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Cloning °ü·Ã
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In-Fusion Cloning
> [NEW] µ¿°á°ÇÁ¶ In-Fusion¢ç Snap Assembly
½Ç¿Â º¸°ü Master mix
[NEW] µ¿°á°ÇÁ¶ In-Fusion¢ç Snap Assembly
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Clontech
638954
In-Fusion¢ç Snap Assembly EcoDry¢â Master Mix
8 ȸ
(Package)
253,600¿ø
317,000¿ø
11.01 ~ 12.27
,
Clontech
638955
In-Fusion¢ç Snap Assembly EcoDry¢â Master Mix
32 ȸ
(Package)
779,200¿ø
974,000¿ø
11.01 ~ 12.27
Clontech
638956
In-Fusion¢ç Snap Assembly EcoDry¢â Master Mix (96 well plate)
96 ȸ
(Package)
1,724,000¿ø
2,155,000¿ø
11.01 ~ 12.27
,
Clontech
636763
Stellar¢â Competent Cells
10 ȸ (10 x 100ul)
234,400¿ø
293,000¿ø
11.01 ~ 12.27
Clontech
636766
Stellar¢â Competent Cells
50 ȸ (636763 x 5)
996,800¿ø
1,246,000¿ø
11.01 ~ 12.27
Clontech
636767
Stellar¢â Competent Cells (96 well plate)
96 ȸ (96 x 20ul)
(Package)
657,600¿ø
822,000¿ø
11.01 ~ 12.27
Product index
½Ä¹°¿ë Vectors
Takara Plant ½ÇÇè °ü·Ã..
Agrobacterium tumefaci..
pRI 101 DNA ½Ã¸®Áî
pRI 201 DNA ½Ã¸®Áî
pRI 909/910 DNA
È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
pAUR101 DNA
pAUR112 DNA
pAUR123 DNA
pAUR135 DNA
pAUR224 DNA
pAUR316 DNA
pPTR I DNA
pPTR II DNA
Genomic DNA
Genomic DNA
Thermus thermophilus H..
¥ëDNA
¥õX174 RF I DNA
DNA Ligation
½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â..
DNA Blunting Kit
DNA Ligation Kit
DNA Ligation Kit £¼Mig..
TaKaRa DNA Ligation Ki..
TA-Cloning, ÆòÈ°¸»´Ü Cloni..
½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â..
DNA Fragmentation Kit
Kilo-Sequence ¿ë Delet..
Mighty Cloning Reagent..
Mighty TA-cloning Kit
Mighty TA-cloning Reag..
TaKaRa LA PCR¢â in vit..
TaKaRa T-Vector pMD20
In-Fusion Cloning
[Overview] In-Fusion¢ç..
[°³¿ä] So Easy Product
Online Tools for In-Fu..
[NEW] In-Fusion¢ç Snap..
[NEW] µ¿°á°ÇÁ¶ In-Fusi..
[Kit] In-Fusion¢ç Clon..
In-Fusion¢ç Ready Vect..
Genomic DNA ÁõÆø¿ë Kit
GenomeWalkerKit
Vector (Cloning ¿ë)
M13 mp18 Single Strand..
pBR322 DNA
pKF18k-2/19k-3 DNA
pSTV28/29 DNA
pUC118/119 type vector
pUC18/19 type vector
TaKaRa T-Vector pMD20
Competent Cells & Electro-..
Agrobacterium tumefaci..
Chaperone Competent Ce..
E. coli Competent Cell..
E. coli Competent Cell..
E. coli Electro-Cells ..
¼ö½ÄÈ¿¼Ò¿¡ ´ëÇÏ¿©
¼ö½ÄÈ¿¼Ò ¼øµµ À϶÷
¼ö½ÄÈ¿¼Òº° ¿ëµµ
¼ö½ÄÈ¿¼Ò£¯Ligase
E. coli DNA Ligase
T4 DNA Ligase
T4 RNA Ligase
¼ö½ÄÈ¿¼Ò£¯Alkaline Phospha..
Alkaline Phosphatase (..
Alkaline Phosphatase (..
Alkaline Phosphatase (..
T4 Polynucleotide Kina..
¼ö½ÄÈ¿¼Ò£¯DNA Polymerase¡¢..
DNA Polymerase I (E. ..
Klenow Fragment (Large..
PrimeCap¢â T7 RNA Poly..
T4 DNA Polymerase
Terminal Deoxynucleoti..
