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NGS °ü·Ã
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Stranded RNA-seq
> [NEW] Pico Input Mammalian (v3)
UDI, UMI Àû¿ëÀ¸·Î ´õ¿í Ưº°ÇØÁø
[NEW] Pico Input Mammalian (v3)
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
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(ºÎ°¡¼¼º°µµ)
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Clontech
634485
SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian
24 ȸ
3,661,600¿ø
4,577,000¿ø
11.01 ~ 12.29
Clontech
634486
SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian
48 ȸ
5,825,600¿ø
7,282,000¿ø
11.01 ~ 12.29
Clontech
634487
SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian
96 ȸ
7,676,800¿ø
9,596,000¿ø
11.01 ~ 12.29
Clontech
634488
SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian
192 ȸ
13,723,200¿ø
17,154,000¿ø
11.01 ~ 12.29
Product index
PGT-A
Embgenix¢â PGT-A Kit (..
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸..
mRNA-Seq (Single cell)
Single cell RNA-seq¿¡ ..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
´Ù·®ÀÇ ±Ø¼Ò·® »ùÇÃÀ» ..
±Ø¼Ò·® mRNAÀÇ 3¡¯ ¸»´Ü..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
´Ù·®ÀÇ ±Ø¼Ò·® »ùÇÃÀ» ..
Single cell RNA-seq¿¡ ..
Stranded RNA-seq
Single cell Stranded K..
[NEW] Pico Input Mamma..
Pico Input Mammalian (..
Low Input Mammalian
HI Mammalian
Stranded RNA-Seq Kit
Total RNA-Seq Kit (FFP..
Small RNA-Seq
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[Version 2] Human TCR ..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
SMART-Seq Human BCR (w..
SMART-Seq Human TCR (w..
SMART-Seq¢ç Mouse TCR ..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
ChIP-Seq / Meth-Seq
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
Unique Dual Index Kits
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) SMARTer Ultra..
(Legacy) Human TCR a/b..
(Legacy) PicoPLEX¢ç DN..
(Legacy) ThruPLEX¢ç Pl..
(Legacy) ThruPLEX¢ç DN..
(Legacy) PicoPLEX¢ç WG..
* º» Á¦Ç°Àº
SMARTer¢ç Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Code 634411)
ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°ÀÔ´Ï´Ù.
* º» Á¦Ç°Àº 24, 48, 96, 192ȸ Á¦Ç°À¸·Î Á¦°øµÇ¸ç,
SMARTer RNA Unique Dual Index Kit - 24U (Code 634451, 96ȸ)
°¡ Æ÷ÇԵǾî ÀÖ½À´Ï´Ù.
SMARTer¢ç RNA Unique Dual Index Kit - 96U Set A/B (Code 634452/634457, °¢ 96ȸ)
À» º°µµ·Î ±¸¸ÅÇϸé ÃÖ´ë 192°³ÀÇ ´Ù¸¥ index¸¦ Á¶ÇÕÇÑ library¸¦ ±¸ÃàÇÒ ¼ö ÀÖ½À´Ï´Ù.
pg ¼öÁØÀÇ ±Ø¼Ò·® »ùÇà Àû¿ë
- Human, mouse, rat À¯·¡ÀÇ total RNA (250 pg - 10 ng) ȤÀº intact cell (10-1,000°³)
UMI Àû¿ëÀ¸·Î ºÐ¼® Á¤È®µµ Áõ°¡
- »ùÇà ³» true variant¿Í rare mutationÀÇ Á¤È®ÇÑ ºÐ¼® ¹× PCR ÁõÆø°úÁ¤À¸·Î ¹ß»ýÇÒ ¼ö ÀÖ´Â bias³ª sequencing error ¹æÁö
FFPE³ª LCM, cell-free RNA¸¦ Æ÷ÇÔÇÏ´Â ³·Àº quality RNA¿¡¼ ÀçÇö¼º ³ôÀº °á°ú È®ÀÎ
ºü¸£°í °£ÆíÇÑ ÇÁ·ÎÅäÄÝ
- Buffer Á¶¼ºÀÇ ¾÷±×·¹À̵å·Î library Á¤Á¦ ½Ã°£ °¨Ãà, Àü °úÁ¤ÀÌ 7.5½Ã°£ ³» ¿Ï·á
Illumina¢ç Ç÷§Æû ȣȯ
- Illumina¢ç ºÐ¼®¿ë index°¡ Àû¿ëµÇ¾î ÀÖÀ¸¸ç, ÃÖ´ë 192°³ÀÇ Á¶ÇÕ °¡´É
Á¦Ç° ¼³¸í
SMARTer¢ç Stranded Total RNA - Seq Kit v3 - Pico Input Mammalian´Â human, rat, mouse À¯·¡ÀÇ total RNA (250 pg ~ 10 ng) ȤÀº intact cell (10-1,000°³)·ÎºÎÅÍ Strand-specific Illumina¢ç NGS library¸¦ È¿À²ÀûÀ¸·Î Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº ±Ø¹Ì·®ÀÇ total RNA¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖÀ» »Ó¸¸ ¾Æ´Ï¶ó FFPE, LCM »ùÇðú °°ÀÌ RNA integrity°¡ ÇöÀúÈ÷ ³·Àº »ùÇÿ¡¼µµ NGS Library Á¦ÀÛÀÌ °¡´ÉÇÏ´Ù. º» Á¦Ç°Àº library Á¦ÀÛ °úÁ¤ ³»¿¡ unique molecular identifiers (UMIs)¸¦ Ãß°¡ µµÀÔÇÔÀ¸·Î½á ±âÁ¸ÀÇ Version 2 Á¦Ç°¿¡ ºñÇØ Illumina¢ç Sequencing È¿À²°ú Á¤È®µµ¸¦ °³¼±ÇÏ¿´´Ù.
