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Home > ÀüÁ¦Ç°º¸±â > PCR > RT-PCR °ü·Ã Á¦Ç° > PrimeScript¢â II High Fidelity One Step RT-PCR Kit
°í¼Ó¹ÝÀÀ, ³ôÀº Á¤È®µµ ½ÇÇö - 1step RT-PCR kit

PrimeScript¢â II High Fidelity One Step RT-PCR Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R026A
PrimeScript¢â II High Fidelity One Step RT-PCR Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ new
50 ȸ
390,400¿ø 
488,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
R026A_e.v2408Da.pdf
Takara
R026B
PrimeScript¢â II High Fidelity One Step RT-PCR Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ new
200 ȸ (R026A x 4)
1,756,800¿ø 

  • ¿ªÀü»ç 10ºÐ, PCR ½ÅÀå ½Ã°£ 10ÃÊ/kbÀÇ °í¼Ó ¹ÝÀÀÀ» À§ÇÑ 1 step RT-PCR kit
  • High fidelity PCR È¿¼ÒÀÎ PrimeSTAR¢ç GXL ·Î Á¤È®ÇÑ ÁõÆø ½ÇÇö: GC rich, long target ÁõÆø¿¡µµ ÃÖÀû
  • CloningÀ» À§ÇÑ insertÀÇ RT-PCRÀ» 1 stepÀ¸·Î °£ÆíÇÏ°Ô ÁøÇà °¡´É
Á¦Ç°¼³¸í
º» Á¦Ç°Àº Á¤È®¼ºÀÌ ³ôÀº RT-PCRÀ» ´ÜÀÏ Æ©ºê ³»¿¡¼­ ¿¬¼ÓÀûÀ¸·Î ½Ç½ÃÇÏ´Â 1 step RT-PCR¿ë kit ÀÌ´Ù.
¿ªÀü»ç È¿¼Ò¿¡´Â ½ÅÀ强ÀÌ ¶Ù¾î³ª full-length cDNA ÇÕ¼º¿¡ ÃÖÀûÀÎ PrimeScript¢â II RTase¸¦, PCR¿¡´Â long fragment, GC rich ¼­¿­µµ Á¤È®ÇÏ°Ô ÁõÆøÇÏ´Â °í¼º´É high fidelity PCR È¿¼Ò PrimeSTAR¢ç GXL DNA Polymerase¸¦ »ç¿ëÇØ 1 step RT-PCR¿¡ ÃÖÀûÈ­ÇÏ¿´´Ù.
µû¶ó¼­, ±¤¹üÀ§ÇÑ ³óµµÀÇ RNA·ÎºÎÅÍ, ¿ªÀü»ç ¹ÝÀÀ 10ºÐ, PCR ½ÅÀå ½Ã°£ 10ÃÊ/kbÀÇ °í¼Ó Á¶°Ç¿¡¼­ ´Ù¾çÇÑ Å¸°ÙÀ» ½Å¼Ó, °£Æí, Á¤È®ÇÏ°Ô ÁõÆøÇÏ¿© cDNA ÁõÆø »ê¹°À» ¾òÀ» ¼ö ÀÖ´Ù.
³»¿ë (50 §¡ ¹ÝÀÀ½Ã 50ȸ ¹ÝÀÀºÐ)
PrimeScript¢â II High Fidelity One Step RT-PCR Kit (Code R026A)
1.

PrimeScript II RT Enzyme Mix

50 §¡

2.

PrimeSTAR GXL for 1 step RT-PCR

200 §¡

3.

2¡¿One Step High Fidelity Buffer

625 §¡¡¿2

4.

Control F-1 primer*1 (20 ¥ìM)

10 §¡

5.

Control R-1 primer*2 (20 ¥ìM)

10 §¡

6.

Positive Control RNA (2¡¿105 copies/§¡)

20 §¡

7.

RNase Free dH2O

625 §¡¡¿2


*1 Positive Control RNA (upstream sense primer)
*2 Positive Control RNA (downstream anti-sense primer)
º¸Á¸ - 20¡É
Ư¡

RNA template

ÀϹÝ

ÁõÆø »çÀÌÁî

8 kb ÁõÆø È®ÀÎ

¿ªÀü»ç È¿¼Ò

PrimeScript¢ç II RTase (45¡ÆC¿¡¼­ »ç¿ë)

DNA polymerase

1 step RT-PCRÀ» À§ÇÑ PrimeSTAR GXL¢ç

RNase inhibitor

Æ÷ÇÔ (PrimeScript II RT Enzyme Mix¿¡ Æ÷ÇÔµÊ)

1st strand cDNA
ÇÕ¼º¿ë ÇÁ¶óÀ̸Ó

Gene specific downstream primer (PCR¿ë antisense primer)
ÁÖÀÇ: Random primer ¶Ç´Â Oligo dT primer´Â »ç¿ëÇÒ ¼ö ¾øÀ½

Á¶ÀÛ

ÇϳªÀÇ Æ©ºê ³»¿¡¼­ ¿¬¼ÓÀûÀ¸·Î RT-PCRÀ» ÁøÇà

°ü·ÃÁ¦Ç°
- gDNA Á¦°Å Ä÷³À¸·Î °í¼øµµ RNA Á¤Á¦: TaKaRa MiniBEST Universal RNA Extraction Kit
- 16¡É, 30ºÐ ¿Ï·á, ³ôÀº È¿À²°ú Æí¸®¼ºÀ» °âºñÇÑ 2 x ligation premix: DNA Ligation Kit £¼Mighty Mix£¾
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â: Clontech PCR Thermal Cycler GP

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