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TaKaRa
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RT-PCR ÇʼöÇ°

Recombinant RNase Inhibitor

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
2313A
Recombinant RNase Inhibitor
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
5,000 U
131,000¿ø  2313A_DS.v2107Da.pdf
Takara
2313B
Recombinant RNase Inhibitor
°ü·ÃÇмú ±¸¸ÅÇϱâ
25,000 U (2313A x 5)
595,000¿ø 



Á¦Ç°¼³¸í
Recombinant RNase Inhibitor´Â µÅÁö °£ À¯·¡ÀÇ RNase inhibitor¸¦ ´ëÀå±Õ ¼÷ÁÖ¿¡¼­ ¹ßÇö, affinity column µîÀ» ÀÌ¿ëÇÏ¿© Á¤Á¦ÇÑ ÀçÁ¶ÇÕ ´Ü¹éÁúÀÌ´Ù. ÀÌ´Â Àΰ£ Å¹ÝÀ̳ª µÅÁö °£¿¡¼­ Á÷Á¢ Á¤Á¦ÇÑ RNase inhibitor¿Í ¸Å¿ì À¯»çÇÑ ¼ºÁúÀ» °¡Áö¸ç, RNase A¿Í 1:1 º¹ÇÕü¸¦ Çü¼ºÇÏ¿© RNaseÀÇ ÀÛ¿ëÀ» ¾ïÁ¦ÇÑ´Ù.
ÀÌ·¯ÇÑ ¹ÝÀÀÀº °¡¿ªÀûÀ̹ǷÎ, urea ¶Ç´Â sulfhydryl ½Ã¾àÀ¸·Î º¹ÇÕü¸¦ Çظ® ½ÃÅ´À¸·Î½á RNaseÀÇ È°¼ºÀ» µÇ»ì¸®°í, inhibitor¸¦ ºñ°¡¿ªÀûÀ¸·Î ½ÇÈ° ½Ãų ¼ö ÀÖ´Ù. ¶ÇÇÑ, ±âÁ¸ÀÇ ±æÇ×¼º ÀúÇØÁ¦ (nucleotide·ù, ¹«±âÀλê·ù)¿Í´Â ´Þ¸® ´Ü¹éÁúÀ̹ǷΠÆä³î 󸮷Π¹ÝÀÀ°è¿¡¼­ °£´ÜÈ÷ Á¦°ÅÇÒ ¼ö ÀÖ´Ù. ¹Ý¸é RNase HÀÇ È°¼ºÀº ÀúÇØÇÏÁö ¾Ê´Â´Ù.
ÀÌ Á¦Ç°Àº Àΰ£ Å¹ݿ¡¼­ À¯·¡ÇÑ RNase¿Í µ¿ÀÏÇÑ ¿ëµµ·Î »ç¿ë °¡´ÉÇϸç human DNA ¿À¿°ÀÌ ¿ì·ÁµÇ´Â °æ¿ì À¯¿ëÇÏ°Ô »ç¿ëÇÒ ¼ö ÀÖ´Ù
º¸Á¸ -20¡É
³óµµ 40 U/§¡
Çü»ó

20 mM

 

HEPES-KOH (pH 7.5)

50 mM

 

KCl

5 mM

 

DTT

50 %

 

