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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Single cell DNA-Seq > PicoPLEX¢ç Single Cell DNA-Seq Kit
Single-cell NGS library prep for SNV and CNV detection

PicoPLEX¢ç Single Cell DNA-Seq Kit

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(ºÎ°¡¼¼º°µµ)
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Clontech
R300669
PicoPLEX¢ç Gold Single Cell DNA-seq Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24ȸ
2,079,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x102187
Clontech
R300670
PicoPLEX¢ç Gold Single Cell DNA-seq Kit
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96ȸ
5,176,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
R300698
PicoPLEX¢ç Gold Single Cell DNA-seq Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
384ȸ (R300670 x 4)
18,634,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

* º» Á¦Ç°Àº ±âÁ¸ PicoPLEX¢ç DNA-Seq Kit (Code R300381)ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°À̸ç, indexing primer kit°¡ Æ÷ÇԵǾîÀÖÁö ¾Ê½À´Ï´Ù.

  • ¸Å¿ì ³ôÀº ÀçÇö¼º°ú Á¤È®¼ºÀ¸·Î CNV, SNV, aneuploidy (¿°»öü À̼ö¼º) °ËÃâ
  • Single cell SNV °ËÃâ¿¡ ³Î¸® »ç¿ëµÇ°í ÀÖ´Â MDA ±â¼ú º¸´Ù °í¼º´É
  • Hands-on time 30ºÐÀÇ È¿À²ÀûÀÎ ÇÁ·ÎÅäÄÝ (Illumina¢ç NGS library Á¦À۽ð£: ~ 3½Ã°£)
  • Highly uniform coverage with low allele drop-in and drop-out rate
Á¦Ç°¼³¸í
PicoPLEX¢ç Gold Single Cell DNA-Seq Kit (ÀÌÇÏ, PicoPLEX Gold kit) Àº Single cell·ÎºÎÅÍ °íÇ°ÁúÀÇ Illumina¢ç DNA library¸¦ Á¦ÀÛÇÏ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°¿¡ Àû¿ëµÈ PicoPLEX¢ç Whole Genome Amplification (WGA) ±â¼úÀº Á¤È®¼º°ú ÀçÇö¼º ³ôÀº copy number variants (CNVs), single nucleotide variants (SNVs), indels, small structural variants¸¦ °ËÃâ °¡´ÉÇÏ´Ù. ƯÈ÷ »õ·ÎÀÌ ¾÷±×·¹À̵åµÈ º» Á¦Ç°, PicoPLEX Gold KitÀº SNV °ËÃâ·Î Àß ¾Ë·ÁÁø Multiple Displacement Amplification (MDA) ±â¼úÀ» »óȸÇÏ´Â ¼º´ÉÀ» ³ªÅ¸³½´Ù (Highly reproducible and accurate single cell whole-genome amplification using next-generation PicoPLEX technology).
PicoPLEX Gold Kit¿¡ Àû¿ëµÈ PicoPLEX WGA quasi-random priming ±â¼úÀº fixed ¶Ç´Â unfixed single cellÀÇ CNV °ËÃâÀ» À§ÇÑ Whole Genome Amplification (WGA)ÀÇ Àü ¼¼°èÀûÀÎ Gold Standard ±â¼ú·Î ¾Ë·ÁÁ® ÀÖ´Ù. PicoPLEX ±â¼úÀº º»µð ¿°»öü À̼ö¼º (aneuploidies)°ú CNV °ËÃâ¿ëÀ¸·Î °³¹ßµÇ¾úÁö¸¸, È¿¼Ò, Primer, ÇÁ·ÎÅäÄÝ µîÀÇ °³·® ¹× ÃÖÀûÈ­¸¦ ÅëÇÏ¿© CNV, SNV, indel, small structural variant °ËÃâ¿¡ ´ëÇÑ sequencing coverage, ±ÕÀϼº (uniformity), Á¤È®¼º (accuracy)À» ´ëÆø Çâ»ó½ÃÄ×´Ù.