¼ö½ÄÈ¿¼Ò£¯Nuclease¡¢Topois..
BAL 31 Nuclease
DNA Topoisomerase I
Exonuclease I
Exonuclease III
Micrococcal Nuclease
Mung Bean Nuclease
NucleoGone¢â Endonucle..
Recombinant DNase I (R..
Ribonuclease H (RNase ..
S1 Nuclease
Nucleotide
Deoxyribonucleoside 8..
dNTP Mixture
¹èÁö ¡¤ Buffer (ºÐ¸»)
AccuGENE 20¡¿SSPE Buff..
Bovine Serum Albumin S..
IPTG£¨dioxane free£©£¨..
X-Gal£¨5-Bromo-4-Chlor..
±× ¿Ü (À¯Àü°øÇÐ °ü·Ã)
DNA-OFF¢ç
Proteinase K
Westase (È¿¸ð ¼¼Æ÷º® ..
Yatalase
Yatalase -Plus-
¡ØÂü°í»çÇ×
Ÿ»ç ´ëºñ ¶Ù¾î³ Á¤È®µµ¸¦ °¡Áø In-Fusion
¢ç
ÀÇ ÀÚ¼¼ÇÑ Â÷ÀÌÁ¡Àº ´ÙÀ½ ÀڷḦ Âü°íÇϼ¼¿ä!
>>Gibson¡¯s method¿Í ¿ø¸®ºÎÅÍ °á°ú±îÁö ´Ù¸¥ In-Fusion
¢ç
Snap
»ç¿ë Æí¸®¼º
- 1ȸ »ç¿ë·®ÀÌ µ¿°á°ÇÁ¶ »óÅ·ΠÁ¦°øµÇ¾î ½Ç¿Â¿¡¼ º¸°ü °¡´ÉÇÏ°í, handling ¿À·ù³ª ¿À¿° °¡´É¼ºÀÌ ³·¾Æ ½ÇÇè¾çÀÌ ¸¹À» ¶§ ºü¸£°Ô °á°ú¸¦ ¾òÀ» ¼ö ÀÖÀ½.
Subcloning ºÒÇÊ¿ä
- Cloning À§Ä¡, insert ¼¿¿¡ °ü°è¾øÀÌ, ¸ñÀû vector¿¡ ¹Ù·Î cloning
³ôÀº È¿À²
- 0.5~15 kbÀÇ ´Ù¾çÇÑ Å©±âÀÇ fragment¿¡¼ 95% ÀÌ»óÀÇ Á¤È®µµ
Seamless construction
- ºÒÇÊ¿äÇÑ ¿°±â Ãß°¡ ¾øÀÌ cloning ¿Ï·á (e.g. Á¦ÇÑÈ¿¼Ò »ç¿ë ½Ã, TA cloning ½Ã)
´Ù¾çÇÑ application
- Single insert cloningÀº ¹°·Ð multiple cloning, mutagenesis µî Àû¿ë °¡´É (
´õ ¸¹Àº Àû¿ë »ç·Ê¸¦ º¸½Ã·Á¸é Ŭ¸¯Çϼ¼¿ä
)
Á¦Ç°¼³¸í
In-Fusion
¢ç
Snap Assembly EcoDry¢âÀº cloning ½Ã¾àÀÌ tube³ª well-plate ³»¿¡ 1ȸ¾¿ ºÐÁÖ, µ¿°á°ÇÁ¶µÈ ÇüÅ·Î, ½Ç¿Â¿¡¼ º¸°ü °¡´ÉÇϱ⠶§¹®¿¡ liquid handler¿Í °°Àº ÀÚµ¿È ±â±â¿¡¼ high-throughput cloningÀ» ¸Å¿ì Æí¸®ÇÏ°Ô ÁøÇàÇÒ ¼ö ÀÖ´Ù. Á÷Á¢ ½ÇÇèÀ» Çϰųª ÀÚµ¿È Àåºñ¸¦ ÀÌ¿ëÇÒ ¶§ ¸ðµÎ¿¡¼ handlingÀ» ÃÖ¼ÒÈ ÇÒ ¼ö ÀÖ¾î ÀÌ·Î ÀÎÇÑ error³ª ¿À¿°À» ¹æÁöÇÒ ¼ö ÀÖ´Ù. »Ó¸¸ ¾Æ´Ï¶ó Liquid typeÀÇ ½Ã¾à°ú ¸¶Âù°¡Áö·Î PCR »ê¹°ÀÇ Á¦ÇÑÈ¿¼Ò 󸮳ª ligation, blunting ¾øÀ̵µ cloningÀ» single stepÀ¸·Î ¿Ï·áÇÒ ¼ö ÀÖ´Ù.
