´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
°£ÆíÇØÁø baculovirus ¿ª°¡ ÃøÁ¤ Å°Æ®

BacPAK¢â p35 ELISA Kit (Single Wash)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631477
BacPAK¢â p35 ELISA Kit (Single Wash)
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 ȸ
1,336,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x347928, x347926

  • BaculovirusÀÇ titer¸¦ ELISA·Î °£ÆíÇÏ°Ô ÃøÁ¤
  • °¨¿°À¸·ÎºÎÅÍ 20½Ã°£ À̳»¿¡ ¿ª°¡ È®ÀÎ °¡´É, ¾à 2½Ã°£ÀÇ hands on time
  • 1¡¿105 ~ 107 pfu/§¢ (IFU/§¢) ±¤¹üÀ§ÇÑ ¿ª°¡ ÃøÁ¤ °¡´É (°ËÃâÇÑ°è: 104 pfu/§¢)
Á¦Ç° ¼³¸í
BacPAK p35 ELISA Kit (Single Wash)´Â baculovirus¿¡¼­ ¹ßÇöÇÏ´Â p35ÀÇ ³óµµ (ng/§¢)¸¦ ELISA¹ýÀ¸·Î Á¤·®ÇÏ´Â Á¦Ç°À¸·Î, ÀÌ¹Ì ¿ª°¡¸¦ ¾Ë°í ÀÖ´Â reference virus stockÀÇ p35 °ª°ú ºñ±³/ȯ»ê*À» ÅëÇØ ¾Ë°íÀÚ ÇÏ´Â baculovirus »ùÇÃÀÇ functional titer¸¦ °áÁ¤ÇÒ ¼ö ÀÖ´Ù.
p35´Â ¹ÙÀÌ·¯½ºÀÇ ¼¼Æ÷ °¨¿°¿¡ ÇʼöÀûÀÎ Ãʱ⠹ßÇö ´Ü¹éÁú·Î °¨¿° ½Ã ¼¼Æ÷ÀÇ apoptosis¸¦ ÀúÇØÇÑ´Ù. °¨¿° 4~12½Ã°£ ÈÄ ¼¼Æ÷Áú ±âÁú¿¡¼­ °ËÃâµÇ¹Ç·Î, ¼¼Æ÷ÀÇ ÇüÅ º¯È­³ª ¹ÙÀÌ·¯½º Ãâ¾Æ°¡ ÀϾ±â Àü¿¡ ¹ÙÀÌ·¯½º ´Ü¹éÁúÀÇ ¹ßÇöÁ¤µµ¸¦ Á¤È®ÇÏ°Ô ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº ¾à 2½Ã°£ÀÇ hands on timeÀ¸·Î 20½Ã°£ À̳»¿¡ baculovirus ¿ª°¡¸¦ ÃøÁ¤Çϸç, 104 pfu/§¢ (IFU/§¢) ÀÌ»óÀÇ ¹ÙÀÌ·¯½ºÀÇ ÃøÁ¤ÀÌ °¡´ÉÇÏ´Ù.
*»ùÇÃÀÇ ¿ª°¡¸¦ ÃøÁ¤Çϱâ Àü¿¡, ¹Ì¸® ¿ª°¡¸¦ ¾Ë°í ÀÖ´Â reference virus¸¦ Å×½ºÆ®ÇÏ¿© p35 °ªÀ» ¾òÀ¸¸é, ¿ª°¡ È®ÀÎÀÌ ÇÊ¿äÇß´ø »ùÇÃÀÇ pfu/§¢ (IFU/§¢) ´ÜÀ§ ¿ª°¡·Îµµ °è»ê(ȯ»ê)ÇÒ ¼ö ÀÖ´Ù.
- ½±°Ô ȯ»êÇÏ´Â ¿Â¶óÀÎ tool ¹Ù·Î°¡±â: Baculovirus p35-to-IFU calculator (TB USA ÆäÀÌÁö·Î À̵¿ÇÕ´Ï´Ù.)



±×¸² 1. BacPAK¢â p35 ELISA Kit (Single Wash) workflow
A. ¹ÙÀÌ·¯½º »ùÇà serial dilution
B. Èñ¼®ÇÑ ¹ÙÀÌ·¯½º¸¦ °ïÃæ ¼¼Æ÷¿¡ °¨¿°½ÃŲ ÈÄ 27¡É, 16~20½Ã°£ incubation
C. ÷ºÎµÈ Lysis Buffer·Î °¨¿° ¼¼Æ÷¸¦ ¿ëÇØÇÏ°í ELISA·Î »ùÇÃÀÇ ¿ª°¡ (p35°ª)¸¦ ÃøÁ¤ (ng/§¢)



