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Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö Vector

pBApo-EF1¥á Vector ½Ã¸®Áî

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
3243
pBApo-EF1¥á Neo DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
423,000¿ø  3243_3244_e.v1612Da.pdf
Takara
3244
pBApo-EF1¥á Pur DNA
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
423,000¿ø  3243_3244_e.v1612Da.pdf

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pBApo-EF1¥á´Â Æ÷À¯·ù ¼¼Æ÷¿ë À¯ÀüÀÚ expression vector·Î Human elongation factor-1 ¥á À¯ÀüÀÚ (EF1 ¥á promoter) ¹× Herpes Simple Virus Thymidine KinaseÀÇ polyA signalÀ» žÀçÇÏ°í ÀÖ´Ù. Cloning site¿¡ ¸ñÀû À¯ÀüÀÚÀÇORF¸¦ »ðÀÔÇÏ¿© ¸ñÀû À¯ÀüÀÚÀÇ expression plasmid¸¦ ¾òÀ» ¼ö ÀÖÀ¸¸ç, À¯ÀüÀÚ ¹ßÇö ÀÌ¿Ü¿¡ pri-microRNA µîÀÇ Àü»ç »ê¹° ¹ßÇö¿¡µµ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ ½±°Ô ¡¸promoter£«ORF£«polyA signal¡¹ cassette¸¦ adenovirus vector·Î ¿Å±æ ¼ö ÀÖÀ¸¸ç, À̶§ ÀçÁ¶ÇÕ adenovirus Á¦ÀÛ¿¡´Â Adenovirus Expression Vector Kit (Dual Version) Ver. 2 (Code 6170)¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
Adenovirus´Â ³ôÀº °¨¿°È¿À²°ú ³ÐÀº °¨¿°´É·ÂÀ» °¡Áö°í ÀÖ¾î in vitro ¶Ç´Âin vivo À¯ÀüÀÚ µµÀÔ¿¡ ÀûÀýÇÏ´Ù. pBApo-EF1¥á½Ã¸®Áî¿¡¼­´Â mammalian cellÀÇ selection marker·Î neomycin ³»¼º À¯ÀüÀÚ ¶Ç´Â puromycin ³»¼º À¯ÀüÀÚ¸¦ žÀçÇÑ vector°¡ ÀÖ¾î, ½ÇÇè ¸ñÀû¿¡ µû¶ó ÀûÀýÇÑ vector¸¦ ¼±ÅÃÇÒ ¼ö ÀÖ´Ù.
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pBApo-EF1¥á Neo DNA (500 ng/¥ìl) 20 ¥ìg
¶Ç´Â pBApo-EF1¥á Pur DNA (500 ng/¥ìl)
º¸Á¸ -20¡É
Çü»ó
10 mM Tris - HCl, pH8.0
1 mM EDTA
pBApo-EF1¥á Neo DNA, pBApo-EF1¥á Pur DNAÀÇ ±¸Á¶

pBApo - EF1¥á Neo , pBApo - EF1¥á Pur ÀÇ Å¬·Î´× ±×¸²

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