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SNV, CNV 검출 가능한 Whole genome amplification (WGA) from single cells

PicoPLEX® Single Cell WGA Kit v3

제조사	제품코드	제품명	용량	가격 (부가세별도)
Clontech	R300718	PicoPLEX® Single Cell WGA Kit v3	24 회	가격문의
Clontech	R300722	PicoPLEX® Single Cell WGA Kit v3	96 회	가격문의
Clontech	R300723	PicoPLEX® Single Cell WGA Kit v3	480 회	가격문의

Product index

PGT-A
- Embgenix™ PGT-A Kit (..
SMARTer RNA-Seq 개요
mRNA-Seq (Single cell)
Stranded RNA-seq
Small RNA-Seq
- [Cancer Research] Canc..
- Small RNA-Seq Kit
Immune-Seq
기타 RNA-Seq
- Full length cDNA 합성
- Target RNA Capture for..
DNA-seq 개요
ChIP-Seq / Meth-Seq
Low Input DNA-Seq
High input DNA-seq
- ThruPLEX® DNA-Seq HV
- ThruPLEX® Tag-Seq HV
Single cell DNA-Seq
Whole Genome Amplification..
Index Kit
NGS Library 정량
- Library Quantification..
NGS Sample preparation
NGS 분석용 PCR 효소
NGS 관련 장비
[Legacy] NGS Products

* 본 제품은 기존 PicoPLEX^® Single Cell WGA Kit (Code R300671)의 업그레이드 버전 제품으로, CNV는 물론 SNV의 검출까지 가능한 최고 민감도 수준의 제품입니다.

Single cells 혹은 15 pg of DNA 수준에서 재현성 있는 결과
월등한 Allele representation
자동화 기기 적용에 최적
모든 실험 적용에서 편향성 없는 결과 (e.g., microarray and PCR)

제품설명

PicoPLEX Single Cell WGA Kit v3는 single-tube protocol를 이용하여, 1-10개의 세포 혹은 동등한 pg 수준의 gDNA로부터 재현성 있는 증폭이 가능하다. Cell lysis, whole genome preamplification, 증폭 과정이 2.5시간 내에 진행되며, 최소화된 에러발생률로 샘플을 2 μg 수준으로 증폭한다. 또한 High-fidelity 효소를 도입함으로써, 기존 제품에서 가능한 CNV 뿐 아니라, SNV 검출에도 활용될 수 있다.

샘플

- Mammalian cells (1-10 cells)
- Bacterial cells (1,000-10,000 cells)
- Isolated DNA (15-60 pg of human DNA)
- Sorted chromosomes
- Intact or fragmented, single- or double-stranded DNA

적용

- Copy number variation (CNV) analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
- Target enrichment or amplicon enrichment for mutation detection
- SNP genotyping

그림 1. PicoPLEX^® WGA기술의 원리
Cells are lysed, quasi-random primers pre-amplify the DNA selectively, and a final PCR amplification adds the Illumina barcodes. ( PicoPLEX^® 기술 개요 보기)

그림 2. PicoPLEX WGA v3 workflow overview
Steps involved in PicoPLEX Single Cell WGA Kit v3 sample preparation starting from a single cell.

자세한 구성은 CoA를 참고하세요.

Citation

PicoPLEX^® WGA Kit는 PicoPLEX^® Single Cell WGA Kit로 업데이트되었으며, 가장 최신버전은 PicoPLEX^® Single Cell WGA Kit v3입니다. Citation이 동일합니다.

Validation Studies for Single Circulating Trophoblast Genetic Testing as a Form of Noninvasive Prenatal Diagnosis
American Journal of Human Genetics    |    2019 Dec 05    |    PubMed ID: 31785788    |    Read Article

Mutated clones driving leukemic transformation are already detectable at the single-cell level in CD34-positive cells in the chronic phase of primary myelofibrosis
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Investigation of somatic CNVs in brains of synucleinopathy cases using targeted SNCA analysis and single cell sequencing
Acta Neuropathologica Communications    |    2019 Dec 23    |    PubMed ID: 31870437    |    Read Article

Preimplantation genetic testing for aneuploidy: a comparison of live birth rates in patients with recurrent pregnancy loss due to embryonic aneuploidy or recurrent implantation failure
Human Reproduction    |    2019 Dec 01    |    PubMed ID: 31811307    |    Read Article

Binding of a C-type lectin’s coiled-coil domain to the Domeless receptor directly activates the JAK/STAT pathway in the shrimp immune response to bacterial infection
PLoS Pathogens    |    2017 Sep 20    |    PubMed ID: 28931061    |    Read Article

SlZHD17 is involved in the control of chlorophyll and carotenoid metabolism in tomato fruit
Horticulture Research    |    2021 Dec 01    |    PubMed ID: 34848692    |    Read Article

Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single-cell level, et al. Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single-cell level
Cancer Science    |    2021 Apr 02    |    PubMed ID: 33484069    |    Read Article

Prognostic impact of circulating tumor cells detected with the microfluidic “universal CTC-chip” for primary lung cancer, et al. Prognostic impact of circulating tumor cells detected with the microfluidic “universal CTC-chip” for primary lung cancer
Cancer Science    |    2022 Jan 28    |    PubMed ID: 34964211    |    Read Article

Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation
Frontiers in Cell and Developmental Biolo…    |    2021 Dec 03    |    PubMed ID: 34926439    |    Read Article

Single-Circulating Tumor Cell Whole Genome Amplification to Unravel Cancer Heterogeneity and Actionable Biomarkers
International Journal of Molecular Scienc…    |    2022 Jul 29    |    PubMed ID: 35955517    |    Read Article

Isolation of TTF-1 Positive Circulating Tumor Cells for Single-Cell Sequencing by Using an Automatic Platform Based on Microfluidic Devices
International Journal of Molecular Scienc…    |    2022 Dec 01    |    PubMed ID: 36499466    |    Read Article

Genomic Analysis of Hematopoietic Stem Cell at the Single-Cell Level: Optimization of Cell Fixation and Whole Genome Amplification (WGA) Protocol
International Journal of Molecular Scienc…    |    2020 Oct 06    |    PubMed ID: 33036143    |    Read Article

Functional Analysis of the Active Site of a Metallo-β-Lactamase Proliferating in Japan
Antimicrobial Agents and Chemotherapy    |    1999 Jul 06    |    PubMed ID: 10952572    |    Read Article

A Rapid Method for Label-Free Enrichment of Rare Trophoblast Cells from Cervical Samples
Scientific Reports    |    2019 Aug 20    |    PubMed ID: 31431640    |    Read Article

Detection of circulating sarcoma tumor cells using a microfluidic chip-type cell sorter
Scientific Reports    |    2019 Dec 27    |    PubMed ID: 31882696    |    Read Article

Kinship analysis on single cells after whole genome amplification
Scientific Reports    |    2020 Sep 04    |    PubMed ID: 32887915    |    Read Article

First detection of TBE virus in ticks and sero-reactivity in goats in a non-endemic region in the southern part of Switzerland (Canton of Ticino).
Ticks and tick-borne diseases    |    2019 Aug 15    |    PubMed ID: 31047827    |    Read Article

Serological Investigation and Genetic Characteristics of Pseudorabies Virus in Hunan Province of China From 2016 to 2020
Frontiers in Veterinary Science    |    2021 Dec 16    |    Read Article

A common variant of CNTNAP2 is associated with sub-threshold autistic traits and intellectual disability
PLoS ONE    |    2021 Dec 13    |    PubMed ID: 34898614    |    Read Article

Use of amplicon-based sequencing for testing fetal identity and monogenic traits with Single Circulating Trophoblast (SCT) as one form of cell-based NIPT
PLoS ONE    |    2021 Apr 15    |    PubMed ID: 33857205    |    Read Article

Haplotyping by linked-read sequencing (HLRS) of the genetic disease carriers for preimplantation genetic testing without a proband or relatives
BMC Medical Genomics    |    2020 Aug 20    |    PubMed ID: 32819358    |    Read Article

A novel method for noninvasive diagnosis of monogenic diseases from circulating fetal cells.
Prenatal diagnosis    |    2021 Dec 10    |    PubMed ID: 32673403    |    Read Article

Cell-based non-invasive prenatal diagnosis in a pregnancy at risk of cystic fibrosis.
Prenatal diagnosis    |    2022 Jan 03    |    PubMed ID: 33150588    |    Read Article

Reliable detection of subchromosomal deletions and duplications using cell-based noninvasive prenatal testing
Prenatal Diagnosis    |    2018 Nov 19    |    PubMed ID: 30357877    |    Read Article

Utility of Plasmodium falciparum DNA from rapid diagnostic test kits for molecular analysis and whole genome amplification
Malaria Journal    |    2020 May 27    |    PubMed ID: 32460780    |    Read Article

A Silicon-based Coral-like Nanostructured Microfluidics to Isolate Rare Cells in Human Circulation: Validation by SK-BR-3 Cancer Cell Line and Its Utility in Circulating Fetal Nucleated Red Blood Cells
Micromachines    |    2019 Feb 17    |    PubMed ID: 30781548    |    Read Article

Use of amplicon-based sequencing for testing fetal identity and monogenic traits with single circulating trophoblast (SCT) prenatal diagnosis
medRxiv    |    2020 Jun 02    |    Read Article

Intercellular extrachromosomal DNA copy number heterogeneity drives cancer cell state diversity
bioRxiv    |    2023 Jan 21    |    Read Article

Artificial intelligence-driven morphology-based enrichment of malignant cells from body fluid
bioRxiv    |    2023 Jan 25    |    Read Article

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Keyword : Sngle cell,단일세포,싱글셀,single cell analysis,single cell NGS,Ultra low input,WGA,Whole genome amplification,NGS,PGS,PGD,PGA,태아산전검사,산전검사,DNA-seq,CNV,SNV,SNP,Genotyping,array,variation,DNA variation

Whole Genome Amplification (WGA)

[기술개요] ThruPLEX & PicoPLEX

[Cancer Research] HLA typing

[논문] PicoPLEX® 기술과 다양한 applications

SNV, CNV 검출 가능한 Whole genome amplificat..

PicoPLEX® Single Cell WGA Kit v3

Whole genome amplification (WGA) from single..

PicoPLEX® Single Cell WGA Kit