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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Lentivirus > Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
p24 ELISA·Î ºü¸£°í Á¤È®ÇÏ°Ô ¿ª°¡ ÃøÁ¤

Lenti-X¢â p24 Rapid Titer Kit (Single Wash)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631476
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 ȸ
972,000¿ø 
1,215,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x345243, x345242
Clontech
632200
Lenti-X¢â p24 Rapid Titer Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 ȸ
970,400¿ø 
1,213,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x32776, x32777

  • ELISA¹ýÀ¸·Î °£´ÜÇÏ°Ô lentivirus ¾ç ÃøÁ¤ (titration)
  • ºÐ¸®°¡ °¡´ÉÇÑ 12 X 8-well strip ÇüÅÂÀÇ 96-well·Î ½ÇÇè¿¡ µû¶ó flexibleÇÏ°Ô Àû¿ë °¡´É
  • º¸´Ù ½Å¼ÓÇÏ°Ô °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Â Single Wash ŸÀÔ (~1.5 h) Ãâ½Ã

Virus titrationÀÌ ÇÊ¿äÇÑ ÀÌÀ¯
    1. Lentivirus Packaging °úÁ¤ÀÌ Á¦´ë·Î µÇ¾ú´ÂÁö È®ÀÎ
    2. ¿ÀÂ÷¾øÀÌ ÀÏ°üµÈ ½ÇÇè¼öÇà
    3. 100% TransductionÀ» À§ÇÑ ÃæºÐÇÑ MOI °áÁ¤
    4. Copy number Á¶ÀýÀ» ÅëÇÑ À¯ÀüÀÚ ¹ßÇö¾ç Á¶Àý

Á¦Ç°¼³¸í
Lentivirus´Â ÇöÀç ´Ù¾çÇÑ ¸ñÀûÀ¸·Î Cell & Gene therapy ºÐ¾ß¿¡¼­ »ç¿ëµÇ°í ÀÖ´Ù. ÀÌ ¹ÙÀÌ·¯½º´Â gag À¯ÀüÀÚ¿¡ ÀÇÇØ ¹ßÇöµÇ´Â capsid ´Ü¹éÁú (p24), nucleocapsid ´Ü¹éÁú (p6 ¹× p7), matrix ´Ü¹éÁú (p17) µîÀ» °¡Áö°í ÀÖ´Ù. ÀÌ Áß capsid ´Ü¹éÁúÀÎ p24 (±×¸² 1)ÀÇ ³óµµ´Â lentivirusÀÇ ½ÇÁ¦ ¿ª°¡¿Í ³ôÀº »ó°ü°ü°è¿¡ ÀÖ´Ù. Lenti-X¢â p24 Rapid Titer Kit´Â ¹è¾ç »óµî¾×ÀÇ lentivirus°¡ °¡Áø p24 ´Ü¹éÁúÀÇ ¾çÀ» ÃøÁ¤ÇÏ´Â ELISA KitÀÌ´Ù.

±×¸² 1. Lentivirus ±¸Á¶
p24 ´Ü¹éÁúÀº HIV-1 À¯·¡ÀÇ gag À¯ÀüÀÚ¿¡¼­ ¹ßÇöµÇ´Â 4°³ÀÇ ´Ü¹éÁú Áß Çϳª·Î lentivirusÀÇ capsid¿¡ Á¸ÀçÇÑ´Ù.
Lenti-X¢â p24 Rapid Titer Kit (Single Wash), Lenti-X¢â p24 Rapid Titer Kit ºñ±³

Lenti-X¢â p24 Rapid Titer Kit (Single Wash)

