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ÃÑ 15ºÐÀ̸é Immunoprecipitation (IP, Co-IP) ½ÇÇè ¿Ï·á

Capturem¢â IP £¦ Co-IP Kit

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Clontech
635721
Capturem¢â IP & Co-IP Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
12 ȸ
(Package)
440,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32542

  • Complete Kit - Immunoprecipiation(IP), Co-Immunoprecipitation(Co-IP) ½ÇÇè¿¡ ÃÖÀûÈ­µÈ Column°ú Buffer°¡ Æ÷ÇÔ
  • Simple & fast workflow - Sample°ú IP¿ë Ç×ü¸¦ 10 min incubation ÈÄ, Capturem¢â columnÀ» ÀÌ¿ëÇØ ½Ç¿Â¿¡¼­ 5ºÐ¸¸¿¡ Á¤Á¦
  • High concentration - ¾ÆÁÖ ÀûÀº elution volumeÀ» ÀÌ¿ëÇϹǷΠ°í³óµµÀÇ IP Complex Á¤Á¦ °¡´É
  • High quality - ½Å¼ÓÇÑ 5ºÐ ÇÁ·ÎÅäÄÝ·Î ´Ü¹éÁú aggregation, complex dissociation ¶Ç´Â ½ÇÈ° (loss of activity)¸¦ ¹æÁö
  • Versatile - ´Ù¾çÇÑ Á¶°Ç°ú ´Ù¾çÇÑ IP Buffer¿¡ Àû¿ë °¡´É
  • Single-use - Novel disposable columnÀÇ »ç¿ëÀ¸·Î »ùÇÃÀÇ contamination, carryover ¹æÁö
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Rapid Protein A-based Immunoprecipitation
Immunoprecipiation(IP)°ú Co-immunoprecipiation(Co-IP)´Â ´Ü¹éÁú°ú ´Ù¸¥ ´Ü¹éÁú ¶Ç´Â °Å´ëºÐÀÚ (Macromolecules) °£ÀÇ »óÈ£ÀÛ¿ëÀ» ¿¬±¸ÇÏ´Â µ¥ À¯¿ëÇÑ ½ÇÇè ¹æ¹ýÀÌ´Ù. Capturem¢â IP & Co-IP Kit´Â ´Ü¹éÁú ¶Ç´Â ´Ü¹éÁú-´Ü¹éÁú(Ç×ü) º¹ÇÕü¸¦ ºü¸£°í °£ÆíÇÏ°Ô Á¤Á¦ÇÒ ¼ö ÀÖ´Â ¿ëÀÌÇÑ Á¦Ç°ÀÌ´Ù. º» Á¦Ç°Àº Ç×ü¿Í °áÇÕµÈ ´Ü¹éÁúÀ» Á¤Á¦ÇÒ ¼ö ÀÖ´Â Capturem¢â Protein A columnsÀ» ÀÌ¿ëÇϸç Cell lysis, binding, washing ¹× Ç×ü-Ç׿ø º¹ÇÕü ¿ëÃâÀ» À§ÇÑ Elution buffer±îÁö ¸ðµÎ Á¦Ç° ³»¿¡ Æ÷ÇԵǾîÀÖ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇÏ¸é ½Ç¿Â¿¡¼­ 15ºÐ¸¸¿¡ ImmunoprecipitationÀ» ¿Ï·áÇÒ ¼ö ÀÖ´Ù.


±×¸² 1. Capturem Protein A technology.
Each Capturem Protein A spin column contains a high-capacity Protein A membrane with increased surface area, providing much higher antibody binding capabilities per ml of membrane than per ml of resin.


±×¸² 2. Comparison of Capturem IP & Co-IP Kit workflow to that of a leading competitor.


±×¸² 3. Capturem IP & Co-IP Kit workflow.
Each mini spin column can be loaded with up to 800 ¥ìl of sample containing the antibody-antigen complex. Antibodies are first bound to equilibrated membrane, followed by washing with 100 ¥ìl of wash buffer, and elution with 30/50 ¥ìl of elution buffer. Each step is followed by spinning the tube for 1 min at 1,000g. This entire purification is complete in ~15 min.
Applications
  • Immunoprecipitation (IP)
  • Co-immunoprecipitation (Co-IP)
  • Investigation of protein complexes
  • Antigen screening for antibody discovery and engineering workflow
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)

Capturem¢â IP&Co-IP Columns

12°³

Lysis/Equilibration Buffer

12 ml

Wash Buffer

2 ml

Elution Buffer

1 ml

Protease Inhibitor Cocktail(100¡¿)

100¥ì l

º¸Á¸

Capturem¢â IP&Co-IP Columns

½Ç¿Â

±âŸ

4¡É


Keyword : Protein A,Ç×ü,Ç×üÁ¤Á¦,Antibody,Capturem,IP,Immunoprecipiation,¸é¿ªÄ§°­

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ÃÑ 15ºÐÀ̸é Immunoprecipitation (IP, Co-IP) ..
Capturem¢â IP £¦ Co-IP Kit