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Home > ÀüÁ¦Ç°º¸±â > ´Ü¹éÁú ¹ßÇö ¡¤ Á¤Á¦ > ´ëÀå±Õ ´Ü¹éÁú ¹ßÇö > ´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ Á¤Á¦ Starter Kit

´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ Á¤Á¦ Starter Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
631428
pET Express & Purify Kit-His60 (In-Fusion¢ç Ready)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20ȸ
(Package)
1,855,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32711, x32907
Clontech
631429
pET Express & Purify Kit-HisTALON (In-Fusion¢ç Ready)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20ȸ
(Package)
1,814,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32712, x32713, x32714, x32907
Clontech
631430
pET Express & Purify Kit-HisTALON
°ü·ÃÇмú ±¸¸ÅÇϱâ
20ȸ
1,711,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32712, x32713, x32714, x32907
Clontech
631431
pET Express & Purify Kit-His60
°ü·ÃÇмú ±¸¸ÅÇϱâ
20ȸ
(Package)
1,751,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32711, x32907


  • His-tagged proteinÀÇ ÃÖÀûÈ­µÈ ¹ßÇö°ú Á¤Á¦
  • °°Àº kit ¾È¿¡ Á¦°øµÇ´Â N-¸»´Ü°ú C- ¸»´Ü 6 x HN-tagged vector
  • T7 lac promoter¸¦ ÅëÇØ À¯µµ °¡´ÉÇÑ pET ±â¹ÝÀÇ ´Ü¹éÁú ¹ßÇö ½Ã½ºÅÛ
  • Cloning ¹æ¹ý, Á¤Á¦¿ë resin Á¾·ù¿Í resin ÇüŸ¦ ¼±Åà °¡´É
  • CEP kit¸¦ »ç¿ëÇÑ ½±°í, °íÈ¿À²ÀÇ ÀçÇö¼ºÀÖ´Â cloning
Bacterial Expression & Purification Starter Kit´Â cloning ÇüŸ¦ ¼±ÅÃÇÒ ¼ö ÀÖµµ·Ï Á¦°øµÇ¸ç N-¸»´ÜÀ̳ª C-¸»´Ü 6 x HN fusion tagÀ» encodingÇÏ´Â pET ±â¹Ý vector (1)¸¦ »ç¿ëÇÏ¿© histidine Ç¥Áö ´Ü¹éÁúÀ» °í¹ßÇö½Ãų ¼ö ÀÖ´Ù.
¸ñÀû À¯ÀüÀÚ´Â pEcoli expression vector·Î cloningÇÏ¿© N-¸»´ÜÀ̳ª C-¸»´Ü¿¡ 6 x HN tagµÈ ±¸Á¶¸¦ ¸¸µé¼ö ÀÖ´Ù.
Cloning ¹æ¹ý ¼±ÅÃ
EP (Express & Purify) Starter Kit´Â Á¦ÇÑÈ¿¼Ò¸¦ »ç¿ëÇÏ´Â ÀϹÝÀûÀÎ cloning¿ë circular vector¸¦ Æ÷ÇÔÇÏ´Â pEcoli Expression SystemÀ¸·Î ±¸¼ºµÇ¾î ÀÖ´Ù. Vector¿¡´Â MSC site°¡ ÀÖ¾î ¿øÇÏ´Â ¼­¿­¿¡ ¸ÂÃç cloningÇÒ ¼ö ÀÖ´Ù.
CEP (Clone, Express & Purify) Starter Kit´Â cloningÀ» À§ÇØ linearized vector¿Í In-Fusion È¿¼Ò¸¦ Á¦°øÇÏ´Â pEcoli Expression SystemÀ» Æ÷ÇÔÇÑ´Ù. ÀÌ ½Ã½ºÅÛÀº ClontechÀÇ In-Fusion cloning ±â¼úÀ» »ç¿ëÇÏ¿© ½±°í, È®½ÇÇÏ°Ô PCR directional cloningÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù (2). »ý¹°ÇÐÀû È°¼ºÀ» À¯Áö½ÃÅ°±â À§ÇØ ¸ñÀû À¯ÀüÀÚ¿¡ ¿°±â¸¦ Ãß°¡ÇÏÁö ¾Ê¾Æµµ µÇ´Â Å« ÀåÁ¡ÀÌ ÀÖ´Ù.
Á¤Á¦ resin ¼±ÅÃ
E. coli ¿¡ ÇüÁúÀüȯ°ú ¼±º°°úÁ¤ ÈÄ, ¹ßÇöµÈ N-¸»´ÜÀ̳ª C-¸»´Ü Ç¥Áö ´Ü¹éÁúÀº immobilized metal affinity chromatography (IMAC) resin°ú buffer¸¦ ¼±ÅÃÇÏ¿© È¿°úÀûÀ¸·Î Á¤Á¦ÇÑ´Ù. °¡Àå ÁÁÀº °áÇÕ·ÂÀ» À§ÇØ His60 Ni Superflow ResinÀ̳ª °¡Àå ÁÁÀº Á¤Á¦ ¼øµµ¸¦ À§Çؼ± HisTALON ResinÀ» ¼±ÅÃÇÒ ¼ö ÀÖ´Ù. ¸ðµç EP¿Í CEP Starter Kit´Â ´Ü¹éÁú ÃßÃâ°ú Á¤Á¦¸¦ À§ÇØ ÇÊ¿äÇÑ buffer³ª resinÀÇ Á¾·ù, resinÀÇ ÇüÅ¿¡ µû¶ó Á¦Ç°À» ¼±ÅÃÇÒ ¼ö ÀÖ´Ù.


