- ³ôÀº Á¤È®µµ: 99% ÀÌ»óÀÇ Á¤È®µµ·Î °¢ Àü»çüÀÇ origin °¡´Ú ½Äº° °¡´É
- ³ôÀº °¨µµ: 100 pgÀÇ ÀûÀº RNA input¿¡ ÀÖ´Â ¼Ò·®ÀÇ Àü»çüµµ °ËÃâÀÌ °¡´É. rRNA¸¦ Á¦°ÅÇÑ RNA ¶Ç´Â poly(A)-purified RNA¸¦ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÔ.
- Illumina-ready library: PCR ÁõÆø ´Ü°è¿¡¼ Illumina index¿Í adaptor¸¦ Ãß°¡
- °¡Àå °£´ÜÇÑ ¿öÅ©Ç÷οì Á¦°ø: ´Ü 4½Ã°£ ¸¸¿¡ °£´ÜÇÏ°Ô library Á¦ÀÛ
Á¦Ç° ¼³¸í
º» Á¦Ç°Àº 99% ÀÌ»óÀÇ Á¤È®µµ·Î strand ¹æÇ⼺ Á¤º¸¸¦ À¯ÁöÇÏ´Â Illumina-ready library¸¦ ÁغñÇϱâ À§ÇÑ ¼Ö·ç¼ÇÀ» Á¦°øÇϸç, rRNA-depleted sample ¶Ç´Â poly(A)-enriched sample¿¡ ÃßõÇÑ´Ù. ¶ÇÇÑ eukaryotic ¹× prokaryotic RNA »ùÇÿ¡ ȣȯµÇ´Â random-priming ¹æ¹ýÀ» »ç¿ëÇϸç, 100 pg - 100 ng RNA inputÀ¸·Î °íÇ°Áú µ¥ÀÌÅ͸¦ »ý¼ºÇÑ´Ù.
ÀÌ Å°Æ®´Â coding, noncoding RNA Àüü¿¡ ´ëÇÑ Àü»çü Á¤º¸¸¦ Á¦°øÇϸç, ¿öÅ©ÇÃ·Î¿ì ¾È¿¡ Illumina index ¹× adapter¸¦ Ãß°¡ÇÏ´Â °úÁ¤ÀÌ Æ÷ÇÔµÇ¾î ¸Å¿ì °£ÆíÇÏ°Ô library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ Unique Dual Index Kits (Code 634752-634756)¸¦ »ç¿ëÇϸé ÃÖ´ë 384°³ÀÇ Illumina-ready library¸¦ multiplexingÇÒ ¼ö ÀÖ´Ù.
rRNA Á¦°Å°¡ ÇÊ¿äÇÑ °æ¿ì, 10 - 100 ng total RNA input¿¡ Àû¿ë°¡´ÉÇÑ RiboGone¢â- Mammalian kit¸¦ ÃßõÇÑ´Ù.
*±âŸ input amount¿¡ µû¸¥ Ãßõ Å°Æ®:
- Mammalian total RNA 250 pg-10 ng : SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Code 634485 ¿Ü)
- Mammalian total RNA 100 ng-1 ¥ìg : SMART-Seq Total RNA High Input (RiboGone Mammalian) (Code 635046)
Àû¿ë
- Illumina Ç÷§ÆûÀ» ÀÌ¿ëÇÑ prokaryotic ¶Ç´Â mammalian »ùÇÃÀÇ RNA-Seq
- Strand ¹æÇ⼺ Á¤º¸¸¦ À¯ÁöÇÏ´Â NGS library Áغñ
- Coding ¹× noncoding ¼¿ Á¤º¸ ºÐ¼®
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
RNA-Seq ·¯´×¼¾Å͸¦ ÅëÇØ º» Á¦Ç°¿¡ ´ëÇÑ ´õ ¸¹Àº µ¥ÀÌÅÍ¿Í FAQ¸¦ Âü°íÇϼ¼¿ä.