¿À´Ã ÇÏ·ç ±×¸¸º¸±â
´Ý±â
·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
|
Á¦Ç°»ó´ã¹®ÀÇ
°í°´Áö¿ø
¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
02-2081-2510
Email ¦¢
support@takara.co.kr
´ëÀüÁö»ç
¾÷¹«¾È³»¦¢´ëÀü/ÃæÃ»Áö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
042-828-6525
Email ¦¢
tkbd@takara.co.kr
¾÷¹«½Ã°£¾È³»
[ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Products
°øÁö»çÇ×
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
樨毢
ȸ»ç¼Ò°³
That's Good Science!
Á¦Ç°¼±Åð¡À̵å
ÀüÁ¦Ç°º¸±â
Learning Center
Custom Service
À̺¥Æ®/Ä·ÆäÀÎ
´ÙÄ«¶ó¼Ò½Ä
´ÙÄ«¶ó°ø½Ã
½ÅÁ¦Ç°Á¤º¸
´ÙÄ«¶ó ¶óÀ̼±½º
±â¼úÁö¿ø¹®ÀÇ
Á¤º¸ÀÚ·á½Åû
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
LS&B
ºê·Î¼Å
eNews
CoA & MSDS
¿Â¶óÀÎÅø
樨毢
¿Â¶óÀμ¼¹Ì³ª
ȸ»ç¼Ò°³
µµÀԺ귣µå ¾È³»
´ë¸®Á¡¾È³»
ã¾Æ¿À½Ã´Â±æ
Welcome
About Thats Good Science
Watch the Videos - S3
Watch the Videos - S2
Watch the Videos - S1
Home
>
ÀüÁ¦Ç°º¸±â
>
NGS °ü·Ã
>
Whole Genome Amplification (WGA)
> PicoPLEX¢ç Single Cell WGA Kit v3
SNV, CNV °ËÃâ °¡´ÉÇÑ Whole genome amplification (WGA) from single cells
PicoPLEX¢ç Single Cell WGA Kit v3
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Clontech
R300718
PicoPLEX¢ç Single Cell WGA Kit v3
24 ȸ
°¡°Ý¹®ÀÇ
11.01 ~ 12.29
Clontech
R300722
PicoPLEX¢ç Single Cell WGA Kit v3
96 ȸ
°¡°Ý¹®ÀÇ
11.01 ~ 12.29
Clontech
R300723
PicoPLEX¢ç Single Cell WGA Kit v3
480 ȸ
°¡°Ý¹®ÀÇ
11.01 ~ 12.29
Product index
PGT-A
Embgenix¢â PGT-A Kit (..
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸..
mRNA-Seq (Single cell)
Single cell RNA-seq¿¡ ..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
´Ù·®ÀÇ ±Ø¼Ò·® »ùÇÃÀ» ..
±Ø¼Ò·® mRNAÀÇ 3¡¯ ¸»´Ü..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
´Ù·®ÀÇ ±Ø¼Ò·® »ùÇÃÀ» ..
Single cell RNA-seq¿¡ ..
Stranded RNA-seq
Single cell Stranded K..
[NEW] Pico Input Mamma..
Pico Input Mammalian (..
Low Input Mammalian
HI Mammalian
Stranded RNA-Seq Kit
Total RNA-Seq Kit (FFP..
Small RNA-Seq
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[Version 2] Human TCR ..
Human TCR a/b Profilin..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
SMART-Seq Human BCR (w..
SMART-Seq Human TCR (w..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
ChIP-Seq / Meth-Seq
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
Unique Dual Index Kits
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) SMARTer Ultra..
(Legacy) PicoPLEX¢ç DN..
(Legacy) ThruPLEX¢ç Pl..
(Legacy) ThruPLEX¢ç DN..
(Legacy) PicoPLEX¢ç WG..
* º» Á¦Ç°Àº ±âÁ¸ PicoPLEX
¢ç
Single Cell WGA Kit (Code R300671)ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°À¸·Î, CNV´Â ¹°·Ð SNVÀÇ °ËÃâ±îÁö °¡´ÉÇÑ ÃÖ°í ¹Î°¨µµ ¼öÁØÀÇ Á¦Ç°ÀÔ´Ï´Ù.