Glycerol

È°¼ºÀÇ Á¤ÀÇ
5 ngÀÇ RNase A È°¼ºÀ» 50% ÀúÇØÇÏ´Â È¿¼Ò È°¼ºÀ» 1 UÀ¸·Î ÇÑ´Ù.
(ÀúÇØ È°¼ºÀº RNase A¿¡ ÀÇÇÑ Cyclic 2’, 3’-CMPÀÇ °¡¼öºÐÇظ¦ ¾ïÁ¦ÇÏ´Â ´É·Â¿¡ ÀÇÇØ Á¤·® µÇ¾ú´Ù.)
¿ëµµ
- cDNA ÇÕ¼º ½Ã (RNase inhibitor, 0.5 U/§¡ reaction)
- in vitro translation (RNase inhibitor, 1 U/§¡ reaction)
- in vitro transcription with cell-free extract (RNase inhibitor, 20 U/§¡ reaction)
- in vitro transcription with SP6 or T7 polymerase (RNase inhibitor, 1 U/§¡ reaction)
- Polysome isolation (RNase Inhibitor, 1,000 U/§¢ reaction)
±â¿ø
- µÅÁö °£ À¯·¡ÀÇ RNase inhibitor À¯ÀüÀÚ¸¦ °¡Áø plasmid¸¦ ¹ßÇöÇÏ´Â E. coli
ÀϹÝÀûÀÎ ¼ºÁú
- ºÐÀÚ·®: ¾à 52,000
- µîÀüÁ¡: pH5.0
- ÃÖÀû pH: pH6~9
- ÃÖÀû ¿Âµµ: 25¡É ~ 50¡É
- È°¼ºÈ­Á¦:
   È¯¿øÁ¦ DTT (free »óÅÂÀÇ -SH±â¸¦ º¸È£Çϱâ À§Çؼ­ storage buffer¿¡ ÃÖ¼Ò 1mMÀÇ DTT°¡ ÇÊ¿äÇÏ´Ù.)
- ¾ÈÁ¤¼º:
   ±âÆ÷ ȤÀº °­ÇÑ Ãæ°Ý¿¡ È¿¼Ò°¡ ºñÈ°¼ºÈ­ µÉ ¼ö ÀÖ´Ù.
   65¡É 15ºÐÀÇ ¿­Ã³¸®·Î ºñÈ°¼ºÈ­ ÇÒ ¼ö ÀÖ´Ù.
»ç¿ë»óÀÇ ÁÖÀÇ
- ³ÐÀº pH ¹üÀ§¿¡¼­ ¾ïÁ¦ È°¼ºÀ» º¸À̸ç pH7~8¿¡¼­ ÃÖ°íÈ°¼ºÀ» º¸ÀδÙ.
- È°¼ºÀ» ¹ßÇöÇϱâ À§Çؼ­´Â ÃÖ¼Ò 1mMÀÇ DTT°¡ ÇÊ¿äÇÏ´Ù.
- ÀÌ Á¦Ç°¿¡´Â buffer°¡ Æ÷ÇԵǾî ÀÖÁö ¾Ê´Ù.
¿ëµµ
- Ź¿ùÇÑ ½ÅÀå´É·Â ¹× Á¤È®µµ¸¦ °¡Áø ¿ªÀü»ç È¿¼Ò, PrimeScript™ Reverse Transcriptase (Code 2680A)
- cDNA ÇÕ¼ºÀ» À§ÇÑ all-in-one ÃÖ½ÅÁ¦Ç°, PrimeScript™ IV 1st strand cDNA Synthesis Mix (Code 6215A)
- °íÇ°Áú °í¼öÀ² in vitro transcriptionÀ» À§ÇÑ ½Ã½ºÅÛ, Takara IVTpro™ mRNA Synthesis System (Code 6141)
- gDNA Eraser ColumnÀ¸·Î gDNA ¿À¿° ¾øÀÌ ´Ù¾çÇÑ »ùÇÃÀÇ RNA Á¤Á¦, TaKaRa MiniBEST Universal RNA Extraction Kit (Code 9767A)

Keyword :

Reverse Transcription (Takara)
>
Premix ŸÀÔÀÇ cDNA ÇÕ¼º ½Ã¾à
PrimeScript¢â IV 1st strand cDNA Synthesis Mix
>
¾Ç¼¼»ç¸® ´Ü¹éÁúÀÌ Ãß°¡ µÈ ÃÖ»ó±Þ Full-length..
PrimeScript¢â II 1st strand cDNA Synthesis Kit
>
Ź¿ùÇÑ ½ÅÀå´É·ÂÀ» °¡Áø ½Å°³³ä ¿ªÀü»ç È¿¼ÒÀÇ ..
PrimeScript¢â Reverse Transcriptase
>
PrimeScript RTase¸¦ ÀÌ¿ëÇÑ 1st strand cDNA ..
PrimeScript¢â 1st strand cDNA Synthesis Kit
>
RT-PCR ÇʼöÇ°
Recombinant RNase Inhibitor
>
cDNA Library ±¸Ãà All-in-One Kit
cDNA Library Construction Kit
>
¿ªÀü»ç¹ÝÀÀ¿ë primer
Oligo(dT) 15 primer