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PrimeScript¢â II High Fidelity One Step RT-PCR Kit (Code R026A)
1. |
PrimeScript II RT Enzyme Mix |
50 §¡ |
2. |
PrimeSTAR GXL for 1 step RT-PCR |
200 §¡ |
3. |
2¡¿One Step High Fidelity Buffer |
625 §¡¡¿2 |
4. |
Control F-1 primer*1 (20 ¥ìM) |
10 §¡ |
5. |
Control R-1 primer*2 (20 ¥ìM) |
10 §¡ |
6. |
Positive Control RNA (2¡¿105 copies/§¡) |
20 §¡ |
7. |
RNase Free dH2O |
625 §¡¡¿2 |
*1 Positive Control RNA (upstream sense primer)
*2 Positive Control RNA (downstream anti-sense primer)
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RNA template |
ÀÏ¹Ý |
ÁõÆø »çÀÌÁî |
8 kb ÁõÆø È®ÀÎ |
¿ªÀü»ç È¿¼Ò |
PrimeScript¢ç II RTase (45¡ÆC¿¡¼ »ç¿ë) |
DNA polymerase |
1 step RT-PCRÀ» À§ÇÑ PrimeSTAR GXL¢ç |
RNase inhibitor |
Æ÷ÇÔ (PrimeScript II RT Enzyme Mix¿¡ Æ÷ÇÔµÊ) |
1st strand cDNA
ÇÕ¼º¿ë ÇÁ¶óÀÌ¸Ó |
Gene specific downstream primer (PCR¿ë antisense primer)
ÁÖÀÇ: Random primer ¶Ç´Â Oligo dT primer´Â »ç¿ëÇÒ ¼ö ¾øÀ½ |
Á¶ÀÛ |
ÇϳªÀÇ Æ©ºê ³»¿¡¼ ¿¬¼ÓÀûÀ¸·Î RT-PCRÀ» ÁøÇà |
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- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â: Clontech PCR Thermal Cycler GP