Single cells ȤÀº 15 pg of DNA ¼öÁØ¿¡¼ ÀçÇö¼º ÀÖ´Â °á°ú
¿ùµîÇÑ Allele representation
ÀÚµ¿È ±â±â Àû¿ë¿¡ ÃÖÀû
¸ðµç ½ÇÇè Àû¿ë¿¡¼ ÆíÇ⼺ ¾ø´Â °á°ú (e.g., microarray and PCR)
Á¦Ç°¼³¸í
PicoPLEX Single Cell WGA Kit v3´Â single-tube protocol¸¦ ÀÌ¿ëÇÏ¿©, 1-10°³ÀÇ ¼¼Æ÷ ȤÀº µ¿µîÇÑ pg ¼öÁØÀÇ gDNA·ÎºÎÅÍ ÀçÇö¼º ÀÖ´Â ÁõÆøÀÌ °¡´ÉÇÏ´Ù. Cell lysis, whole genome preamplification, ÁõÆø °úÁ¤ÀÌ 2.5½Ã°£ ³»¿¡ ÁøÇàµÇ¸ç, ÃÖ¼ÒÈµÈ ¿¡·¯¹ß»ý·ü·Î »ùÇÃÀ» 2 ¥ìg ¼öÁØÀ¸·Î ÁõÆøÇÑ´Ù. ¶ÇÇÑ High-fidelity È¿¼Ò¸¦ µµÀÔÇÔÀ¸·Î½á, ±âÁ¸ Á¦Ç°¿¡¼ °¡´ÉÇÑ CNV »Ó ¾Æ´Ï¶ó, SNV °ËÃâ¿¡µµ Ȱ¿ëµÉ ¼ö ÀÖ´Ù.
»ùÇÃ
- Mammalian cells (1-10 cells)
- Bacterial cells (1,000-10,000 cells)
- Isolated DNA (15-60 pg of human DNA)
- Sorted chromosomes
- Intact or fragmented, single- or double-stranded DNA
Àû¿ë
- Copy number variation (CNV) analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
- Target enrichment or amplicon enrichment for mutation detection
- SNP genotyping
±×¸² 1. PicoPLEX
¢ç
WGA±â¼úÀÇ ¿ø¸®
Cells are lysed, quasi-random primers pre-amplify the DNA selectively, and a final PCR amplification adds the Illumina barcodes. (
PicoPLEX
¢ç
±â¼ú °³¿ä º¸±â
)
±×¸² 2. PicoPLEX WGA v3 workflow overview
Steps involved in PicoPLEX Single Cell WGA Kit v3 sample preparation starting from a single cell.
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
Citation
PicoPLEX
®
WGA Kit
´Â
PicoPLEX
®
Single Cell WGA Kit
·Î ¾÷µ¥ÀÌÆ®µÇ¾úÀ¸¸ç, °¡Àå ÃֽŹöÀüÀº
PicoPLEX
®
Single Cell WGA Kit v3
ÀÔ´Ï´Ù. CitationÀÌ µ¿ÀÏÇÕ´Ï´Ù.
Validation Studies for Single Circulating Trophoblast Genetic Testing as a Form of Noninvasive Prenatal Diagnosis
American Journal of Human Genetics | 2019 Dec 05 | PubMed ID: 31785788 |
Read Article
Mutated clones driving leukemic transformation are already detectable at the single-cell level in CD34-positive cells in the chronic phase of primary myelofibrosis
NPJ Precision Oncology | 2021 Feb 04 | PubMed ID: 33542466 |
Read Article
Investigation of somatic CNVs in brains of synucleinopathy cases using targeted SNCA analysis and single cell sequencing
Acta Neuropathologica Communications | 2019 Dec 23 | PubMed ID: 31870437 |
Read Article
Preimplantation genetic testing for aneuploidy: a comparison of live birth rates in patients with recurrent pregnancy loss due to embryonic aneuploidy or recurrent implantation failure
Human Reproduction | 2019 Dec 01 | PubMed ID: 31811307 |
Read Article
Binding of a C-type lectin’s coiled-coil domain to the Domeless receptor directly activates the JAK/STAT pathway in the shrimp immune response to bacterial infection
PLoS Pathogens | 2017 Sep 20 | PubMed ID: 28931061 |
Read Article
SlZHD17 is involved in the control of chlorophyll and carotenoid metabolism in tomato fruit
Horticulture Research | 2021 Dec 01 | PubMed ID: 34848692 |
Read Article
Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single
-
cell level, et al. Development of an optimal protocol for molecular profiling of tumor cells in pleural effusions at single
-
cell level
Cancer Science | 2021 Apr 02 | PubMed ID: 33484069 |
Read Article
Prognostic impact of circulating tumor cells detected with the microfluidic “universal CTC
-
chip” for primary lung cancer, et al. Prognostic impact of circulating tumor cells detected with the microfluidic “universal CTC
-
chip” for primary lung cancer
Cancer Science | 2022 Jan 28 | PubMed ID: 34964211 |
Read Article
Integrated Study of Transcriptome-wide m6A Methylome Reveals Novel Insights Into the Character and Function of m6A Methylation During Yak Adipocyte Differentiation
Frontiers in Cell and Developmental Biolo… | 2021 Dec 03 | PubMed ID: 34926439 |
Read Article
Single-Circulating Tumor Cell Whole Genome Amplification to Unravel Cancer Heterogeneity and Actionable Biomarkers
International Journal of Molecular Scienc… | 2022 Jul 29 | PubMed ID: 35955517 |
Read Article
Isolation of TTF-1 Positive Circulating Tumor Cells for Single-Cell Sequencing by Using an Automatic Platform Based on Microfluidic Devices
International Journal of Molecular Scienc… | 2022 Dec 01 | PubMed ID: 36499466 |
Read Article
Genomic Analysis of Hematopoietic Stem Cell at the Single-Cell Level: Optimization of Cell Fixation and Whole Genome Amplification (WGA) Protocol
International Journal of Molecular Scienc… | 2020 Oct 06 | PubMed ID: 33036143 |
Read Article
Functional Analysis of the Active Site of a Metallo-β-Lactamase Proliferating in Japan
Antimicrobial Agents and Chemotherapy | 1999 Jul 06 | PubMed ID: 10952572 |
Read Article
A Rapid Method for Label-Free Enrichment of Rare Trophoblast Cells from Cervical Samples
Scientific Reports | 2019 Aug 20 | PubMed ID: 31431640 |
Read Article
Detection of circulating sarcoma tumor cells using a microfluidic chip-type cell sorter
Scientific Reports | 2019 Dec 27 | PubMed ID: 31882696 |
Read Article
Kinship analysis on single cells after whole genome amplification
Scientific Reports | 2020 Sep 04 | PubMed ID: 32887915 |
Read Article
First detection of TBE virus in ticks and sero-reactivity in goats in a non-endemic region in the southern part of Switzerland (Canton of Ticino).
