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NGS °ü·Ã
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Stranded RNA-seq
> Single cell Stranded Kit
´ÜÀϼ¼Æ÷ (single cell)ÀÇ stranded RNA-Seq library kit
Single cell Stranded Kit
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Clontech
634442
SMART-Seq¢ç Stranded Kit
12 ȸ
1,592,000¿ø
1,990,000¿ø
11.01 ~ 12.29
Clontech
634443
SMART-Seq¢ç Stranded Kit
48 ȸ
5,284,800¿ø
6,606,000¿ø
11.01 ~ 12.29
Clontech
634444
SMART-Seq¢ç Stranded Kit
96 ȸ
6,997,600¿ø
8,747,000¿ø
11.01 ~ 12.29
Product index
PGT-A
Embgenix¢â PGT-A Kit (..
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸..
mRNA-Seq (Single cell)
Single cell RNA-seq¿¡ ..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
´Ù·®ÀÇ ±Ø¼Ò·® »ùÇÃÀ» ..
±Ø¼Ò·® mRNAÀÇ 3¡¯ ¸»´Ü..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
´Ù·®ÀÇ ±Ø¼Ò·® »ùÇÃÀ» ..
Single cell RNA-seq¿¡ ..
Stranded RNA-seq
Single cell Stranded K..
[NEW] Pico Input Mamma..
Pico Input Mammalian (..
Low Input Mammalian
HI Mammalian
Stranded RNA-Seq Kit
Total RNA-Seq Kit (FFP..
Small RNA-Seq
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[Version 2] Human TCR ..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
SMART-Seq Human BCR (w..
SMART-Seq Human TCR (w..
SMART-Seq¢ç Mouse TCR ..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
ChIP-Seq / Meth-Seq
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
Unique Dual Index Kits
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) SMARTer Ultra..
(Legacy) Human TCR a/b..
(Legacy) PicoPLEX¢ç DN..
(Legacy) ThruPLEX¢ç Pl..
(Legacy) ThruPLEX¢ç DN..
(Legacy) PicoPLEX¢ç WG..
1 ~ 1000°³ÀÇ ¼¼Æ÷ ¶Ç´Â ±Ø¼Ò·®ÀÇ total RNA (10pg - 10ng)À¸·ÎºÎÅÍ 7½Ã°£ ³» Illumina
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library Á¦ÀÛ
Single cell ¼öÁØÀÇ ¹Î°¨µµ·Î ¹æÇ⼺ Á¤º¸ (strand origin information)¸¦ Æ÷ÇÔÇÑ full-length total RNA sequencing
Single cell ¶Ç´Â total RNA·ÎºÎÅÍ coding/non-coding transcript¸¦ ÀçÇö¼º°ú Á¤È®¼º ³ô°Ô ºÐ¼®
ºÐÇØµÈ low quality RNA¿¡µµ Àû¿ë °¡´É
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SMART-Seq
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Stranded KitÀº 1 - 1,000°³ÀÇ ¼¼Æ÷ ¶Ç´Â 10pg - 10ngÀÇ total RNA·ÎºÎÅÍ ¹æÇ⼺ Á¤º¸ (strand-of origin information)¸¦ °¡Áø Illumina
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NGS Library¸¦ Á¦ÀÛÇÏ´Â Á¦Ç°ÀÌ´Ù. SMART (Switching Mechanism at 5' End of RNA Template) ±â¼úÀ» ÀÌ¿ëÇÏ¿© Full-length transcript¿¡ ´ëÇÑ NGS ºÐ¼®ÀÌ °¡´ÉÇϸç, library Á¦ÀÛ ´Ü°è¿¡¼ rRNA À¯·¡ÀÇ cDNA¸¦ Á¦°ÅÇÏ´Â ZapR ±â¼úÀÌ Àû¿ëµÇ¾îÀÖ¾î º°µµÀÇ rRNA Á¦°Å ½Ã¾àÀÌ ÇÊ¿äÇÏÁö ¾Ê´Ù.
º» Á¦Ç°Àº Random-primingÀ» ÀÌ¿ëÇÏ¿© ¿ªÀü»ç ¹ÝÀÀÀ» ÁøÇàÇϹǷÎ, ǰÁúÀÌ ÁÁÁö ¾ÊÀº ºÐÇØµÈ RNA »ùÇ÷κÎÅÍ NGS Library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. High-quality RNA·ÎºÎÅÍ Oligo dT primer¸¦ ÀÌ¿ëÇÑ NGS Library Á¦ÀÛÀ» ÁøÇà ¿¹Á¤À̶ó¸é
SMART-Seq
¢ç
v4 Ultra
¢ç
Low Input RNA Kit for Sequencing
(Code 634888)ÀÇ »ç¿ëÀ» ±ÇÀåÇÑ´Ù.
±×¸² 1. SMART-Seq
¢ç
Stranded Kit Workflow
±×¸² 2. Structural features of final libraries generated with the SMART-Seq Stranded Kit.