Figure 1. Excellent purity is obtained when using HisTALON Cartridges for purification. The CEP Starter Kit was used to generate BL21 E. coli expressing N-terminal 6xHN-tagged AcGFP1, as seen in Panels A & B; and C-terminal 6xHN-tagged AcGFP1, as seen in Panels C & D.


Figure 2. Use His60 Ni Superflow for the highest binding capacity.The CEP His60 Ni Superflow Starter Kit was used to generate and purify 6xHNAcGFP1. Lane M: Molecular weight marker. Lane OS: Clarified lysate with expressed 6xHN-AcGFP1. Lane FT: Nonadsorbed material. Lanes W1 and W2: Wash. Lane E1-E4: Eluted 6xHN-AcGFP1 fractions.


Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Moffatt, B.A. and Studier, F.W. (1986) J. Mol.Biol. 189(1):113-130.
2. In-Fusion PCR Universal Cloning Kits (2005) Clontechniques XX(2):16-17.
´ëüǰ ¾È³»

Á¾¸ÅÇ°

´ëüǰ

EP His60 Ni Gravity Column Starter Kit (Code 631416)

pET Express & Purify Kit-HisTALON (Code 631430)

EP HisTALON Gravity Column Starter Kit (Code 631319)

pET Express & Purify Kit-His60(Code 631431)

CEP HisTALON Gravity Column Starter Kit (Code 631423)

pET Express & Purify Kit-His60 (In-Fusion Ready) (Code 631428)

CEP His60 Ni Gravity Column Starter Kit (Code 631422)

pET Express & Purify Kit-HisTALON (In-Fusion Ready)(Code 631429)


Keyword : 631416,631433,635657,635665, 631419, 631423, 631422

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ChaperoneÀ» ¹ßÇöÇÏ´Â E. coli BL21 Competent ..
Chaperone Competent Cell BL21 ½Ã¸®Áî
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ÀûÀýÇÑ foldingÀ» À§ÇÑ chaperone team
Chaperone Plasmid Set
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GST TagÀÌ Æ÷ÇÔµÈ Cold shock Vector
pCold¢â GST DNA
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Soluble tagÀÇ °áÁ¤ÆÇ
pCold¢â ProS2 DNA
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Soluble tag (TF tag) ÀÌ Æ÷ÇÔµÈ Cold shock Ve..
pCold¢â TF DNA
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°íÈ¿À² E. coli Cold shock ¹ßÇö½Ã½ºÅÛ
pCold¢â Vector ½Ã¸®Áî
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Single Protein Production System (SPP System¢â)