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Home > ÀüÁ¦Ç°º¸±â > cDNA ÇÕ¼º > Reverse Transcription (Clontech) > µ¿°á°ÇÁ¶Å¸ÀÔ RT Premix
Primer±îÁö Æ÷ÇÔµÈ µ¿°á°ÇÁ¶ÇüÅÂÀÇ Mastermix Reverse Transcriptase

µ¿°á°ÇÁ¶Å¸ÀÔ RT Premix

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
639541
RNA to cDNA EcoDry Premix (Oligo dT)
°ü·ÃÇмú ±¸¸ÅÇϱâ
48 rxns (639543x2)
358,400¿ø 
448,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33024
Clontech
639542
RNA to cDNA EcoDry Premix (Oligo dT)
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 rxns (639543 x 4)
625,600¿ø 
782,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639543
RNA to cDNA EcoDry Premix (Oligo dT)
°ü·ÃÇмú ±¸¸ÅÇϱâ
24 rxns
224,800¿ø 
281,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639544
RNA to cDNA EcoDry Premix (Random Hexamers)
°ü·ÃÇмú ±¸¸ÅÇϱâ
48 rxns (639546 x 2)
358,400¿ø 
448,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33024
Clontech
639545
RNA to cDNA EcoDry Premix (Random Hexamers)
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 rxns (639546 x 4)
625,600¿ø 
782,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639546
RNA to cDNA EcoDry Premix (Random Hexamers)
°ü·ÃÇмú ±¸¸ÅÇϱâ
24 rxns
224,800¿ø 
281,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639547
RNA to cDNA EcoDry Premix (Double Primed)
°ü·ÃÇмú ±¸¸ÅÇϱâ
48 rxns (639549 x 2)
358,400¿ø 
448,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33024
Clontech
639548
RNA to cDNA EcoDry Premix (Double Primed)
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 rxns (639549 x 4)
625,600¿ø 
782,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639549
RNA to cDNA EcoDry Premix (Double Primed)
°ü·ÃÇмú ±¸¸ÅÇϱâ
24 rxns
224,800¿ø 
281,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

RNA to cDNA EcoDry Premix ½Ã¸®Áî
RNA to cDNA EcoDry Premix´Â µ¿°á°ÇÁ¶µÈ master mix·Î 1st strand cDNA¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖ´Â °¡Àå Æí¸®ÇÏ°í È¿À²ÀûÀÎ ¹æ¹ýÀ» Á¦°øÇÑ´Ù. Reverse transcription¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ °¢ tube¿¡ ´ã°Ü ÀÖ¾î DW¿Í RNA¸¸ ³ÖÀ¸¸é °ð¹Ù·Î ¹ÝÀÀÀ» ½ÃÀÛÇÒ ¼ö ÀÖ´Ù.
ÇÁ¸®¹Ì¾ö È¿¼Ò
RNA to cDNA EcoDry Premix´Â °í¼øµµÀÇ SMART™ MMLV Reverse Transcriptase¸¦ ±â¹ÝÀ¸·Î ÇÏ°í ÀÖÀ¸¸ç °íÇ°ÁúÀÇ 1st strand cDNA¸¦ 11.7kb±îÁö ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù. microarray probe ÇÕ¼ºÀ̳ª real-time quantitative RT-PCR µîÀÇ »ç¿ë¿¡ ÀÌ»óÀûÀ̸ç À¯ÀüÀÚ cloningÀ̳ª RACE ¶Ç´Â ÀϹÝÀûÀÎ cDNA ÇÕ¼º°ú library ±¸ÃàÀÌ ÀûÇÕÇÏ´Ù.
ȯ°æ°ú ¿¬±¸ÀÚ¸¦ °í·ÁÇÑ Ä£È¯°æ Á¦Ç° (Eco-Friendly Format)
EcoDry Premix´Â µ¿°á°ÇÁ¶ ÇüÅÂÀÇ ½Ç¿Â¿¡¼­µµ ¾ÈÁ¤ÀûÀÎ Á¦Ç°À¸·Î, ¿î¹Ý½Ã ½ºÆ¼·ÎÆû ¹Ú½º³ª ³Ãµ¿°í µîÀÌ ÇÊ¿äÇÏÁö ¾ÊÀ¸¸ç ¶ÇÇÑ master mix Á¦Ç°À̱⠶§¹®¿¡ ½ÇÇè °úÁ¤½Ã ¹ß»ýµÇ´Â tube, tip, ±×¿Ü ¿©·¯°¡Áö Çöó½ºÆ½ Á¦Ç°ÀÇ ¼Òºñ¸¦ ÁÙÀÏ ¼ö ÀÖ´Ù. ÀÌ´Â ¿¬±¸ÀÚÀÇ ¼Ò¸ðÇ° ºñ¿ë Àý°¨°ú ¼Â¾÷ ½Ã°£À» Àý¾à ÇÒ ¼ö ÀÖÀ¸¸ç ¶ÇÇÑ ¾²·¹±â¿Í CO2 ¹ß»ýÀ» ÁÙÀÏ ¼ö Àִ ģȯ°æÀûÀÎ Á¦Ç°ÀÌ´Ù.
½Ç¿Âº¸Á¸ °¡´ÉÇÑ µ¿°á°ÇÁ¶ÇüÅÂ
»ç¿ëÀÌ Æí¸®ÇÑ master mix ÇüÅ´ ½ÇÇè ¹ÝÀÀ ¼Â¾÷ ½Ã°£À» ÁÙÀÌ°í ½ÇÇè °úÁ¤»ó ¿À¿° °¡´É¼ºÀ̳ª. pipetting error¸¦ ÁÙÀÏ ¼ö ÀÖ´Ù. ¶ÇÇÑ ½Ç¿Â¿¡¼­ ¾ÈÁ¤ÀûÀ̹ǷΠÇöÀå¿¡¼­ ½ÇÇèÀ» ÁøÇàÇØ¾ß ÇÒ °æ¿ì ¸Å¿ì Æí¸®ÇÑ Á¦Ç°À̶ó°í ÇÒ ¼ö ÀÖ´Ù.



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Reverse Transcription (Clontech)
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Primer±îÁö Æ÷ÇÔµÈ µ¿°á°ÇÁ¶ÇüÅÂÀÇ Mastermix R..
µ¿°á°ÇÁ¶Å¸ÀÔ RT Premix
>
GPR Ultra-Pure MMLV Reverse Transcriptase fo..
MMLV Reverse Transcriptase, GPR
>
Ultra-Pure MMLV RTase
SMART MMLV Reverse Transcriptase