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Cloning °ü·Ã
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In-Fusion Cloning
> [NEW] In-Fusion¢ç Snap Assembly
ÃÖ°íÀÇ Colony Çü¼º´É°ú Á¤È®ÇÑ PCR cloning
[NEW] In-Fusion¢ç Snap Assembly
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
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°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Clontech
638947
In-Fusion¢ç Snap Assembly Master Mix
10 ȸ
234,400¿ø
293,000¿ø
11.01 ~ 12.27
,
Clontech
638948
In-Fusion¢ç Snap Assembly Master Mix
50 ȸ
995,200¿ø
1,244,000¿ø
11.01 ~ 12.27
Clontech
638949
In-Fusion¢ç Snap Assembly Master Mix
250 ȸ
3,236,000¿ø
4,045,000¿ø
11.01 ~ 12.27
Clontech
638943
In-Fusion¢ç Snap Assembly Master Mix
500 ȸ (638949 x 2)
5,911,200¿ø
7,389,000¿ø
11.01 ~ 12.27
Clontech
638944
In-Fusion¢ç Snap Assembly Master Mix
1,000 ȸ (638949 x 4)
11,200,000¿ø
14,000,000¿ø
11.01 ~ 12.27
Clontech
639615
Cloning Enhancer
100 ȸ
314,400¿ø
393,000¿ø
11.01 ~ 12.27
Clontech
638951
In-Fusion¢ç Snap Assembly Master Mix with Competent Cells
10 ȸ
(Package)
424,800¿ø
531,000¿ø
11.01 ~ 12.27
,
,
Clontech
638952
In-Fusion¢ç Snap Assembly Master Mix with Competent Cells
50 ȸ
(Package)
1,955,200¿ø
2,444,000¿ø
11.01 ~ 12.27
Clontech
636763
Stellar¢â Competent Cells
10 ȸ (10 x 100ul)
234,400¿ø
293,000¿ø
11.01 ~ 12.27
Clontech
636766
Stellar¢â Competent Cells
50 ȸ (636763 x 5)
996,800¿ø
1,246,000¿ø
11.01 ~ 12.27
Clontech
636767
Stellar¢â Competent Cells (96 well plate)
96 ȸ (96 x 20ul)
(Package)
657,600¿ø
822,000¿ø
11.01 ~ 12.27
Product index
½Ä¹°¿ë Vectors
Takara Plant ½ÇÇè °ü·Ã..
Agrobacterium tumefaci..
pRI 101 DNA ½Ã¸®Áî
pRI 201 DNA ½Ã¸®Áî
pRI 909/910 DNA
È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
pAUR101 DNA
pAUR112 DNA
pAUR123 DNA
pAUR135 DNA
pAUR224 DNA
pAUR316 DNA
pPTR I DNA
pPTR II DNA
Genomic DNA
Genomic DNA
Thermus thermophilus H..
¥ëDNA
¥õX174 RF I DNA
DNA Ligation
½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â..
DNA Blunting Kit
DNA Ligation Kit
DNA Ligation Kit £¼Mig..
TaKaRa DNA Ligation Ki..
TA-Cloning, ÆòÈ°¸»´Ü Cloni..
½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â..
DNA Fragmentation Kit
Kilo-Sequence ¿ë Delet..
Mighty Cloning Reagent..
Mighty TA-cloning Kit
Mighty TA-cloning Reag..
TaKaRa LA PCR¢â in vit..
TaKaRa T-Vector pMD20
In-Fusion Cloning
[Overview] In-Fusion¢ç..
[°³¿ä] So Easy Product
Online Tools for In-Fu..
[NEW] In-Fusion¢ç Snap..
[NEW] µ¿°á°ÇÁ¶ In-Fusi..
[Kit] In-Fusion¢ç Clon..
In-Fusion¢ç Ready Vect..
