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Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
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CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
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Tet One Inducible Expr..
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(Legacy) Tet-Inducible..
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Lenti-X GoStix ¾îÇà ´Ù¿î·Îµå
Lenti-X GoStix PlusÀÇ »ç¿ë ¹æ¹ý
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Applying lentiviral supernatants to Lenti-X GoStix Plus cassettes
Lenti-X GoStix Plus ½º¸¶Æ®Æù ¾îÇà »ç¿ë ¿¹¿Í GoStix Value(GV) µµÃâ
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Using the Lenti-X GoStix App to analyze the test results
±×¸² 1. Lenti-X GoStix Plus´Â Lentivirus capsid¿¡ À§Ä¡ÇÑ p24 ´Ü¹éÁúÀ» °ËÃâÇÑ´Ù.
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Lenti-X Single ShotsÀ» ÀÌ¿ëÇÏ¿© ZsGreen1 Lentivirus¸¦ 2 ¹Ýº¹ (duplicate)·Î Áغñ ÈÄ, ¹ÙÀÌ·¯½º ȸ¼ö½Ã°£¿¡ µû¶ó Infection unit°ú GV Value (GV)¸¦ ÃøÁ¤ÇÏ¿´´Ù.
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±×¸² 6. Lenti-X GoStix Plus¸¦ ÀÌ¿ëÇÑ GoStix Value(GV)´Â ELISA ¹æ½ÄÀÇ ¿ª°¡ ÃøÁ¤¹ý°ú À¯»çÇÏ´Ù
ZsGreen1 LentivirusÀÇ ¿ª°¡¸¦ ELISA ¹æ½ÄÀÇ Lenti-X p24 Rapid Titer Kit°ú Lenti-X GoStix Plus·Î ÃøÁ¤Çϰí transduction È¿À²À» FACS·Î ºÐ¼®ÇÏ¿´´Ù.
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Confirm successful lentiviral packaging reactions
Compare virus amounts between different preparations
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Lenti-X GoStix Plus
Chase Buffer
p24 Control
Citation (Lenti-X¢â qRT-PCR Titration Kit)
Identification of essential genes for cancer immunotherapy
Nature | 2017 Aug 07 | PubMed ID: 28783722 |
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DND1 maintains germline stem cells via recruitment of the CCR4-NOT complex to target mRNAs
Nature | 2017 Feb 17 | PubMed ID: 28297718 |
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Subversion of serotonin-receptor signaling in osteoblasts by kynurenine drives Acute Myeloid Leukemia
Cancer discovery | 2022 Apr 06 | PubMed ID: 35046097 |
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Therapeutic Targeting of RNA Splicing Catalysis through Inhibition of Protein Arginine Methylation
Cancer cell | 2019 Aug 12 | PubMed ID: 31408619 |
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All-optical synaptic electrophysiology probes mechanism of ketamine-induced disinhibition
Nature methods | 2018 Aug 20 | PubMed ID: 30275587 |
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The DNA modification N6-methyl-2’-deoxyadenosine (m6dA) drives activity-induced gene expression and is required for fear extinction
Nature neuroscience | 2019 Feb 18 | PubMed ID: 30778148 |
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Cand1-mediated adaptive exchange mechanism enables variation in F-box protein expression
Molecular cell | 2018 Mar 01 | PubMed ID: 29499133 |
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The TIA1 RNA-binding-protein family regulates EIF2AK2-mediated stress response and cell cycle progression
Molecular cell | 2019 Feb 15 | PubMed ID: 29429924 |
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The G3BP1-Family-USP10 Deubiquitinase Complex Rescues Ubiquitinated 40S Subunits of Ribosomes Stalled in Translation from Lysosomal Degradation.
