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Home > ÀüÁ¦Ç°º¸±â > PCR > RT-PCR °ü·Ã Á¦Ç° > TaKaRa RNA LA PCR¢â Kit (AMV) Ver.1.1
Long strand cDNA ÁõÆø¿¡

TaKaRa RNA LA PCR¢â Kit (AMV) Ver.1.1

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR012A
TaKaRa RNA LA PCR¢â Kit (AMV) Ver.1.1
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ȸ
600,800¿ø 
751,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
³³±âÁö¿¬ (2022.7. ±âÁØ)
RR012A_e.v2006Da.pdf

*º» Á¦Ç°Àº ±¸¸Å Àü ¹Ýµå½Ã °í°´Áö¿ø¼¾ÅÍ (02-2081-2510)·Î ³³±â È®ÀÎ ¹Ù¶ø´Ï´Ù.
Á¦Ç°¼³¸í
º» Á¦Ç°Àº RNA¸¦ cDNA·Î º¯È¯ÇÑ ÈÄ¿¡ PCRÀ» ½Ç½ÃÇϱâ À§ÇÑ 2 Step RT-PCR Kit·Î AMV (Avian Myeloblastosis Virus) À¯·¡ÀÇ ¿ªÀü»çÈ¿¼Ò¿¡ ÀÇÇØ RNA·Î ºÎÅÍ cDNA ÇÕ¼º ±×¸®°í, TaKaRa LA Taq¿¡ ÀÇÇÑ cDNAÀÇ ÁõÆøÀ» ÇÑ °³ÀÇ tube ³»¿¡¼­½Ç½ÃÇÒ ¼ö ÀÖ´Ù.
Oligo-dT Adaptor Primer´Â poly(A)+ RNAÀÇ 3¡¢¸»´ÜÀ¸·ÎºÎÅÍ ¾ÆÁÖ ³ôÀº È¿À²·Î cDNA¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖµµ·Ï ¼³°èµÇ¾î ÀÖ´Ù. µû¶ó¼­ 3¡¢- RACE¹ý¿¡ ÀÇÇÑ ¹ÌÁöÀÇ 3¡¯¸»´ÜÀÇ ÁõÆø¿¡ ¿ëÀÌÇÏ´Ù.
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RNA template ¸ðµç RNA
ÁõÆø»çÀÌÁî 12kb¶óÁö ÁõÆø°¡´É
¿ªÀü»çÈ¿¼Ò AMV Reverse Transcriptase XL (42~60¡É¿¡¼­ ¿ªÀü»ç°¡´É)
DNA Polymerase TaKaRa LA Taq
RNase Inhibitor Kit¿¡ Æ÷ÇÔ
1st strand cDNAÇÕ¼º Random 9 mers,
ÇÕ¼º Primer Oligo dT- Adaptor Primer
ƯÀÌÀû ÇÏ·ù primer »ç¿ë°¡´É
3¡¯- RACE¹ý RT ¹ÝÀÀ ½Ã Oligo dT-Adaptor Primer, PCR ¹ÝÀÀ ½Ã M13
M4 Primer¸¦ »ç¿ëÇϹǷνá 3¡¯- RACE¹ýÀÌ °¡´É
Á¶ÀÛ 1°³ÀÇ tube ³»¿¡¼­ ¹ÝÀÀ°¡´É, ¿­Ã³¸®ÇÏ¿© ¿ªÀü»ç È¿¼Ò È°¼ºÀ» ½ÇÈ°½ÃŲ ÈÄ PCRÇÑ´Ù.
³»¿ë (100 ȸ) *1
AMV Reverse Transcriptase XL (5 U/§¡) 50 §¡
RNase Inhibitor (40 U/§¡£© 25 §¡
Random 9 mers (50 pmol/§¡) 50 §¡
Oligo dT-Adaptor Primer (2.5 pmol/§¡) 50 §¡
RNase Free dH2O 1 §¢
TaKaRa LA Taq (5 U/§¡)§¡) 25 §¡
M13 Primer M4 (20¥ìM) 50 §¡
10¡¿ RNA PCR Buffer *2 120 §¡
10¡¿ LA PCR Buffer II (Mg2£« free) 5000 §¡
dNTP Mixture (°¢ 10 mM) 150§¡
MgCl2 (25 mM) 1§¡
Control F-1 Primer (20 pmol/§¡)
(Positive Control RNA downstream primer)
25§¡
Control R-1 Primer (20 pmol/§¡)
(Positive Control RNA upstream primer)
25§¡
Positive Control RNA (2¡¿105 copies/§¡)
(pSPTet3 plasmidÀÇ Àü»ç poly(A)£« RNA)
25§¡

*1 [10 §¡ ¿ªÀü»ç¹ÝÀÀ ¡æ 50 §¡ PCR¹ÝÀÀ]ÀÇ 100ȸºÐ
*2 100mM Tris-HCI (pH8.3), 500mM KCI.
º¸Á¸ -20¡É
°ü·ÃÁ¦Ç°
Reverse Transcriptase XL (AMV) for RT-PCR (TaKaRa Code 2630A)
Ribonuclease Inhibitor (TaKaRa Code 2311A)
Ribonuclease Inhibitor (TaKaRa Code 2310A)
TaKaRa LA Taq (TaKaRa Code RR002A)

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