The adapters added during PCR1 contain sequences allowing clustering on Illumina flow cells (P7 shown in light blue, P5 shown in red), indexes for pooling multiple samples in a single sequencing lane (Index 1 [i7] sequence shown in orange and associated with the P7 sequence, and Index 2 [i5] sequence shown in orange and associated with P5 sequence), as well as the regions recognized by sequencing primers Read Primer 2 (Read 2, purple) and Read Primer 1 (Read 1, green). Read 1 generates sequences antisense to the original RNA, while Read 2 yields sequences sense to the original RNA (orientation of original RNA denoted by 5¡¯ and 3¡¯ in dark blue). The first three nucleotides of the second sequencing read (Read 2) are derived from the SMART-Seq Stranded Adapter (shown as Xs). It is best to trim these three nucleotides prior to mapping if performing paired-end sequencing.
Ç¥ 1. Consistent sequencing metrics across RNA input amounts.
Human brain total RNA (10 pg-10 ng) was used to generate RNA-seq libraries with the SMART-Seq Stranded Kit. Data shown are the average of three technical replicates and exhibit exceptionally high Pearson and Spearman correlations between replicates, even with as little as 10 pg of input material. Sequences were analyzed as described in the Methods.
±×¸² 3. The SMART-Seq Stranded Kit outperforms the Pico v2 kit for ultra-low inputs.
Panel A. Human brain total RNA (50 pg-10 ng) was used to generate RNA-seq libraries in triplicate with the SMART-Seq Stranded Kit (Stranded) and the Pico v2 kit. For both kits, sequencing data were down-sampled to 2.5 million paired-end reads prior to analysis. Panel B . Comparison of transcript expression level from libraries generated with 50 pg of total RNA (as shown in Panel A). FPKM values are shown on a log10 scale. Transcripts represented in only one sample (dropouts) can be seen along the X- and Y-axes of the scatter plots.
±×¸² 4. High reproducibility across cell input amounts. A375 cells isolated by FACS were used to generate RNA-seq libraries with the SMART-Seq Stranded Kit.
Input varied from 1 cell to 1,000 cells, with two replicates per input of 5-1,000 cells and 12 replicates for the single cells. For comparison, two aliquots of 1,000 cells were used for total RNA purification and then used for library preparation. Panel A . Consistent sequencing metrics across 1-1,000 cells. Panel B. Hierarchical clustering heat map displaying Euclidean distance between all the samples shown in Panel A, and reporting Pearson correlations ranging from 0.85 to 0.99. Single cells are labelled Cell1-Cell12; replicates for other inputs are labelled a-b.
Ç¥ 2. Features comparison of commercially available single-cell RNA-Seq Kits.
±×¸² 5. Comparison between the SMART-Seq Stranded Kit and the QIAseq FX Single Cell RNA Library Kit
. Panel A. Human brain total RNA (50 pg) and cells isolated by FACS (Jurkat cell line, 50 cells) were used to generate RNA-seq libraries in duplicate with the SMART-Seq Stranded Kit (Stranded) and the QIAseq FX Single Cell RNA Library Kit (QIAseq FX). Following sequencing, human brain total RNA data were analyzed using 2.5M paired-end reads for the Stranded kit, and 5M paired-end reads for the QIAseq FX kit. All data generated from Jurkat cells were normalized to 8M paired-end reads. The Pearson and Spearman correlations were determined between the replicates shown. Panel B . A downsampling experiment with the data generated from Jurkat cells clearly shows that the higher sensitivity observed for the Stranded kit is maintained with lower sequencing depth. Panel C. Assessment of reproducibility between the two kits for the libraries generated from Jurkat cells. Using an expression level cutoff of FPKM >1, the total number of transcripts identified and the overlap between the replicates is much greater for the Stranded kit.
Àû¿ë
Library generation from small quantities of cells (1-1,000 cells) or total RNA (10 pg-10 ng) for transcriptome sequencing with strand-of-origin information
Generation of sequencing-ready Illumina libraries in 7 hours
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SMART-Seq Stranded Kit Components
Indexing Primer Set HT for Illumina v2
Keyword : Single cell,´ÜÀϼ¼Æ÷,½Ì±Û¼¿,single cell analysis,single cell NGS,full length,total RNA,ZapR,rRNA,Strand specific,Stranded-Seq,cDNA,cDNA-seq,NGS,Next Generation Sequencing,SMART,SMARTer,smart-seq
Stranded RNA-seq
´ÜÀϼ¼Æ÷ (single cell)ÀÇ stranded RNA-Seq li..
Single cell Stranded Kit
UDI, UMI Àû¿ëÀ¸·Î ´õ¿í Ưº°ÇØÁø
[NEW] Pico Input Mammalian (v3)
Picogram RNA·ÎºÎÅÍ Strand-specific illumina ..
Pico Input Mammalian (v2)
Low Input Mammalian
High Input Strand- Specific Total RNA-Seq
HI Mammalian
Stranded RNA-Seq Kit
Total RNA-Seq Kit (FFPE, LCM »ùÇà ºÐ¼®)