Molecular cell | 2020 Jul 14 | PubMed ID: 31981475 |
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Overexpression of the base excision repair NTHL1 glycosylase causes genomic instability and early cellular hallmarks of cancer
Nucleic Acids Research | 2018 May 18 | PubMed ID: 29522130 |
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Nucleic acid binding proteins affect the subcellular distribution of phosphorothioate antisense oligonucleotides
Nucleic Acids Research | 2017 Oct 13 | PubMed ID: 28977508 |
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The human RecQ helicases BLM and RECQL4 cooperate to preserve genome stability
Nucleic Acids Research | 2012 Aug 01 | PubMed ID: 22544709 |
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NASP maintains histone H3–H4 homeostasis through two distinct H3 binding modes
Nucleic Acids Research | 2022 May 20 | PubMed ID: 35489058 |
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A selective chemical probe for exploring the role of CDK8 and CDK19 in human disease
Nature chemical biology | 2015 Oct 26 | PubMed ID: 26502155 |
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LETR1 is a lymphatic endothelial-specific lncRNA governing cell proliferation and migration through KLF4 and SEMA3C
Nature Communications | 2021 Feb 10 | PubMed ID: 33568674 |
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miR-130a and miR-145 reprogram Gr-1+CD11b+ myeloid cells and inhibit tumor metastasis through improved host immunity
Nature Communications | 2018 Jul 04 | PubMed ID: 29973593 |
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A high-throughput screen for TMPRSS2 expression identifies FDA-approved compounds that can limit SARS-CoV-2 entry
Nature Communications | 2021 Jun 23 | PubMed ID: 34162861 |
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A single-cell survey of the human first-trimester placenta and decidua
Science Advances | 2018 Oct 01 | PubMed ID: 30402542 |
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Integrating flexible electronics for pulsed electric field delivery in a vascularized 3D glioblastoma model
npj Flexible Electronics | 2021 Aug 13 |
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LAG3 is not expressed in human and murine neurons and does not modulate α
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synucleinopathies
EMBO Molecular Medicine | 2021 Jul 26 | PubMed ID: 34309222 |
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p53 pulse modulation differentially regulates target gene promoters to regulate cell fate decisions
Molecular Systems Biology | 2019 Sep 26 | PubMed ID: 31556489 |
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Coordination of MAPK and p53 dynamics in the cellular responses to DNA damage and oxidative stress
Molecular Systems Biology | 2022 Dec 06 |
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Rapid neurogenesis through transcriptional activation in human stem cells
Molecular Systems Biology | 2014 Nov 01 | PubMed ID: 25403753 |
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Self-targeting of TNF-releasing cancer cells in preclinical models of primary and metastatic tumors
Proceedings of the National Academy of Sc… | 2016 Feb 23 | PubMed ID: 26858439 |
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Keratins as the main component for the mechanical integrity of keratinocytes
Proceedings of the National Academy of Sc… | 2013 Nov 12 | PubMed ID: 24167246 |
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Age-related loss of neural stem cell O-GlcNAc promotes a glial fate switch through STAT3 activation
Proceedings of the National Academy of Sc… | 2020 Sep 08 | PubMed ID: 32848054 |
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Evolutionary history and metabolic insights of ancient mammalian uricases
Proceedings of the National Academy of Sc… | 2014 Mar 11 | PubMed ID: 24550457 |
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Hydrogen peroxide differentially modulates cardiac myocyte nitric oxide synthesis
Proceedings of the National Academy of Sc… | 2011 Sep 20 | PubMed ID: 21896719 |
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Human SLFN5 is a Transcriptional Co-repressor of STAT1-Mediated Interferon Responses and Promotes the Malignant Phenotype in Glioblastoma
Oncogene | 2017 Jul 03 | PubMed ID: 28671669 |
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Rucaparib Treatment Alters p53 Oscillations in Single Cells to Enhance DNA-Double-Strand-Break-Induced Cell Cycle Arrest
Cell reports | 2020 Oct 13 | PubMed ID: 33053351 |
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Keyword : Lentivirus,·»Æ¼¹ÙÀÌ·¯½º,¿ª°¡,Titer,Titration,Stix,Gostix,·»Æ¼,¹ÙÀÌ·¯½º,Á¤·®,soeasy,so easy
Lentivirus
[°³¿ä] Packaging Single Shots
[FAQ] Lentiviral Gene Delivery
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
SARS-CoV-2-pseudotyped lentivirus »ý»ê
Lenti-X¢â SARS-CoV-2 Packaging Mix
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3¼¼´ë Lentivirus packaging system
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Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
Lentivirus¸¦ ÀÌ¿ëÇÑ sgRNA, Cas9 gene deliver..
Lenti-X¢â CRISPR/Cas9 System
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
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Reporter assay ½Ã½ºÅÛ (Promoterless vector)
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Packaging single shots
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Packaging Cell Lines
1 step qRT-PCR·Î ¿ª°¡¸¦ ºü¸£°í Á¤È®ÇÏ°Ô ÃøÁ¤
Lenti-X¢â qRT-PCR Titration Kit
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
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Provirus Quantitation Kit
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
ÃÖÀûÈµÈ Transfection Reagent & Packaging Mi..
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IT¢ç Lentivirus System