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Home > ÀüÁ¦Ç°º¸±â > PCR > DNA PCR °ü·Ã Á¦Ç° > TaKaRa LA PCR¢â Kit Ver.2.1

TaKaRa LA PCR¢â Kit Ver.2.1

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR013A
TaKaRa LA PCR¢â Kit Ver. 2.1
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
340,000¿ø 
425,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR013A_e.v1906Da.pdf
Takara
RR013B
TaKaRa LA PCR¢â Kit Ver. 2.1
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ȸ (RR013A x 2)
808,000¿ø 

Á¦Ç°¼³¸í
TaKaRa LA PCR Kit Ver.2.1Àº LA PCR technologyÀÇ È¿°ú¸¦ ÃÖ´ëÇÑ È°¿ëÇÏ¿© ±× À§·ÂÀ» ¹ßÈÖÇϵµ·Ï °³¹ßÇÑ »õ·Î¿î LA PCR KitÀÌ´Ù. LA PCR¿ëÀ¸·Î ÃÖÀûÈ­ÇÑ Àü¿ë buffer (LA PCR Buffer II)¿Í TaKaRa LA Taq ÀÇ Á¶ÇÕÀ¸·Î, human genomic DNA¸¦ templateÀ¸·Î ±ä ´ÜÆíÀÇ DNA¸¦ ÁõÆøÇÏ´Â µî º¸´Ù ±ä ´ÜÆíÀÇ PCRÀ» °¡´ÉÇÏ°Ô ÇÏ´Â PCR KitÀÌ´Ù. ¹°·Ð ´Ù¾çÇÑ template DNA¿¡ ÀÀ¿ëÇÒ ¼ö ÀÖÀ¸¸ç ªÀº ´ÜÆíÀÇ DNA ÁõÆø¿¡µµ »ç¿ëÇÒ ¼ö ÀÖ´Ù. Mg2+ freeÀÇ buffer¸¦ ÷ºÎÇÏ¹Ç·Î½á ¹ÝÀÀ¾× Á¶¼ºÀ» °ËÅäÇÒ ¼ö ÀÖ´Ù.
³»¿ë (50 §¡ PCR 50 ȸ)
TaKaRa LA Taq (5 U/§¡) 125 U
dNTP Mixture (°¢ 2.5 mM) 400 §¡
10 ¡¿ LA PCR Buffer II (Mg2+, plus) (Mg2+ ³óµµ 25 mM) 250 §¡
10 ¡¿ LA PCR Buffer II (Mg2+ free) 250 §¡
MgCl2 (25 mM) 500 §¡
Control Template (100 ng/§¡ HT29 À¯·¡ genome DNA) 10 §¡
Control Primer LA3 (10 ¥ìM) 10 §¡
Control Primer LA4 (10 ¥ìM) 10 §¡
¥ë-Hind III digested MW Marker (100 ng/§¡) 20 §¡
2¡¿GC Buffer I (Mg2+ plus)* (Mg2+ ³óµµ 5 mM) 1.25 §¢
2¡¿GC Buffer II (Mg2+ plus)* (Mg2+ ³óµµ 5 mM) 1.25 §¢
Control Primer GC1 (10 ¥ìM) 10 §¡
Control Primer GC2 (10 ¥ìM) 10 §¡
* ÁõÆø¿µ¿ªÀÌ GC-rich ¶Ç´Â °ß°íÇÑ 2Â÷ ±¸Á¶¸¦ ÃëÇÏ´Â °ÍÀ¸·Î ÆǴܵǴ °æ¿ì, ¿ì¼± 2¡¿GC Buffer IÀ» »ç¿ëÇÑ´Ù. Buffer IÀ¸·Î ÁõÆøÀÌ ¾î·Á¿î °æ¿ì¿¡´Â 2¡¿GC Buffer II¸¦ »ç¿ëÇÏ´Â °æ¿ì ¹ÝÀÀÀÌ °³¼±µÇ´Â °æ¿ì°¡ ÀÖ´Ù.
º¸Á¸ -20¡É
Control Primer ¼­¿­
Control Primer LA3:
5¡¯-ACATGATTAGCAAAAGGGCCTAGCTTGGACTCAGA-3¡¯
Control Primer LA4:
5¡¯-TGCACCTGCTCTGTGATTATGACTATCCCACAGTC-3¡¯
* Control TemplateÀÇ 17.5 kb¸¦ ÁõÆøÇÑ´Ù.

Control Primer GC1:
5¡¯-GGGAGGGGACCGGGGAACAGAG-3¡¯
Control Primer GC2:
5¡¯-GAACAGTCCGTCACTTCACGTG-3¡¯
* Control TemplateÀÇ GC-rich ¿µ¿ª 1,255 bp¸¦ ÁõÆøÇÑ´Ù.

Keyword :

DNA PCR °ü·Ã Á¦Ç°
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