ¸·Î½á
10 kbÀÌ»óÀÇ ±æÀÌ°¡ ±ä DNA´ÜÆíÀÇ º¹Á¦³ª library Á¦ÀÛµµ ½±°Ô ÇÒ ¼ö ÀÖ´Ù.
³»¿ë (50 ȸ*)

1.

DNA Ligase <LONG>

50 §¡

2.

10¡¿LONG Ligation Buffer

300 §¡

3.

Control Vector (pUC118/Hind III/BAP)

10 §¡

4.

Control Insert DNA/Hind III (18 kb)

30 §¡

5.

dH2O

1 §¢¡¿ 2

* 50 §¡ ¹ÝÀÀ·® ±âÁØ
: Sticky end ligation ÀÇ °æ¿ì´Â 50 ȸ, blunt end ligation ÀÇ °æ¿ì´Â 10 ȸ ºÐ·®ÀÓ
º¸Á¸ -20¡É
°ü·ÃÁ¦Ç°
- High Fidelity PCR È¿¼Ò
      PrimeSTAR¢ç GXL DNA Polymerase (Code R050A/B)
      PrimeSTAR¢ç GXL Premix (Code R051A/B)
- PCR »ê¹° / plasmid / agarose gel Á¤Á¦
      TaKaRa MiniBEST DNA Fragment Purification Kit (Code 9761A/B)
      TaKaRa MiniBEST Plasmid Purification Kit (Code 9760A/B)
      TaKaRa MiniBEST Agarose Gel DNA Extraction Kit (Code 9762A)
- °íÈ¿À² Competent Cells
      E. coli HST08 Premium Competent Cells (Code 9128)
      E. coli HST08 Premium Electro-Cells (Code 9028)
- Colony PCR¿ë dye plus premix
      EmeraldAmp¢ç GT PCR Master Mix (Code RR310A/B)

*Cloning ½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â Cloning Kit - ¼±Åð¡ÀÌµå ¹Ù·Î°¡±â (Ŭ¸¯)

Keyword : Long fragment,T4 DNA ligase,ligase,cloning,¶óÀÌ°ÔÀÌÁî,Ŭ·Î´×

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10 kb ÀÌ»óÀÇ insert cloning¿¡ ÃÖÀû
TaKaRa DNA Ligation Kit LONG