Genomic DNA ÁõÆø¿ë Kit
GenomeWalkerKit
Vector (Cloning ¿ë)
M13 mp18 Single Strand..
pBR322 DNA
pKF18k-2/19k-3 DNA
pSTV28/29 DNA
pUC118/119 type vector
pUC18/19 type vector
TaKaRa T-Vector pMD20
Competent Cells & Electro-..
Agrobacterium tumefaci..
Chaperone Competent Ce..
E. coli Competent Cell..
E. coli Competent Cell..
E. coli Electro-Cells ..
¼ö½ÄÈ¿¼Ò¿¡ ´ëÇÏ¿©
¼ö½ÄÈ¿¼Ò ¼øµµ À϶÷
¼ö½ÄÈ¿¼Òº° ¿ëµµ
¼ö½ÄÈ¿¼Ò£¯Ligase
E. coli DNA Ligase
T4 DNA Ligase
T4 RNA Ligase
¼ö½ÄÈ¿¼Ò£¯Alkaline Phospha..
Alkaline Phosphatase (..
Alkaline Phosphatase (..
Alkaline Phosphatase (..
T4 Polynucleotide Kina..
¼ö½ÄÈ¿¼Ò£¯DNA Polymerase¡¢..
DNA Polymerase I (E. ..
Klenow Fragment (Large..
PrimeCap¢â T7 RNA Poly..
T4 DNA Polymerase
Terminal Deoxynucleoti..
¼ö½ÄÈ¿¼Ò£¯Nuclease¡¢Topois..
BAL 31 Nuclease
DNA Topoisomerase I
Exonuclease I
Exonuclease III
Micrococcal Nuclease
Mung Bean Nuclease
NucleoGone¢â Endonucle..
Recombinant DNase I (R..
Ribonuclease H (RNase ..
S1 Nuclease
Nucleotide
Deoxyribonucleoside 8..
dNTP Mixture
¹èÁö ¡¤ Buffer (ºÐ¸»)
AccuGENE 20¡¿SSPE Buff..
Bovine Serum Albumin S..
IPTG£¨dioxane free£©£¨..
X-Gal£¨5-Bromo-4-Chlor..
±× ¿Ü (À¯Àü°øÇÐ °ü·Ã)
DNA-OFF¢ç
Proteinase K
Westase (È¿¸ð ¼¼Æ÷º® ..
Yatalase
Yatalase -Plus-
¡ØÂü°í»çÇ×
Ÿ»ç ´ëºñ ¶Ù¾î³ Á¤È®µµ¸¦ °¡Áø In-Fusion
¢ç
ÀÇ ÀÚ¼¼ÇÑ Â÷ÀÌÁ¡Àº ´ÙÀ½ ÀڷḦ Âü°íÇϼ¼¿ä!
>>Gibson¡¯s method¿Í ¿ø¸®ºÎÅÍ °á°ú±îÁö ´Ù¸¥ In-Fusion
¢ç
Snap
* º» Á¦Ç°Àº
In-Fusion
¢ç
HD Cloning kit (Code 639648)
ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°ÀÔ´Ï´Ù.
