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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Stranded RNA-seq > [New]Pico input stranded RNA-Seq Kit
FFPE, LCM, cell-free RNAµîÀÇ ±Ø¼Ò·® low quality RNA¿¡¼­ ÀçÇö¼º ³ôÀº °á°ú

[New]Pico input stranded RNA-Seq Kit

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Clontech
634357
SMART-Seq¢ç Total RNA Pico Input (ZapR¢â Mammalian)
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24 ȸ (Package)
3,226,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x348194
Clontech
634358
SMART-Seq¢ç Total RNA Pico Input (ZapR¢â Mammalian)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96 ȸ (Package)
7,967,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
634359
SMART-Seq¢ç Total RNA Pico Input (ZapR¢â Mammalian)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
384 ȸ (634355x4)
28,682,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

¡Ø º¯°æ»çÇ×
º» Á¦Ç°Àº ±âÁ¸ SMARTer¢ç Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian (Code 634411, 634412, 634413, 634414)ÀÇ ¸®´º¾ó Á¦Ç°ÀÔ´Ï´Ù. ÀϺΠ±¸¼ºÇ°ÀÇ ¾÷±×·¹À̵å·Î ¾Æ·¡¿Í °°ÀÌ ¼º´ÉÀÌ °³¼±µÇ¾ú½À´Ï´Ù.
i. rRNA Á¦°Å ½Ã¾àÀÎ ZapR¢âÀÇ ¼º´ÉÀÌ Çâ»óµÇ¾î ¿øÄ¡ ¾Ê´Â read¸¦ ´õ¿í È¿°úÀûÀ¸·Î Á¦°ÅÇÕ´Ï´Ù.
ii. ÇÊ¿äÇÑ index¸¦ ¼±ÅÃÇÏ¿© Àû¿ëÇÒ ¼ö ÀÖµµ·Ï Á¦Ç°±¸¼º¿¡¼­ index¸¦ Á¦¿ÜÇÏ¿´½À´Ï´Ù. ÇÊ¿ä½Ã º°µµ ±¸¸Å ¹Ù¶ø´Ï´Ù.
>> ȣȯµÇ´Â Unique Dual Index kits (UDI kits)
ÀÚ¼¼ÇÑ ³»¿ëÀº ´ÙÄ«¶óÄÚ¸®¾Æ (02-2081-2510)·Î ¹®ÀÇ ÁÖ¼¼¿ä.

  • pg ¼öÁØ ±Ø¼Ò·® »ùÇà Àû¿ë - human, mouse, rat À¯·¡ÀÇ 250 pg - 100 ng total RNA
  • ´Ù¾çÇÑ »ùÇà Àû¿ë - FFPE, LCM, cell-free RNAµî ³·Àº quality RNA¿¡¼­µµ ÀçÇö¼º ³ôÀº °á°ú È®ÀÎ
  • °í¼º´É sequencing °¡´É - PhiX controlÀ» Ãß°¡ÇÏÁö ¾Ê¾Æµµ ³ôÀº ¼öÁØÀÇ cluster passing filter (%PF)¸¦ ¾òÀ» ¼ö ÀÖ°í, ÀûÀº ¹Ýº¹È½¼ö·Îµµ ´õ ¸¹Àº transcript¸¦ È®ÀÎ °¡´É
  • Strand Á¤º¸ º¸Á¸ - °¢ transcriptÀÇ strand Á¤º¸ (sense/antisense)¸¦ ³ôÀº Á¤È®µµ·Î È®ÀÎ °¡´É
  • ºü¸£°í °£ÆíÇÑ ÇÁ·ÎÅäÄÝ - Àü °úÁ¤ 6½Ã°£¸¸¿¡ ¿Ï·á, buffer Á¶¼º ¾÷±×·¹À̵å·Î library purification ½Ã°£ ´ÜÃà
  • Illumina¢ç Ç÷§Æû¿¡ ȣȯ - Illumina¢ç ºÐ¼®¿ë index¸¦ Àû¿ëÇÏ¿© ÃÖ´ë 384°³ pooling °¡´É
Á¦Ç°¼³¸í
SMART-Seq¢ç Total RNA Pico Input with (ZapR¢â Mammalian)Àº picogram input (250 pg - 10 ng total RNA)ÀÇ ´Ù¾çÇÑ RNA (high quality, partially degraded, low quality total RNA)¿¡¼­ È¿°úÀûÀ¸·Î Illumina¢ç NGS library¸¦ Á¦ÀÛÇϱâ À§ÇØ °³¹ßµÇ¾ú´Ù. ´ç»çÀÇ µ¶ÀÚÀûÀÎ SMART (Switching Mechanism at 5¡¯ end of RNA Template) ±â¼úÀ» »ç¿ëÇØ ±Ø¼Ò·®ÀÇ total RNA»Ó ¾Æ´Ï¶ó FFPE, LCM, cell-free RNA µî RNAÀÇ integrity°¡ ÇöÀúÈ÷ ³·Àº »ùÇÿ¡¼­µµ NGS library Á¦ÀÛÀÌ °¡´ÉÇÏ´Ù. ÀÌ Á¦Ç°Àº ºü¸£°í °£ÆíÇÑ ÇÁ·ÎÅäÄÝÀÌ Æ¯Â¡À¸·Î RT¿Í PCR ´Ü°è¿¡¼­ index¿Í adapter¸¦ Ãß°¡ÇØ strand Á¤º¸ (sense/antisense)¸¦ º¸À¯ÇÏ´Â library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. ÃÑ 6½Ã°£¸¸¿¡ library prepºÎÅÍ purification±îÁö Àü °úÁ¤À» ÁøÇàÇÑ´Ù.