Ticks and tick-borne diseases | 2019 Aug 15 | PubMed ID: 31047827 |
Read Article
Serological Investigation and Genetic Characteristics of Pseudorabies Virus in Hunan Province of China From 2016 to 2020
Frontiers in Veterinary Science | 2021 Dec 16 |
Read Article
A common variant of CNTNAP2 is associated with sub-threshold autistic traits and intellectual disability
PLoS ONE | 2021 Dec 13 | PubMed ID: 34898614 |
Read Article
Use of amplicon-based sequencing for testing fetal identity and monogenic traits with Single Circulating Trophoblast (SCT) as one form of cell-based NIPT
PLoS ONE | 2021 Apr 15 | PubMed ID: 33857205 |
Read Article
Haplotyping by linked-read sequencing (HLRS) of the genetic disease carriers for preimplantation genetic testing without a proband or relatives
BMC Medical Genomics | 2020 Aug 20 | PubMed ID: 32819358 |
Read Article
A novel method for noninvasive diagnosis of monogenic diseases from circulating fetal cells.
Prenatal diagnosis | 2021 Dec 10 | PubMed ID: 32673403 |
Read Article
Cell-based non-invasive prenatal diagnosis in a pregnancy at risk of cystic fibrosis.
Prenatal diagnosis | 2022 Jan 03 | PubMed ID: 33150588 |
Read Article
Reliable detection of subchromosomal deletions and duplications using cell
-
based noninvasive prenatal testing
Prenatal Diagnosis | 2018 Nov 19 | PubMed ID: 30357877 |
Read Article
Utility of Plasmodium falciparum DNA from rapid diagnostic test kits for molecular analysis and whole genome amplification
Malaria Journal | 2020 May 27 | PubMed ID: 32460780 |
Read Article
A Silicon-based Coral-like Nanostructured Microfluidics to Isolate Rare Cells in Human Circulation: Validation by SK-BR-3 Cancer Cell Line and Its Utility in Circulating Fetal Nucleated Red Blood Cells
Micromachines | 2019 Feb 17 | PubMed ID: 30781548 |
Read Article
Use of amplicon-based sequencing for testing fetal identity and monogenic traits with single circulating trophoblast (SCT) prenatal diagnosis
medRxiv | 2020 Jun 02 |
Read Article
Intercellular extrachromosomal DNA copy number heterogeneity drives cancer cell state diversity
bioRxiv | 2023 Jan 21 |
Read Article
Artificial intelligence-driven morphology-based enrichment of malignant cells from body fluid
bioRxiv | 2023 Jan 25 |
Read Article
>>
³í¹®
´õ
º¸±â (
¿¬°áµÇ´Â
ÆäÀÌÁö
ÇÏ´ÜÀ»
Âü°íÇØÁÖ¼¼¿ä)
Keyword : Sngle cell,´ÜÀϼ¼Æ÷,½Ì±Û¼¿,single cell analysis,single cell NGS,Ultra low input,WGA,Whole genome amplification,NGS,PGS,PGD,PGA,žƻêÀü°Ë»ç,»êÀü°Ë»ç,DNA-seq,CNV,SNV,SNP,Genotyping,array,variation,DNA variation
Whole Genome Amplification (WGA)
[±â¼ú°³¿ä] ThruPLEX & PicoPLEX
SNV, CNV °ËÃâ °¡´ÉÇÑ Whole genome amplificat..
PicoPLEX¢ç Single Cell WGA Kit v3
Whole genome amplification (WGA) from single..
PicoPLEX¢ç Single Cell WGA Kit