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cDNA ÇÕ¼º
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Reverse Transcription (Takara)
> Recombinant RNase Inhibitor
RT-PCR ÇʼöÇ°
Recombinant RNase Inhibitor
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Takara
2313A
Recombinant RNase Inhibitor
5,000 U
131,000¿ø
Takara
2313B
Recombinant RNase Inhibitor
25,000 U (2313A x 5)
595,000¿ø
Product index
Reverse Transcription (Tak..
High fidelity RTase, P..
High Fidelity, Full Le..
PrimeScript¢â IV 1st s..
PrimeScript¢â II 1st s..
PrimeScript¢â Reverse ..
PrimeScript¢â 1st stra..
Reverse Transcriptase ..
Recombinant RNase Inhi..
Reverse Transcriptase ..
Ribonuclease Inhibitor
cDNA Library Construct..
¿ªÀü»ç ¹ÝÀÀ¿ë Primer (..
Double strand cDNA ÇÕ..
Oligo(dT) 15 primer
Reverse Transcription (Clo..
[°³¿ä] So Easy Product
µ¿°á°ÇÁ¶Å¸ÀÔ RT Premix
MMLV Reverse Transcrip..
SMARTScribe Reverse Tr..
Advantage RT-for-PCR K..
SMART MMLV Reverse Tra..
TITANIUM One-Step RT-P..
SMART¢ç Full length cDNA ..
[°³¿ä] SMARTer¢ç cDNA ..
RACE 5/3 Kit (Unknown ..
Full length cDNA Synth..
Marathon cDNA Amplific..
cDNA Library (Human, Mouse..
Human cDNA Library
Rat cDNA Library
Premade cDNA
Á¶Á÷ ¹× ¼¼Æ÷ÁÖ À¯·¡ÀÇ ..
QUICK-Clone cDNA (Huma..
QUICK-Clone cDNA (Mous..
QUICK-Clone cDNA (Rat)
RACE ¿ë Marathon-Ready..
RACE ¿ë Marathon-Ready..
RACE ¿ë Marathon-Ready..
Total RNA£¦Poly A RNA
Human Tumor, Human Cel..
Premium Poly A+ RNA (H..
Premium Poly A+ RNA (H..
Premium Total RNA (Hum..
Premium Total RNA (Mou..
Premium Total RNA (Rat..
qPCR Human Reference c..
Total RNA Master Panel
Universal Reference To..
±× ¿Ü °ü·ÃÁ¦Ç° (RNA)
RNase-free Water
RNase-OFF¢ç
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Recombinant RNase Inhibitor´Â µÅÁö °£ À¯·¡ÀÇ RNase inhibitor¸¦ ´ëÀå±Õ ¼÷ÁÖ¿¡¼ ¹ßÇö, affinity column µîÀ» ÀÌ¿ëÇÏ¿© Á¤Á¦ÇÑ ÀçÁ¶ÇÕ ´Ü¹éÁúÀÌ´Ù. ÀÌ´Â Àΰ£ Å¹ÝÀ̳ª µÅÁö °£¿¡¼ Á÷Á¢ Á¤Á¦ÇÑ RNase inhibitor¿Í ¸Å¿ì À¯»çÇÑ ¼ºÁúÀ» °¡Áö¸ç, RNase A¿Í 1:1 º¹ÇÕü¸¦ Çü¼ºÇÏ¿© RNaseÀÇ ÀÛ¿ëÀ» ¾ïÁ¦ÇÑ´Ù.
ÀÌ·¯ÇÑ ¹ÝÀÀÀº °¡¿ªÀûÀ̹ǷÎ, urea ¶Ç´Â sulfhydryl ½Ã¾àÀ¸·Î º¹ÇÕü¸¦ Çظ® ½ÃÅ´À¸·Î½á RNaseÀÇ È°¼ºÀ» µÇ»ì¸®°í, inhibitor¸¦ ºñ°¡¿ªÀûÀ¸·Î ½ÇÈ° ½Ãų ¼ö ÀÖ´Ù. ¶ÇÇÑ, ±âÁ¸ÀÇ ±æÇ×¼º ÀúÇØÁ¦ (nucleotide·ù, ¹«±âÀλê·ù)¿Í´Â ´Þ¸® ´Ü¹éÁúÀ̹ǷΠÆä³î 󸮷Π¹ÝÀÀ°è¿¡¼ °£´ÜÈ÷ Á¦°ÅÇÒ ¼ö ÀÖ´Ù. ¹Ý¸é RNase HÀÇ È°¼ºÀº ÀúÇØÇÏÁö ¾Ê´Â´Ù.