Subcloning ºÒÇÊ¿ä
- Cloning À§Ä¡, insert ¼¿¿¡ °ü°è¾øÀÌ, ¸ñÀû vector¿¡ ¹Ù·Î cloning
³ôÀº È¿À²
- 0.5~15 kbÀÇ ´Ù¾çÇÑ Å©±âÀÇ fragment¿¡¼ 95% ÀÌ»óÀÇ Á¤È®µµ
Seamless construction
-ºÒÇÊ¿äÇÑ ¿°±â Ãß°¡ ¾øÀÌ cloning ¿Ï·á (e.g. Á¦ÇÑÈ¿¼Ò »ç¿ë ½Ã, TA cloning ½Ã)
´Ù¾çÇÑ application
- Single insert cloningÀº ¹°·Ð multiple cloning, mutagenesis µî Àû¿ë °¡´É (
´õ ¸¹Àº Àû¿ë »ç·Ê¸¦ º¸½Ã·Á¸é Ŭ¸¯Çϼ¼¿ä
)
Á¦Ç°¼³¸í
In-Fusion
®
Snap Assembly´Â ´Ü 15ºÐ ¹ÝÀÀÀ¸·Î ¼±ÇüÈ vector¿¡ ¾î¶² insert¶óµµ ºÒÇÊ¿äÇÑ ¿°±â Ãß°¡ ¾øÀÌ ¹æÇ⼺ ÀÖ´Â cloningÀÌ °¡´ÉÇÏ´Ù. Insert´Â Á¦ÇÑÈ¿¼Ò 󸮳ª phosphorylation, ȤÀº blunt-end ó¸® µî º°µµÀÇ ½ÇÇè °úÁ¤ÀÌ ÇÊ¿äÇÏÁö ¾Ê´Ù. In-Fusion
®
Snap AssemblyÀÇ È¿À²Àº 95% ÀÌ»óÀ¸·Î, ÃæºÐÇÑ ¼öÀÇ colony¸¦ ¾òÀ» ¼ö ÀÖ¾î ´ë·® ºÐ¼®À» À§ÇÑ scale up¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
In-Fusion
®
Snap Assembly Master Mix´Â ligase ¾øÀ̵µ, insert¿Í ¼±ÇüÈ vector ¾ç ¸»´Ü 15 bpÀÇ »óµ¿ ¿°±â¼¿À» ÀÌ¿ëÇÏ¿© È¿À²ÀûÀ¸·Î Á¤È®ÇÏ°Ô °áÇÕ½ÃŲ´Ù. ¿Â¶óÀο¡¼ Á¦°øÇÏ´Â
In-Fusion
®
primer design tool
À» ÀÌ¿ëÇϸé, °¢°¢ÀÇ ½ÇÇè¿¡ ¾Ë¸ÂÀº primer¸¦ ½±°Ô µðÀÚÀÎÇÒ ¼ö ÀÖ´Ù. ¹ÝÀÀÀÌ ½ÃÀ۵Ǹé, In-Fusion
®
Master Mix´Â ¼±ÇüÈµÈ DNA °¡´ÚÀÇ 3’ ¸»´ÜÀ¸·ÎºÎÅÍ ¿°±â¼¿À» Á¦°ÅÇÏ°í, µÎ °³ÀÇ DNA ´ÜÆí¿¡¼ »óº¸¼¿ °£ÀÇ annealingÀ» ÅëÇØ DNA¸¦ °áÇÕ½ÃŲ´Ù. ÀÌ ¹æ¹ýÀº subcloningÀ̳ª º°µµÀÇ Á¶ÀÛ ¾øÀÌ, ¿©·¯ °³ÀÇ fragment¸¦ ÇϳªÀÇ vector¿¡ ¹Ù·Î Àû¿ëÇÒ ¼ö ÀÖ¾î multiple cloningÀÌ °¡´ÉÇÏ´Ù.
In-Fusion
®
Snap Assembly Master Mix¿¡´Â master mix¿Í control vector, control insert°¡ Æ÷ÇԵǾî ÀÖ¾î cloning È¿À²À» °°ÀÌ È®ÀÎÇÒ ¼ö Àֱ⠶§¹®¿¡ ½ÇÇèÀÇ ½Å·Ú¼º ¶ÇÇÑ È®ÀÎÀÌ °¡´ÉÇÏ´Ù.
Stellar™ Competent Cells (Code 636763 ¿Ü)
˼ In-Fusion
®
½Ã½ºÅÛ¿¡ ÃÖÀûȵǾî ÀÖÀ¸¸ç, In-Fusion
®
Snap Assembly Master Mix with Competent Cells (Code 638951, 638952)¿¡´Â Stellar™ Competent CellsÀÌ Æ÷ÇԵǾî ÀÖ´Ù.