Lenti-X¢â p24 Rapid Titer Kit

Code

631476

632200

2 Â÷ Ç×ü

HRP ¶óº§¸µ

Biotin ¶óº§¸µ

½ÇÇè ½Ã°£

1.5 ½Ã°£

3 ½Ã°£

Àüü ½ÇÇè °úÁ¤

4 ´Ü°è

8 ´Ü°è

Wash Ƚ¼ö

1 ¹ø

3 ¹ø

p24 ÃøÁ¤ ¹üÀ§

100~1,600 pg/§¢

12.5~200 pg/§¢



±×¸² 2. Lenti-X¢â p24 Rapid Titer Kit (Single Wash)ÀÇ workflow

¹è¾ç »óµî¾×, Lysis Buffer ¹× Anti-p24 (HRP Conjugate)¸¦ Æ÷ÇÔÇÑ »ùÇÃÀ» Anti-p24 Coated PlateÀÇ well¿¡ ÷°¡ÇÏ¿© incubationÇÑ´Ù. ÀÌÈÄ washÇÏ°í ¹ß»ö ½Ã¾àÀ» ÷°¡Çϸé p24 ´Ü¹éÁúÀ» Á¤·®ÇÒ ¼ö ÀÖ´Ù.
¹ÙÀÌ·¯½ºÀÇ °¨¿°¼º°ú p24 titrationÀÇ »ó°ü°ü°è
ELISA¸¦ »ç¿ëÇÑ titration kit´Â »ç¿ëÀÌ Æí¸®ÇÏ°í ÀçÇö¼ºÀÌ ³ôÀ¸¸ç ªÀº ½Ã°£ ¾È¿¡ Á¤È®ÇÑ °á°ú¸¦ Á¦°øÇϱ⠶§¹®¿¡ ¹ÙÀÌ·¯½ºÀÇ titration¿¡ ÀÌ»óÀûÀÌ´Ù. ¶ÇÇÑ ¹ÙÀÌ·¯½º stockÀÇ functional titer (IFU/§¢)À» º°µµÀÇ µ¶¸³ÀûÀÎ ¹æ¹ýÀ¸·Î °°ÀÌ ÃøÁ¤Çϸé p24 titer (ng/§¢)¿Í functional titerÀÇ °è¼ö (IFU/ng)¸¦ ±¸ÇÒ ¼ö ÀÖ´Ù. ÀÌ °è¼ö¸¦ È°¿ëÇϸé ÃßÈÄ ½ÇÇè¿¡¼­ ªÀº ½Ã°£³»¿¡ ¾òÀº p24 titer¸¦ È°¿ëÇØ functional titer (IFU/§¢)¸¦ ÃßÁ¤ÇÒ ¼ö ÀÖ°í À̸¦ ¹ÙÅÁÀ¸·Î MOI¸¦ °è»êÇÒ ¼ö ÀÖ´Ù.
³»¿ë
Lenti-X¢â p24 Rapid Titer Kit (Single Wash) (Code 631476)
   Anti-p24 Coated Plate (96 well)

1 °³

   p24 Control

500 ng

   p24 Reconstitution Buffer

1.5 §¢

   Diluent Buffer

20 §¢

   Anti-p24 (HRP Conjugate)

12 §¢

   Lysis Buffer

4 §¢

   Washing Buffer (20X)

50 §¢

   TMB Substrate

12 §¢

   Stop Solution

12 §¢


Lenti-X¢â p24 Rapid Titer Kit (Code 632200)
   Anti-p24 Coated Plate (96 wells)

1 °³

   p24 Control (10 ng/§¢)

100 §¡

   Lysis Buffer

6 §¢

   Anti-p24 (Biotin Conjugate)

12 §¢

   Streptavidin HRP

12 §¢

   Wash Buffer (20X)

120 §¢

   TMB Substrate

12 §¢

   Stop Solution

12 §¢

º¸Á¸
Lenti-X¢â p24 Rapid Titer Kit (Single Wash)
  • 4¡É º¸°ü
  • 20X Wash Buffer´Â ù »ç¿ë ÈÄ ½Ç¿Â º¸°ü

Lenti-X¢â p24 Rapid Titer Kit
  • 4¡É º¸°ü
°ü·ÃÁ¦Ç°
- FunctionalÇÑ ¹ÙÀÌ·¯½º¸¦ ´õ ¸¹ÀÌ »ý»êÇÏ´Â transfection ½Ã¾à TransIT-VirusGEN¢ç Transfection Reagent (Code MIR 6700)
- 10ºÐ¸¸¿¡ ³¡³ª´Â lentivirus ¿ª°¡ °£ÀÌ ÃøÁ¤ Kit Lenti-X¢â GoStix¢â Plus (Code 631280)
- qPCRÀ» »ç¿ëÇÑ Á¤È®ÇÑ lentivirus titration Kit Lenti-X¢â qRT-PCR Titration Kit (Code 631235)
- Lentivirus ¿ª°¡°¡ ³·´Ù¸é ¹ö¸®Áö ¸»°í ³óÃàÇؼ­ »ç¿ë Lenti-X¢â Concentrator (Code 631231)
- Functional titer¸¦ °¡Àå Á¤È®ÇÏ°Ô ÃøÁ¤ °¡´ÉÇÑ ¹æ¹ý Lenti-X¢â Provirus Quantitation Kit (Code 631239)
- Àúµ¶¼º°ú °íÈ¿À²À» Çѹø¿¡ ÀâÀº transduction enhancer RetroNectin¢ç (Code T100A ¿Ü)




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