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Home > ÀüÁ¦Ç°º¸±â > PCR > PCR Enzyme (Clontech) > Advantage 2 Polymerase Mix & PCR Kit
ClontechÀÇ µ¶ÀÚÀûÀÎ blend Çü È¿¼Ò

Advantage 2 Polymerase Mix & PCR Kit

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(ºÎ°¡¼¼º°µµ)
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Clontech
639201
Advantage 2 Polymerase Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ȸ
588,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32401, x32402
Clontech
639202
Advantage 2 Polymerase Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ȸ
2,345,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639206
Advantage 2 PCR Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ȸ
1,015,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32401, x32402
Clontech
639207
Advantage 2 PCR Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
30 ȸ
330,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639137
10¡¿ Advantage 2 PCR Buffer
°ü·ÃÇмú ±¸¸ÅÇϱâ
600 ¥ìl ¡¿ 2
180,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639138
10¡¿ Advantage 2 PCR Buffer
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ml
396,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

Á¦Ç°¼³¸í
*º» ¸Þ´º¾óÀº 2009³â 12¿ù ¾÷µ¥ÀÌÆ® µÇ¾ú½À´Ï´Ù. - ±ä fragment ÁõÆø°ú Á¤È®¼ºÀ» ¿äÇÏ´Â PCR¿¡ ÀÌ»óÀû
- ³ôÀº °¨µµ¿Í ÁõÆø È¿À²
Advantage 2 polymerase mix´Â template size°¡ ±æ°Å³ª, º¸´Ù ³ôÀº fidelity¿Í ÁõÆø È¿À²À» ÇÊ¿ä·ÎÇÏ´Â ½ÇÇèÀ» ¸ñÀûÀ¸·Î ÃÖÀûÈ­µÈ PCR È¿¼Ò mixÀÌ´Ù. Advantage 2´Â ÀϹÝÀûÀÎ PCR È¿¼Òº¸´Ù fildelity°¡ 3¹è°¡ ³ô°í º¸´Ù À¯¿¬ÇÏ¸ç °á°ú°¡ ÀÏÁ¤ÇÑ long-distance PCR ½ÇÇèÀ» ÇÒ ¼ö ÀÖ´Ù. Advantage 2´Â Æø³ÐÀº ¹üÀ§¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Â polymerase mix·Î, ±¤¹üÀ§ÇÑ DNA ÁÖÇüÀ» °£´ÜÈ÷ ÁõÆøÇÒ ¼ö ÀÖ´Ù. ±âÁ¸¿¡´Â Advantage genomic PCR enzyme systemÀÇ »ç¿ëÀÌ ÇÊ¿äÇß´ø ´Ù¾çÇÑ ÁÖÇü¿¡¼­µµ ±× ´ëºÎºÐÀº Advantage 2·Î ÁõÆøÀÌ °¡´ÉÇÏ´Ù. Advantage 2 polymerase mix¿¡´Â ¾Æ·¡ÀÇ ¼ººÐÀÌ Æ÷ÇԵǾî ÀÖ´Ù.
- TITANIUM Taq DNA polymerase : Taqº¸´Ùµµ ³ôÀº ÁõÆø·®°ú °¨µµ¸¦ Áö´Ï¸ç Taq Antibody¸¦ »ç¿ëÇÏ´Â hot start PCR
- Proofreading polymerase : PCR »ê¹°ÀÇ size¿Í fildelity Çâ»óÀ» ¸ñÀûÀ¸·Î ¼Ò·®ÀÇ Proofreading polymerase°¡ Æ÷ÇԵǾî ÀÖ´Ù.
Advantage 2 polymerase mix´Â SMART RACE cDNA amplifcation Kit, Super SMART PCR cDNA synthesis Kit, Clontech PCR-select subtraction Kit(193 page) µîÀÇ Kit¿Í ÇÔ²² »ç¿ëÇϱ⿡µµ ÀûÇÕÇÏ´Ù.

Advantage 2 polymerase mix Á¦Ç°Àº ÃÖÀûÈ­µÈ buffer°¡ Æ÷ÇԵǾî ÀÖ´Â ¿ÏÀüÇÑ PCR KitÀÌ ÁغñµÇ¾î ÀÖ´Ù.

±×¸² 1. Advantage 2 polymerase mix ¹× Ÿ»ç Taq polymerase mix¿¡ ÀÇÇÑ TNFR¥± cDNAÀÇ ÁõÆø
°¢ polymerase¿¡¼­ ¾òÀº PCR»ê¹° 5 §¡¸¦ »ç¿ëÇÏ¿© 1.1 % agarose/EtBr gel·Î Àü±â¿µµ¿ ÇÏ¿´´Ù. Lane 1 : Advantage 2. Lane 2 : Ÿ»ç Taq polymerase. Lane M : DNA size marker. Advantage 2¿¡¼­¸¸ 0.4 kb TNFR¥± ÁõÆøÀÌ È®ÀεǾú´Ù.


±×¸² 2. Advantage 2 polymerase mix¸¦ ÀÌ¿ëÇÑ ´Ù¾çÇÑ ±æÀÌÀÇ ÁõÆø
¾Æ·¡¿Í °°Àº Á¶°Ç¿¡¼­ ¾òÀº PCR »ý¼º¹° 1~3 §¡¸¦ »ç¿ëÇÏ¿© 1.1 % agarose/EtBr gel·Î Àü±â¿µµ¿À» ÇÏ¿´´Ù. Lane 1 : 2.5 kbÀÇ E.coli DNA polymerase À¯ÀüÀÚ¸¦ genome DNA¿¡¼­ ÁõÆø. Lane 2 : 3.5 kbÀÇ bovine pancreatic trypsin inhibitor À¯ÀüÀÚ¸¦ calf thymus genomic DNA¿¡¼­ ÁõÆø. Lane 3 : 5.9 kbÀÇ human IL-1¥â À¯ÀüÀÚ¸¦ »ç¶÷ genome DNA¿¡¼­ ÁõÆø. Lane 4 : 8.5 kbÀÇ human titin cDNA¸¦ SMART Human Skeletal Muscle cDNA library¿¡¼­ ÁõÆø. Lane 5 : 18.5 kbÀÇ ¥ë insert¸¦ ¥ë clone¿¡¼­ ÁõÆø. Lane M : ¥ë/Hind¥² DNA size marker.
±¸¼º¹°
Advantage 2 polymerase mix ±¸¼º¹° - Advantage 2 polymerase mix - Advantage 2 PCR buffer - User manual(PT3281-1) - Protocol-at-a-glance(PT3281-2) Advantage 2 PCR Kit ±¸¼º¹° - Advantage 2 polymerase mix - Advantage 2 PCR buffer - dNTP(10 mM each) - Control DNA template - Control primer mix - PCR-grade water - User manual(PT3281-1) - Protocol-at-a-glance(PT3281-2)
º¸°ü -20 ¡É
Àû¿ëºÐ¾ß
- Long and accurate PCR - Preparative PCR - RACE - cDNA synthe

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