±×¸² 1. In-Fusion
®
Workflow
±×¸² 2. In-Fusion
®
Snap Assembly
¿Í In-Fusion HD (±âÁ¸ Á¦Ç°) °£ÀÇ È¿À² ºñ±³.
In-Fusion
®
Snap Assembly´Â ±âÁ¸ Á¦Ç°¿¡ ºñÇØ Á¤È®µµ´Â À¯ÁöÇϸé¼, colony Çü¼º ´É·ÂÀÌ °³¼±µÇ¾ú´Ù.
Á¦Ç° ¼º´É ºñ±³¸¦ À§ÇØ In-Fusion
®
HD (±âÁ¸ Á¦Ç°)°ú In-Fusion
®
Snap AssemblyÀ» »ç¿ëÇÏ¿© ¼·Î ´Ù¸¥ Å©±âÀÇ inserts (405 bp-1,005 bp) 5°³¸¦ 2.7 kb vector¿¡ cloningÇÏ¿´´Ù. Cloning ¹ÝÀÀÀº °¢°¢ ¼¼ ¹ø ¹Ýº¹ ½ÇÇèÇÏ¿´°í, transformation ÈÄ °¢ ¹ÝÀÀÀ¸·ÎºÎÅÍ »ý¼ºµÈ colony¸¦ °¢ 20°³ ¼±ÅÃÇÏ¿© sanger sequencing ÇÑ °á°ú, In-Fusion
®
Snap Assembly¿¡¼ ³ôÀº Á¤È®µµ¸¦ À¯ÁöÇÏ¸é¼ ±âÁ¸ Á¦Ç°º¸´Ù 4¹è ´õ ¸¹Àº colony¸¦ »ý¼ºÇÏ¿´´Ù. µÎ ½ÇÇè¿¡¼ In-Fusion
®
master mix¸¦ Á¦¿ÜÇÑ primer¿Í ½Ã¾à µîÀº µ¿ÀÏÇÏ°Ô ÁøÇàÇÏ¿´´Ù.
±×¸² 3. In-Fusion
®
Snap Assembly
¿Í NEBuilder HiFi (1).
PCR·Î ¼±ÇüÈÇÑ vector¿¡ single insert¸¦ cloningÇÑ °á°ú, insert Å©±â¿¡ °ü°è¾øÀÌ NEBuilder HiFiº¸´Ù In-Fusion
®
Snap Assembly¿¡¼ ÈξÀ ¸¹Àº colony ¼ö¸¦ º¸¿´°í, 95% ÀÌ»óÀ¸·Î ³ôÀº Á¤È®µµ¸¦ º¸¿´´Ù.
3.8 kb insert (A), 34.2 kb adenovirus insert (B)¸¦ 2.7 kbÀÇ ¼±ÇüÈ º¤ÅÍ¿¡ cloningÇÏ¿´´Ù. Cloning ¹ÝÀÀÀº °¢°¢ ¼¼ ¹ø ¹Ýº¹ ½ÇÇèÇÏ¿´°í, transformation ÈÄ °¢ ¹ÝÀÀÀ¸·ÎºÎÅÍ »ý¼ºµÈ colony¸¦ 20°³ ¼±ÅÃÇÏ¿© Sanger sequencing°ú colony PCR ¹æ¹ýÀ¸·Î cloning Á¤È®µµ¸¦ È®ÀÎÇÏ¿´´Ù. ±× °á°ú, In-Fusion
®
Snap Assembly°¡ NEBuilder HiFi¿¡ ºñÇØ 2¹è ´õ ¸¹Àº colony¸¦ »ý¼ºÇÏ¿´´Ù.
±×¸² 4. In-Fusion
®
Snap Assembly¿Í NEBuilder HiFi (2).