±×¸² 2. BacPAK¢â p35 ELISA Kit (Single Wash) ¿ø¸®
ELISA plate¿¡ cell lysate¿Í HRP-anti-p35 detection antibody¸¦ Ãß°¡Çϸé, »ùÇà ³»ÀÇ p35´Â Ç÷¹ÀÌÆ®¿¡ °íÁ¤È­µÇ¾î ÀÖ´Â capture antiboody¿Í °áÇÕÇÏ°í, HRP-anti-p35 detection antibody¿Í °áÇÕÇÏ¿© Ç¥ÁöµÈ´Ù. Washing Buffer·Î ¼¼Ã´ ÈÄ, TMB Substrate·Î ¹ß»ö½ÃŲ´Ù. Stop SolutionÀ¸·Î ¹ÝÀÀÀ» Á¤Áö½ÃŲ ÈÄ, Èí±¤µµ (450 nm)¸¦ ÃøÁ¤ÇÏ¿© p35 ³óµµ (ng/§¢)¸¦ Á¤·®ÇÑ´Ù.
³»¿ë
  • Anti-p35 Coated Plate (96 well£º8 well¡¿12 strips)
  • p35 Control
  • Lysis Buffer
  • p35 Buffer
  • Anti-p35 (HRP Conjugate)
  • Washing Buffer (20¡¿)
  • TMB Substrate
  • Stop Solution
º¸Á¸ 4¡É
Washing Buffer (20¡¿)¸¦ ³ÃÀå º¸°üÇϸé ħÀüÀÌ ¹ß»ýÇÒ °¡´É¼ºÀÌ ÀÖ´Ù.
- ħÀüÀÌ ³ªÅ¸³­ °æ¿ì 37¡É¿¡¼­ ¿ëÇØÇÏ¿© »ç¿ë
°ü·ÃÁ¦Ç°
- Baculovirus ½Ã½ºÅÛ ¼Â¾÷À» À§ÇÑ all-in-one kit: BacPAK¢â Baculovirus Expression System
- Insect cell line: IPLB-Sf21 Insect Cells
- Insect cell Àü¿ë transfection ½Ã¾à: TransIT¢ç-Insect Transfection Reagent
- ´Ù¾çÇÑ ¿ª°¡ÃøÁ¤ kit
   qPCR·Î ºü¸£°Ô genome copy ÃøÁ¤: BacPAK¢â qPCR Titration Kit
   ¸é¿ªÇÐÀû ºÐ¼®¹ýÀ¸·Î ±î´Ù·Î¿î plaque ºÐ¼® ºÒÇÊ¿ä: BacPAK¢â Baculovirus Rapid Titer Kit
- ÇÕ¸®ÀûÀÎ °¡°Ý, °¡¼ººñ ³ôÀº qPCR ±â±â CronoSTAR¢â 96 Real-Time PCR System(4ch)

Keyword :

°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Baculovirus)
>
10ºÐ ¿Ï·á - baculovirus ½Å¼Ó¿ª°¡ ÃøÁ¤
BacPAK¢â Baculovirus GoStix¢â Plus
>
°£ÆíÇØÁø baculovirus ¿ª°¡ ÃøÁ¤ Å°Æ®
BacPAK¢â p35 ELISA Kit (Single Wash)
>
Positive / negative control ¿ë baculovirus
Baculovirus control virus / transfer vector
>
Baculovirus DNA & transfer vector set
Baculovirus DNA package kit
>
¸é¿ªÇÐÀû ÃøÁ¤¹ýÀ¸·Î 48 ½Ã°£À¸·Î Baculovirus ..
Baculovirus Titer Kit
>
°ïÃæ, Æ÷À¯·ù ¼¼Æ÷¿ë ÀçÁ¶ÇÕ ´Ü¹éÁú °í¹ßÇö ½Ã..
flashBAC¢â Baculovirus Expression System
>
ºÐÀÚ ChaperoneÀ» ¹ßÇöÇÏ´Â Recombinant Baculo..
FoldHelper Helper virus
>
Plaque Assay ¼øÈ­°¡ ºÒÇÊ¿äÇÑ È¿À²ÀûÀÎ Recomb..
ProEasy Baculovirus Vectors
>
Sf9, Sf21 cell¿¡ ÃÖÀûÈ­µÈ Transfection reage..
ProFectin
>
Chaperone Æ÷ÇÔ - ´Ü¹éÁú °¡¿ëÈ­ È¿À² Çâ»ó
ProFold Baculovirus Vectors
>
Baculovirus ¿ª°¡£¨Genomic DNA copy ¼ö£©¸¦ ..
qPCR Titer ÃøÁ¤ Kit
>
¸ñÀû¿¡ µû¶ó ¼±ÅÃÇÏ¿© Baculovirus Á¦ÀÛ
Transfer Vectors