Total RNA¸¦ »ç¿ëÇÏ¿© NGS¸¦ ÁøÇàÇϸé 90% ÀÌ»óÀ» Ribosomal RNA (rRNA)°¡ Â÷ÁöÇϹǷΠlibrary prep Àü¿¡ À̸¦ Á¦°ÅÇØ¾ß sequencing ºñ¿ëÀ» ³·Ãß°í ´õ ¸¹Àºmapping Á¤º¸¸¦ ¾òÀ» ¼ö ÀÖ´Ù. ±×·¯³ª ±Ø¼Ò·®ÀÇ total RNA »ùÇÃÀº rRNA Á¦°Å °úÁ¤¿¡¼­ »ùÇÃÀÇ ¼Õ½Ç·Î ÀÎÇØ high-quality library »ý»êÀÌ ºÒ°¡´ÉÇØÁö´Â °æ¿ì°¡ ÀÖ´Ù. ÀÌ Á¦Ç°¿¡¼­´Â mammalian rRNA¿Í ÀϺΠmitochondrial RNA ¼­¿­¿¡ ƯÀÌÀûÀÎ probe·Î ribosomal cDNA (rRNA ºÐÀÚ¸¦ ÁÖÇüÀ¸·Î ÇÕ¼ºµÈ cDNA fragment)¸¦ Á¦°ÅÇØ ÀÌ·¯ÇÑ ¹®Á¦Á¡À» ±Øº¹ÇÏ¿´´Ù (ZapR¢â Mammalian).

ÀÌ Á¦Ç°Àº two channel SBS ±â¼úÀ» Àû¿ëÇÑ Illumina¢ç NextSeq, MiniSeq, HiSeq 3000/4000À» ÀÌ¿ëÇÏ´Â °æ¿ì¿¡µµ PhiX Control Spike-in RNAÀÇ Ã·°¡ ¾øÀÌ ³ôÀº ºñÀ²·Î Cluster passing filter (%PF)¸¦ ¾òÀ» ¼ö ÀÖ´Ù. 1ȸ running´ç, º¸´Ù »ý¹°ÇÐÀûÀ¸·Î ÀǹÌÀÖ´Â sequencing reads¸¦ ¸¹ÀÌ ¾òÀ» ¼ö ÀÖÀ¸¹Ç·Î ÀüüÀûÀ¸·Î sequencing ºñ¿ëÀ» ÁÙÀÏ ¼ö ÀÖ´Ù. ¶ÇÇÑ º» Á¦Ç°Àº ±âÁ¸ Á¦Ç°¿¡¼­ ¾÷±×·¹À̵åµÈ PCR Buffer¸¦ ÀÌ¿ëÇϹǷΠº¸´Ù ³ôÀº NGS library Á¤Á¦ È¿À²À» ±â´ëÇÒ ¼ö ÀÖ´Ù.

*±× ¿Ü »ùÇà input¾ç¿¡ µû¸¥ Ãßõ Á¦Ç°:
- 10 ng - 100 ng total RNA (rRNA Á¦°Å ¹ÌÆ÷ÇÔ) : SMART-Seq¢ç Total RNA Mid Input (Code 635049)
- Mammalian total RNA 100 ng-1 ¥ìg : SMART-Seq¢ç Total RNA High Input (RiboGone¢â Mammalian) (Code 635046)


±×¸² 1. SMART-Seq¢ç Total RNA Pico Input (ZapR¢â Mammalian) ÇÁ·Î¼¼½º.
Àû¿ë
  • Strand Á¤º¸¸¦ °¡Áø NGS library Á¦ÀÛ
  • Illumina¢ç Ç÷§Æû RNA-seq
  • ´Ù¾çÇÑ quality¿¡ total mammalian RNA¿¡¼­ coding ¹× non-coding Á¤º¸ È®ÀÎ
    (FFPE »ùÇÿ¡¼­ Á¤Á¦ÇÑ RNA »ç¿ë °¡´É)
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
RNA-Seq ·¯´×¼¾ÅÍ¿¡¼­ ´õ ¸¹Àº µ¥ÀÌÅÍ¿Í FAQ¸¦ È®ÀÎÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.

Keyword :

Stranded RNA-seq
>
(with UMI) FFPE, LCM, cell-free RNAµîÀÇ ±Ø¼Ò..
[New](+UMI) Pico input stranded RNA-Seq Kit
>
FFPE, LCM, cell-free RNAµîÀÇ ±Ø¼Ò·® low qual..
[New]Pico input stranded RNA-Seq Kit
>
´ÜÀϼ¼Æ÷ (Single cell)¿¡¼­ stranded RNA-Seq ..
[New]Single cell stranded RNA-Seq Kit
>
Mid Input Strand- Specific Total RNA-Seq
[New]Mid Input Stranded RNA-Seq Kit
>
High Input Strand- Specific Total RNA-Seq
[New]High Input Stranded RNA-Seq Kit
>
UDI, UMI Àû¿ëÀ¸·Î ´õ¿í Ưº°ÇØÁø
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Input Mammalian(v3)
>
Picogram RNA·ÎºÎÅÍ Strand-specific illumina ..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Input Mammalian(v2)
>
´ÜÀϼ¼Æ÷ (single cell)ÀÇ stranded RNA-Seq li..
Single cell Stranded RNA-Seq Kit
>
High Input Strand- Specific Total RNA-Seq
High Input Stranded RNA-Seq Kit