º¸´Ù Çâ»óµÈ ¼º´ÉÀÇ PicoPLEX Gold KitÀº ´Ü¼øÇÑ 4¹øÀÇ ½ÇÇè ½ºÅÜ°ú ÃÖ¼ÒÇÑÀÇ hands-on timeÀ¸·Î fixed ¶Ç´Â unfixed single cell¿¡¼­ 3½Ã°£ ³»¿¡ Illumina¢ç NGS library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç° ³»¿¡´Â Illumina¢ç Index´Â Æ÷ÇԵǾîÀÖÁö ¾ÊÀ¸¸ç, ´ÙÄ«¶ó¹ÙÀÌ¿À¿¡¼­ Á¦°øÇÏ´Â ¸ðµç DNA Index Kits¸¦ Àû¿ë °¡´ÉÇÏ´Ù ( DNA Index Kit ¹Ù·Î°¡±â)


±×¸² 1. PicoPLEX Gold Single Cell DNA-Seq technology. Panel A. Schematic depicting the simple, four-step PicoPLEX Gold workflow with minimum hands-on time. Panel B. Schematic illustrating the PicoPLEX Gold chemistry. Cellular gDNA extracted in Step1 is used as the template for multiple cycles of quasi-random priming and linear amplification followed by exponential library amplification.


±×¸² 2. Real-time analysis of library amplification using the PicoPLEX Gold Kit. A typical real-time amplification analysis of libraries prepared with PicoPLEX Gold Kit using triplicates of single (blue) and five (purple) GM12878 cells, relative to the NTC (gray). Results were obtained using the CFX96 Touch Real-Time PCR Detection System with EvaGreen and fluorescein as the dyes.
Comparison data to Multiple Displacement Amplification (MDA)
[Technical Note] Highly reproducible and accurate single cell whole-genome amplification using next-generation PicoPLEX technology


±×¸² 3. Key features of PicoPLEX Gold: comprehensive coverage, high uniformity, and reproducibility (in comparison to QIASeq FX). Panel A. A log-log plot showing the number of bases covered at various depths of sequencing (~30M read pairs, PE 2 x 150bp). The coverage of PicoPLEX Gold was similar to QIASeq FX (MDA) at lower depths and greater at higher depths. Panel B. Examples of the coverage patterns of PicoPLEX Gold and QIASeq FX in gDNA (NA12878) and single-cell samples (GM12878) for a 75-kb window (chr2). MDA has a higher propensity to leave large gaps in the genome, whereas PicoPLEX Gold has a more uniform coverage. Panel C. The reproducibility of coverage evaluated by comparing total reads in 100-kb bins. The consistency of the total reads in each window from the two single-cell libraries is significantly higher for PicoPLEX Gold (left), both in comparison to QIASeq FX (right) and to other technologies in the market (data now shown).


±×¸² 4. High-quality single nucleotide variant (SNV) detection with PicoPLEX Gold. Panel A. Comparison of SNV-detection rate between QIASeq FX (MDA), PicoPLEX Gold (PP Gold), and PicoPLEX WGA (PP WGA) kits from one cell (1c), five cells (5c) or 15 pg of NA12878 gDNA inputs. Single and five cells were sequenced to a depth of ~35M read pairs, and gDNA samples to a depth of ~40M read pairs. The high fidelity and robust coverage of PicoPLEX Gold (blue bars) provide a clear advantage in detecting a greater (~2-9 fold) number of high-quality SNVs compared to QIASeq FX (gray bars) and PicoPLEX WGA (purple bar). Panel B. The symmetric distribution of the B-allele frequencies for PicoPLEX Gold (blue bars), centered around 0.5, indicating a balanced recovery of both alleles. PicoPLEX has better allele balance compared to QIASeq FX (MDA) (gray bars). Panel C. Unbiased amplification of PicoPLEX Gold results in the lowest allele drop-out (false-negative) rates among all single cell library-preparation technologies tested. Panel D. High fidelity of the polymerase used in PicoPLEX Gold Kit (blue bars) leads to minimal allele drop-in rates that are comparable to QIASeq FX (gray bars) and significantly lower than PicoPLEX WGA (purple bar).
Applications
Library preparation for the following cell types:
  • Cancer research: circulating tumor cells, cells, or DNA from laser capture microdissection (LCM), and cells from fine needle aspirate (FNA)
  • Reproductive health research: embryonic cells and circulating fetal cells
  • Developmental biology: embryonic cells, stem cells, and rare cells
  • Metagenomics and microbiology: microbial cells, microbiome and environmental samples
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
- Cell Extraction Buffer
- Cell Extraction Enzyme
- PreAmp Buffer
- PreAmp Enzyme
- Amplification Buffer
- Amplification Enzyme
- Nuclease-Free Water

Keyword : Sngle cell,single cell analysis,single cell NGS,WGA,Whole genome amplification,NGS,PGS,PGD,PGA,žƻêÀü°Ë»ç,»êÀü°Ë»ç,PGTA,CNV,SNV,SNP,Genotyping,variation,PicoPLEX DNA-Seq Kit,R300381

Single cell DNA-Seq
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Single-cell NGS library prep for SNV and CNV..
PicoPLEX¢ç Single Cell DNA-Seq Kit