In-Fusion
¢ç
±â¼ú Ư¼º »ó ¾î¶² vectorµç Á¦ÇÑÈ¿¼Ò ÀÚ¸®¿¡ »ó°ü¾øÀÌ, subcloningÀ̳ª º°µµÀÇ Á¶ÀÛ ¾øÀÌ insert¸¦ »ðÀÔÇÒ ¼ö ÀÖÀ¸¸ç, cloning °úÁ¤ÀÌ ¸Å¿ì °£´ÜÇϱ⿡ high-throughputÀ¸·Î ½ÇÇèÀÌ °¡´ÉÇÏ´Ù. Cloning °úÁ¤¿¡¼´Â ¼±ÇüÈÇÑ vector¿Í vectorÀÇ ¾ç ¸»´Ü 15 bp¸¦ ºÎ°¡ÇÑ insert¸¦ D.W¿Í ÇÔ²² In-Fusion
¢ç
Snap Assembly EcoDry¢â¿¡ ÷°¡ÇÏ°Ô µÇ´Âµ¥, ¿Â¶óÀο¡¼ Á¦°øÇÏ´Â In-Fusion
¢ç
primer design toolÀ» ÀÌ¿ëÇϸé, °¢°¢ÀÇ ½ÇÇè¿¡ ¾Ë¸ÂÀº primer¸¦ Æí¸®ÇÏ°Ô µðÀÚÀÎÇÒ ¼ö ÀÖ´Ù.
¹ÝÀÀÀÌ ½ÃÀ۵Ǹé, ¼±ÇüÈ µÈ DNA °¡´ÚÀÇ 3¡¯ end·ÎºÎÅÍ ¿°±â¼¿À» Á¦°ÅÇÏ°í, µÎ °³ÀÇ DNA ´ÜÆí¿¡¼ »óº¸¼¿ °£ÀÇ annealingÀ» ÅëÇØ DNA°¡ °áÇÕ½ÃŲ´Ù. ÀÌ ¹æ¹ýÀº SubcloningÀ̳ª º°µµÀÇ Á¶ÀÛ ¾øÀÌ, ÇϳªÀÇ fragment»Ó ¾Æ´Ï¶ó ¿©·¯ °³ÀÇ fragment¸¦ ÇϳªÀÇ vector¿¡ ¹Ù·Î Àû¿ëÇÒ ¼ö ÀÖ¾î high-throughput ½ÇÇè¿¡ cloningÇÒ ¼ö ÀÖÀ» »Ó¸¸ ¾Æ´Ï¶ó, adapter, linker, protein tag¸¦ »ðÀÔÇϰųª mutagenesis, gene synthesis µîÀÇ application¿¡µµ È°¿ëÇÒ ¼ö ÀÖ´Ù.
Applications
- PCR cloning
- High-throughput cloning (see full range of reagent options on our high-throughput cloning page)
- Multiple-fragment cloning
- Gene synthesis
- Gene design
- Mutagenesis
- Domain swapping
- Domain modification
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
Keyword : infusion,in-fusion,cloning,PCR cloning,cloning,Ŭ·Î´×,º¹¼ö´ÜÆí,ÀÎÇ»Àü,ÇÁ·Î¹Í½º,premix, mutagenesis,mutation,soeasy,so easy,Ecodry,µ¿°á°ÇÁ¶,lyophilized,pre-aliquoted
In-Fusion Cloning
[Overview] In-Fusion¢ç Cloning
[°³¿ä] So Easy Product
Online Tools for In-Fusion
ÃÖ°íÀÇ Colony Çü¼º´É°ú Á¤È®ÇÑ PCR cloning
[NEW] In-Fusion¢ç Snap Assembly
½Ç¿Â º¸°ü Master mix
[NEW] µ¿°á°ÇÁ¶ In-Fusion¢ç Snap Assembly
PremixÈ! ½Ã°£´ÜÃà! È¿À²¼º Çâ»ó!
[Kit] In-Fusion¢ç Cloning
In-Fusion¢ç cloningÀ» ¹Ù·Î ÁøÇàÇϱâ À§ÇÑ lin..
In-Fusion¢ç Ready Vectors