´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Cloned

Terminal Deoxynucleotidyl Transferase

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2230A
Terminal Deoxynucleotidyl Transferase
°ü·ÃÇмú ±¸¸ÅÇϱâ
300 U
64,800¿ø 
81,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
2230A_DS.v2402Da.pdf
Takara
2230B
Terminal Deoxynucleotidyl Transferase
°ü·ÃÇмú ±¸¸ÅÇϱâ
1,500 U (2230A x 5)
365,000¿ø 

Á¦Ç°¼³¸í
º» È¿¼Ò´Â ÁÖÇü ¾øÀÌ ´ÜÀÏ °¡´Ú ¶Ç´Â ÀÌÁß °¡´ÚÀÇ DNA 3¡¯-OH¸»´Ü¿¡ deoxynucleotide¸¦ ÁßÇÕÇÏ´Â ¹ÝÀÀÀ» Ã˸ŠÇÑ´Ù. À̶§ primer·Î ÃÖ¼Ò 3¿°±â ÀÌ»óÀÇ deoxynucleotide¸¦ ÇÊ¿ä·Î ÇÑ´Ù.
TdT¸¦ ÀÌ¿ëÇÑ ´ÜÀÏ ÁßÇÕüÀÇ ºÎ°¡ ¹ÝÀÀÀÇ Á¶°ÇÀº
   1. ºÎ°¡ÇÏ´Â ¿°±âÀÇ Á¾·ù (dATP, dTTP, dCTP, dGTP)
   2. ¹öÆÛ¿¡ ÀÖ´Â 2°¡ ÀÌ¿ÂÀÇ Á¾·ù (Mn2+, Co2+, Mg2+)
   3. ºÎ°¡µÇ´Â DNAÀÇ ¸»´Ü±¸Á¶ (µ¹Ãâ 3¡¯-OH ¸»´Ü, ÆòÈ° ¸»´Ü, ÇÔ¸ô 3¡¯-OH ¸»´Ü)
µî¿¡ ¿µÇâÀ» ¹Þ±â ¶§¹®¿¡ »óȲ¿¡ ¸Â´Â ÃÖÀûÁ¶°ÇÀ» °ËÅäÇÒ °ÍÀ» ÃßõÇÑ´Ù.
º¸Á¸
-20¡É
±â¿ø
E. coli carrying the plasmid which encodes the gene of terminal deoxynucleotidyl transferase
³óµµ
14 U/§¡
Çü»ó

60 mM

Potassium Phosphate ¿ÏÃæ¿ë¾× (pH7.2)

150 mM

KCl

1 mM

DTT

50%

Glycerol

÷ºÎ buffer Á¶¼º (5x)
(Tailing ¹ÝÀÀ¿ë)
500 mM

HEPES (pH7.2)

40 mM

MgCl2

0.5 mM

DTT

0.1%

BSA [º°Ã·]

È°¼ºÀÇ Á¤ÀÇ
37¡É, pH7.2¿¡¼­ DNase ó¸® ÈÄ ¿­ º¯¼º ¼Û¾ÆÁö Èä¼± DNA (È°¼ºÈ­ ¼Û¾ÆÁö Èä¼± DNA)¸¦ initiator·Î 1½Ã°£ µ¿¾È 1 nmolÀÇ [3H]dATP¸¦ »ê¼ºÀÇ Ä§Àü¹°À»(acid-insoluble products) ÇÕ¼ºÇÏ´Â È¿¼ÒÈ°¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
È°¼º ÃøÁ¤¿ë ¹ÝÀÀ¾× Á¶¼º

100 mM

Sodium cacodylate ¿ÏÃæ¿ë¾× (pH7.2)

