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ÀüÁ¦Ç°º¸±â
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Cloning °ü·Ã
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Vector (Cloning ¿ë)
> pSTV28/29 DNA
pSTV28/29 DNA
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Takara
3331
pSTV28 DNA
25 ¥ìg
(0.5 OD)
100,000¿ø
Takara
3332
pSTV29 DNA
25 ¥ìg
(0.5 OD)
100,000¿ø
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Vector (Cloning ¿ë)
M13 mp18 Single Strand..
pBR322 DNA
pKF18k-2/19k-3 DNA
pSTV28/29 DNA
pUC118/119 type vector
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TaKaRa T-Vector pMD20
Competent Cells & Electro-..
Agrobacterium tumefaci..
Chaperone Competent Ce..
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±× ¿Ü (À¯Àü°øÇÐ °ü·Ã)
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Proteinase K
Westase (È¿¸ð ¼¼Æ÷º® ..
Yatalase
Yatalase -Plus-
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DNA ±æÀÌ
pSTV28/29 : 2,999 bp
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pSTV28/29´Â pACYC184ÀÇ º¹Á¦±âÁ¡, Tn9ÀÇ chloramphenicol ³»¼º À¯ÀüÀÚ¿Í pUC18/19ÀÇ multi-cloning site¸¦ °®°í ÀÖ´Â (´Ü,
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I, Acc I ºÎÀ§´Â »ç¿ëÇÒ ¼ö ¾ø´Ù), ¥â-galactosidase À¯ÀüÀڷκÎÅÍ À籸ÃàÇÑ plasmid vectorÀÌ´Ù. pUC °è¿¡ ºñ±³ÇÏ¿© copy ¼ö°¡ ÀûÀ¸¹Ç·Î ´Ù·®À¸·Î ¹ßÇöÇßÀ» °æ¿ì ¼÷ÁÖ¿¡ À¯ÇØÇÑ À¯ÀüÀÚ¸¦ cloningÇϴµ¥ À¯È¿ÇÏ´Ù. ¶Ç pACYCÀÇ º¹Á¦ ±âÁ¡À» °®°í Àֱ⠶§¹®¿¡ pUC, pBR µîÀÇ plasmid vector¿Í µ¿ÀÏ ±Õü ³»¿¡ °øÁ¸½Ãų ¼ö ÀÖ´Ù.
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- M13 primers¸¦ »ç¿ëÇÑ DNA ¿°±â¼¿ °áÁ¤
- Kilo-Sequence¿ë Deletion Kit (Code 6030)¸¦ »ç¿ëÇÑ ±ä DNA´ÜÆíÀÇ ¿°±â¼¿ °áÁ¤
pSTV28/pSTV29 ÀÇ ±¸Á¶
Keyword :
Vector (Cloning ¿ë)
M13 mp18 Single Strand DNA£¨Virion DNA£©
pBR322 DNA
pKF18k-2/19k-3 DNA
pSTV28/29 DNA
pUC118/119 type vector
pUC18/19 type vector
È¿À² ³ôÀº TA-cloning¿¡
TaKaRa T-Vector pMD20