Á¦ÇÑÈ¿¼Ò·Î ¼±ÇüÈÇÑ vector¿¡ single insert¸¦ cloningÇÑ °á°ú, ¸»´ÜÀÇ ÇüÅ (overhangs, blunt)¿¡ °ü°è¾øÀÌ NEBuilder HiFiº¸´Ù In-Fusion
®
Snap Assembly¿¡¼ ÈξÀ ¸¹Àº colony ¼ö¸¦ º¸¿´´Ù.
In-Fusion
®
Snap Assembly¿Í Ÿ»ç Á¦Ç° (NEBuilder HiFi)¸¦ ÀÌ¿ëÇØ Á¦ÇÑÈ¿¼Ò¸¦ ó¸®ÇÏ¿© Á¦ÀÛÇÑ 5’ overhang (A), blunt end (B), 3’ overhang (C)ÀÇ ´ÜÀÏ insert (3.8 kb)¸¦ ¼±ÇüÈÇÑ Ç¥ÁØ vector¿¡ cloningÇÏ¿´´Ù. Cloning ¹ÝÀÀÀº °¢°¢ ¼¼ ¹ø ¹Ýº¹ ½ÇÇèÇÏ¿´°í, °¢°¢ »ý¼ºµÈ colony¸¦ 20°³ ¼±ÅÃÇÏ¿© Sanger sequencingÀ¸·Î ¼¿ÀÌ Á¤È®ÇÏ°Ô »ðÀԵǾú´ÂÁö È®ÀÎÇÏ¿´´Ù.
Applications
±×¸² 5. In-Fusion
®
Snap Assembly Master MixÀÇ ´Ù¾çÇÑ applications ¿¹½Ã
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
°ü·ÃÁ¦Ç°
- ÆíÀǼºÀÌ ÁÁÀº ½Ç¿Â º¸°ü master mix,
In-Fusion
®
Snap Assembly EcoDry™ Master Mix (Code 638954)
- In-Fusion
®
°ú ¦²á, ÃÖ°íÀÇ Á¤È®µµ¸¦ Áö´Ñ PCRÈ¿¼Ò,
PrimeSTAR
®
Max DNA Polymerase (Code R045A)
- In-Fusion
®
Cloning¿¡ ÃÖÀûÈµÈ °íÈ¿À² competent cells,
Stellar™ Competent Cells (Code 636763)
- Touch down PCR ¹× gradient PCR ¼³Á¤ÀÌ °¡´ÉÇÑ °£ÆíÇÏ°í ÄÄÆÑÆ®ÇÑ PCR ±â±â,
Clontech PCR Thermal Cycler GP (Code WN400)
- Plasmid DNA¸¦ ½Å¼ÓÇÏ°Ô Á¤Á¦ÇÒ ¼ö ÀÖ´Â,
TaKaRa MiniBEST Plasmid Purification Kit (Code 9760A ¿Ü)
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Keyword : infusion,in-fusion,cloning,PCR cloning,cloning,Ŭ·Î´×,º¹¼ö´ÜÆí,ÀÎÇ»Àü,ÇÁ·Î¹Í½º,premix, mutagenesis,mutation,soeasy,so easy,639648,639649,639650,636763,638909,638910,638911
In-Fusion Cloning
[Overview] In-Fusion¢ç Cloning
[°³¿ä] So Easy Product
Online Tools for In-Fusion
ÃÖ°íÀÇ Colony Çü¼º´É°ú Á¤È®ÇÑ PCR cloning
[NEW] In-Fusion¢ç Snap Assembly
½Ç¿Â º¸°ü Master mix
[NEW] µ¿°á°ÇÁ¶ In-Fusion¢ç Snap Assembly
PremixÈ! ½Ã°£´ÜÃà! È¿À²¼º Çâ»ó!
[Kit] In-Fusion¢ç Cloning
In-Fusion¢ç cloningÀ» ¹Ù·Î ÁøÇàÇϱâ À§ÇÑ lin..
In-Fusion¢ç Ready Vectors