8 mM

MgCl2

0.1 mM

DTT

0.024%

BSA

160 §¶/§¢

È°¼ºÈ­ ¼Û¾ÆÁö Èä¼± DNA

0.5 mM

[3H]dATP

»ç¿ë»óÀÇ ÁÖÀÇ
TdT¸¦ ÀÌ¿ëÇÑ ´ÜÀÏ ÁßÇÕüÀÇ ºÎ°¡ ¹ÝÀÀÀÇ Á¶°ÇÀº ºÎ°¡ÇÏ´Â ¿°±âÀÇ Á¾·ù (dATP, dTTP, dCTP, dGTP), buffer ¾ÈÀÇ 2°¡ ÀÌ¿ÂÀÇ Á¾·ù (Mn2+, Co2+, Mg2+) ¹× ºÎ°¡µÇ´Â DNAÀÇ ¸»´Ü±¸Á¶ (3¡¯-OH µ¹Ãâ ¸»´Ü, ÆòÈ° ¸»´Ü, 3¡¯-OH ÇÔ¸ô ¸»´Ü)µî¿¡ ¿µÇâÀ» ¹Þ±â ¶§¹®¿¡ »óȲ¿¡ ¸Â´Â ÃÖÀû Á¶°ÇÀ» ã¾Æ¾ß ÇÑ´Ù. ÀϹÝÀûÀ¸·Î dATP, dTTP¸¦ ºÎ°¡ÇÏ´Â °æ¿ì Co2+À» Æ÷ÇÔÇÏ´Â buffer°¡, dCTP, dGTP¸¦ ºÎ°¡ÇÏ´Â °æ¿ì Mn2+¸¦ Æ÷ÇÔÇÑ buffer°¡ ÃÖÀûÀÌ´Ù. ¶ÇÇÑ DNAÀÇ 3¡¯-OH ¸»´Ü¿¡ 15~40 baseÀÇ deoxynucleotide¸¦ ºÎ°¡Çϱâ À§ÇÑ dNTP¿Í DNAÀÇ ÃÖÀû Á¶°ÇÀº 20 : 1ÀÇ ¸ô¼öÀÌ°í, 3¡¯-OH ÇÔ¸ô ¸»´ÜÀÇ °æ¿ì 100:1ÀÇ ¸ô¼öÀÌ´Ù.

CoCl2 ¹öÆÛ
100 mM

Sodium cacodylate (pH7.2)

1 mM

CoCl2

0.1 mM

DTT


MnCl2 ¹öÆÛ
100 mM

Sodium cacodylate (pH7.2)

2 mM

MnCl2

0.1 mM

DTT

¿ëµµ
- dNTP Ç¥½Ä ȤÀº ddNTP¿¡ ÀÇÇÑ DNAÀÇ 3¡¯-¸»´Ü Ç¥½Ä
- Okayama-Berg¹ý¿¡ ÀÇÇÑ º¤ÅÍ cDNA¿¡ »óº¸ÀûÀÎ ´ÜÀÏ ÁßÇÕü ºÎ°¡
ÀϹÝÀû ¼ºÁú
- ºÐÀÚ·®: ¾à 60,000 (À¯ÀüÀÚ¸¦ ÅëÇÑ ÃßÁ¤)
- ÃÖÀû pH: pH7.2
- º¸Á¶ÀÎÀÚ: Mg2+, Mn2+, Co2+ µîÀÇ 2°¡ ÀÌ¿Â Áß Çϳª¸¦ ÇÊ¿ä·Î ÇÑ´Ù.
- ÀúÇØÁ¦: ´Ù·®ÀÇ ¾çÀ̿ ȤÀº À½À̿¿¡ ÀúÇصȴÙ.
- ±âŸ
   Nucleotide analog (5-methyl-dCTP, 6-O-methyl-dGTP µî)µµ ±âÁú·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù.
   Deoxythymidine 3Àλê ȤÀº cordycepin 3ÀλêÀº Å͹̳×ÀÌÅÍ·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù.

Keyword :

¼ö½ÄÈ¿¼Ò£¯DNA Polymerase¡¢RNA Polymerase
>
dsRNA È¥ÀÔÀÌ Àû¾î ÀÓ»óºÐ¾ßÀÇ ¿¬±¸°³¹ß¿¡ ÀûÇÕ..
PrimeCap¢â T7 RNA Polymerase (low dsRNA)