ÀÌ Á¦Ç°Àº Àΰ£ Å¹ݿ¡¼ À¯·¡ÇÑ RNase¿Í µ¿ÀÏÇÑ ¿ëµµ·Î »ç¿ë °¡´ÉÇϸç human DNA ¿À¿°ÀÌ ¿ì·ÁµÇ´Â °æ¿ì À¯¿ëÇÏ°Ô »ç¿ëÇÒ ¼ö ÀÖ´Ù
º¸Á¸ -20¡É
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Çü»ó
20 mM
HEPES-KOH (pH 7.5)
50 mM
KCl
5 mM
DTT
50 %
Glycerol
È°¼ºÀÇ Á¤ÀÇ
5 ngÀÇ RNase A È°¼ºÀ» 50% ÀúÇØÇÏ´Â È¿¼Ò È°¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
(ÀúÇØ È°¼ºÀº RNase A¿¡ ÀÇÇÑ Cyclic 2’, 3’-CMPÀÇ °¡¼öºÐÇظ¦ ¾ïÁ¦ÇÏ´Â ´É·Â¿¡ ÀÇÇØ Á¤·® µÇ¾ú´Ù.)
¿ëµµ
- cDNA ÇÕ¼º ½Ã (RNase inhibitor, 0.5 U/§¡ reaction)
-
in vitro
translation (RNase inhibitor, 1 U/§¡ reaction)
-
in vitro
transcription with cell-free extract (RNase inhibitor, 20 U/§¡ reaction)
-
in vitro
transcription with SP6 or T7 polymerase (RNase inhibitor, 1 U/§¡ reaction)
- Polysome isolation (RNase Inhibitor, 1,000 U/§¢ reaction)
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- µÅÁö °£ À¯·¡ÀÇ RNase inhibitor À¯ÀüÀÚ¸¦ °¡Áø plasmid¸¦ ¹ßÇöÇÏ´Â
E. coli
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- ºÐÀÚ·®: ¾à 52,000
- µîÀüÁ¡: pH5.0
- ÃÖÀû pH: pH6~9
- ÃÖÀû ¿Âµµ: 25¡É ~ 50¡É
- È°¼ºÈÁ¦:
ȯ¿øÁ¦ DTT (free »óÅÂÀÇ -SH±â¸¦ º¸È£Çϱâ À§Çؼ storage buffer¿¡ ÃÖ¼Ò 1mMÀÇ DTT°¡ ÇÊ¿äÇÏ´Ù.)
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65¡É 15ºÐÀÇ ¿Ã³¸®·Î ºñÈ°¼ºÈ ÇÒ ¼ö ÀÖ´Ù.
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- ³ÐÀº pH ¹üÀ§¿¡¼ ¾ïÁ¦ È°¼ºÀ» º¸À̸ç pH7~8¿¡¼ ÃÖ°íÈ°¼ºÀ» º¸ÀδÙ.
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PrimeScript™ Reverse Transcriptase (Code 2680A)
- cDNA ÇÕ¼ºÀ» À§ÇÑ all-in-one ÃÖ½ÅÁ¦Ç°,
PrimeScript™ IV 1st strand cDNA Synthesis Mix (Code 6215A)
- °íÇ°Áú °í¼öÀ²
in vitro
transcriptionÀ» À§ÇÑ ½Ã½ºÅÛ,
Takara IVTpro™ mRNA Synthesis System (Code 6141)
- gDNA Eraser ColumnÀ¸·Î gDNA ¿À¿° ¾øÀÌ ´Ù¾çÇÑ »ùÇÃÀÇ RNA Á¤Á¦,
TaKaRa MiniBEST Universal RNA Extraction Kit (Code 9767A)
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Keyword :
Reverse Transcription (Takara)
High fidelity RTase, PrimeScript¢â
High Fidelity, Full Length cDNA ÇÕ¼º °ü·ÃÁ¦Ç°
Premix ŸÀÔÀÇ cDNA ÇÕ¼º ½Ã¾à
PrimeScript¢â IV 1st strand cDNA Synthesis Mix
¾Ç¼¼»ç¸® ´Ü¹éÁúÀÌ Ãß°¡ µÈ ÃÖ»ó±Þ Full-length..
PrimeScript¢â II 1st strand cDNA Synthesis Kit
Ź¿ùÇÑ ½ÅÀå´É·ÂÀ» °¡Áø ½Å°³³ä ¿ªÀü»ç È¿¼ÒÀÇ ..
PrimeScript¢â Reverse Transcriptase
PrimeScript RTase¸¦ ÀÌ¿ëÇÑ 1st strand cDNA ..
PrimeScript¢â 1st strand cDNA Synthesis Kit
Reverse Transcriptase XL (AMV)
RT-PCR ÇʼöÇ°
Recombinant RNase Inhibitor
RNA PCR KitÀÇ Component
Reverse Transcriptase XL (AMV) for RT-PCR Kit
Ribonuclease Inhibitor
cDNA Library ±¸Ãà All-in-One Kit
cDNA Library Construction Kit
¿ªÀü»ç ¹ÝÀÀ¿ë Primer (Random Primer)
Double strand cDNA ÇÕ¼º
¿ªÀü»ç¹ÝÀÀ¿ë primer
Oligo(dT) 15 primer