SMARTer¢ç Stranded Total RNA - Seq Kit v3 - Pico Input Mammalian¸¦ ÀÌ¿ëÇϸé, ¿ªÀü»ç¹ÝÀÀ°ú PCR ÁõÆø ´Ü°è¿¡¼ index¿Í adapter¸¦ ºÎ°¡Çϸç, strand Á¤º¸¸¦ º¸À¯ÇÏ´Â library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. Library prep ¹× Á¤Á¦ °úÁ¤À» Æ÷ÇÔÇÑ Àü ½ÇÇè °úÁ¤Àº 7.5½Ã°£ ³» ¿Ï·áµÈ´Ù.
º» Á¦Ç°Àº Mammalian rRNA¿Í mitochondrial RNA ƯÀÌÀûÀÎ Probe·Î rRNA derived cDNA¸¦ Á¦°ÅÇÏ¿© º¸´Ù ³ôÀº mapping statistics¸¦ ¾òÀ» ¼ö ÀÖ´Â ±â¼úÀÌ Àû¿ëµÇ¾î ÀÖ´Ù. Ribosomal RNA (rRNA)´Â total RNAÀÇ ¾à 90%¸¦ Â÷ÁöÇÏ´Â RNA·Î½á NGS library Á¦ÀÛ Àü, cDNA¿¡¼ rRNA¸¦ Á¦°Å (depletion)ÇÔÀ¸·Î½á Á¦ÀÛºñ¿ëÀ» Àý°¨ÇÒ ¼ö ÀÖ°í, mapping statistics ¶ÇÇÑ Çâ»ó½Ãų ¼ö ÀÖ´Ù. ÇÏÁö¸¸ ¸Å¿ì ±Ø¼Ò·®ÀÇ Total RNA¸¦ ÀÌ¿ëÇÒ °æ¿ì, ÀϹÝÀûÀÎ rRNA depletion ¹æ¹ýÀ» Àû¿ëÇϸé NGS library¸¦ Á¦Á¶ÇÏ´Â µ¥ ÇÊ¿äÇÑ RNA ¾ç¿¡ ¹ÌÄ¡Áö ¸ø ÇÏ°Ô µÈ´Ù.
±×¸² 1. Schematic of technology in the SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian
±×¸² 2. SMARTer¢ç Stranded Total RNA-Seq Kit v2 - Pico Input MammalianÀ» ÀÌ¿ëÇÏ¿© Á¦ÀÛÇÑ NGS LibraryÀÇ ±¸Á¶
The adapters added using the SMARTer¢ç RNA Unique Dual Index Kit - 24U (Code 634451) contain sequences allowing clustering on any Illumina flow cell (P7 shown in light blue, P5 shown in red, Index 1 [i7] sequence shown in orange, and Index 2 [i5] sequence shown in yellow), as well as the regions recognized by sequencing primers Read Primer 2 (Read 2, purple) and Read Primer 1 (Read 1, green). Read 1 generates sequences antisense to the original RNA, while Read 2 yields sequences sense to the original RNA (orientation of original RNA denoted by 5' and 3' in dark blue). The first 8 nt of the second sequencing read (Read 2) are UMIs (dark purple) followed by 3 nucleotides derived from the Pico v3 SMART UMI Adapter (shown as XXX).
Applications
- Robust NGS library construction that retains strand information
- RNA-seq on all Illumina platforms
- Coding and noncoding information from total mammalian RNA of any quality, including RNA obtained from FFPE samples
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
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Keyword : pico v3, BioMarker,FFPE,Cell-free RNA,Degraded RNA,Non-coding RNA,ncRNA,lncRNA,Cancer, Transcriptomics,transcriptome,LCM,stranded-seq,smarter,random primer,smart-seq,picov3
Stranded RNA-seq
´ÜÀϼ¼Æ÷ (single cell)ÀÇ stranded RNA-Seq li..
Single cell Stranded Kit
UDI, UMI Àû¿ëÀ¸·Î ´õ¿í Ưº°ÇØÁø
[NEW] Pico Input Mammalian (v3)
Picogram RNA·ÎºÎÅÍ Strand-specific illumina ..
Pico Input Mammalian (v2)
Low Input Mammalian
High Input Strand- Specific Total RNA-Seq
HI Mammalian
Stranded RNA-Seq Kit
Total RNA-Seq Kit (FFPE, LCM »ùÇà ºÐ¼®)