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NGS °ü·Ã
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Low Input DNA-Seq
> ThruPLEX¢ç Plasma-seq Kit
cell-free DNA library preparation for Illumina NGS platforms
ThruPLEX¢ç Plasma-seq Kit
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Clontech
R400679
ThruPLEX¢ç Plasma-Seq Kit
24ȸ
1,457,000¿ø
Product index
PGT-A
Embgenix¢â PGT-A Kit (..
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸..
mRNA-Seq (Single cell)
[New]Single cell mRNA-..
[New]Ultra low input m..
[New]High throughput, ..
Single cell mRNA-Seq K..
Ultra low input mRNA-S..
High throughput, Autom..
Ultra low input 3¡¯end..
Stranded RNA-seq
[New](+UMI) Pico i..
[New]Pico input strand..
[New]Single cell stran..
[New]Mid Input Strande..
[New]High Input Strand..
Total RNA-Seq Kit (FFP..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
Single cell Stranded R..
Mid Input Stranded RNA..
High Input Stranded RN..
Small RNA-Seq
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[New]UMI Àû¿ë Human TC..
[New]UMI Àû¿ë Human BC..
[New]UMI Àû¿ë Mouse TC..
[New]UMI Àû¿ë Mouse BC..
UMI Àû¿ë Human TCR pro..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
ChIP-Seq / Meth-Seq
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
Unique Dual Index Kits
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) Human TCR a/b..
(Legacy) PicoPLEX¢ç WG..
* º» Á¦Ç°Àº ±âÁ¸ ThruPLEX
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Plasma-seq Kit (Code R400490)ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°À̸ç, indexing primer kit°¡ Æ÷ÇԵǾîÀÖÁö ¾Ê½À´Ï´Ù.
Designed for cell-free DNA
: cell-free DNA ºÐ¼®¿¡ ÃÖÀûÈµÈ ÇÁ·ÎÅäÄÝ°ú ½Ã¾à
High performance NGS libraries
: high diversity with broad and reproducible GC coverage
Variable sample input
: <1 ng to 30 ng of cell-free DNA
Fast and simple workflow
: Æ©ºê À̵¿ ¾øÀÌ ´ÜÀÏ Æ©ºê¿¡¼ 3 step ÇÁ·ÎÅäÄÝ·Î NGS Library Á¦Á¶ °¡´É (library Á¦ÀÛ ½Ã°£: 2½Ã°£)
Research applications
: liquid biopsy, ctDNA¸¦ Æ÷ÇÔÇÑ ´Ù¾çÇÑ cell-free DNA »ùÇ÷κÎÅÍ ÀçÇö¼º°ú ¹Î°¨µµ ³ôÀº NGS ºÐ¼®
Automation-friendly
: ApolloTM Library Prep System(Takara Bio USA) Beckman
¢ç
FXP Workstation
Á¦Ç°¼³¸í
ThruPLEX
¢ç
Plasma-Seq Kit
´Â Ç÷Àå (Plasma) À¯·¡ÀÇ cell-free DNA·ÎºÎÅÍ
ThruPLEX 񃬣
À» ÀÌ¿ëÇÏ¿© Illumina
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NGS Library¸¦ Á¦ÀÛÇÏ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°Àº ±âÁ¸ Á¦Ç°¿¡ ºñÇÏ¿© End repair, ligation ½Ã¾à ¹× ¹ÝÀÀÁ¶°ÇÀ» ÃÖÀûÈÇÏ¿´À¸¸ç Plasma DNA·ÎºÎÅÍ ´Ù¾ç¼ºÀÌ º¸´Ù ³ô°í GC representationÀ» º¸À¯ÇÑ NGS Library¸¦ ±¸ÃàÇÒ ¼ö ÀÖ´Ù. Á¦ÀÛµÈ Library´Â CNV ºÐ¼®, Whole Genome Sequencing (WGS) »Ó¸¸ ¾Æ´Ï¶ó Custom panelÀ̳ª Agilent SureSelect, Roche NimbleGene SeqCap EZ¿Í °°Àº Target enrichment platform¿¡ Àû¿ë ¹× ºÐ¼®ÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº ±âÁ¸ ThruPLEX
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Plasma-Seq KitÀÇ ¾÷±×·¹À̵å Á¦Ç°À̸ç, Illumina index´Â Æ÷ÇÔÇÏ°í ÀÖÁö ¾Ê´Ù. ´ÙÄ«¶ó¹ÙÀÌ¿À¿¡¼ Á¦°øÇÏ´Â ¸ðµç DNA Index Kits¸¦ Àû¿ë °¡´ÉÇÏ´Ù (
DNA Index Kit ¹Ù·Î°¡±â
)
±×¸² 1. Illumina NGS workflow for cfDNA. Blood samples are collected, plasma is isolated, and cfDNA purified.
ThruPLEX Plasma-Seq library preparation follows and consists of three steps: repair, ligation and amplification. Indexed libraries are purified, quantified, and pooled prior to sequencing on Illumina NGS platforms.
±×¸² 2. ThruPLEX Plasma-Seq single-tube library preparation workflow.
The workflow consists of three simple steps that take place in the same PCR tube or well and eliminates the need to purify and transfer the sample material.
±×¸² 3. ThruPLEX Plasma-Seq technology.
A three-step, single-tube reaction that starts with fragmented dsDNA or isolated cfDNA. Stem-loop adapters are blunt-end ligated to the repaired input DNA. These molecules are extended, then amplified using a high-fidelity polymerase to yield an indexed Illumina NGS library.
Comparison data for Cell-free DNA-seq
KAPA Hyper Prep Kit, NEBNext Ultra DNA Library Prep Kit°ú ºñ±³ÇÏ¿©, ThruPLEX
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Plasma-Seq KitÀÌ º¸´Ù ¶Ù¾î³ ¼º´É°ú ÀçÇö¼º, GC Coverage¸¦ º¸À¯ÇÏ°í ÀÖÀ½À» ±Ô¸íÇÏ¿´´Ù (
Technical Note: An optimized solution for cell-free DNA library preparation
) (±×¸² 4, ±×¸²5).
±×¸² 4. Diverse, reproducible NGS libraries.
Libraries created with the ThruPLEX Plasma-Seq Kit yielded more unique molecules (Panel A), fewer duplicate reads (Panel B), and negligible unmapped reads (Panel C). Libraries were sequenced on an Illumina NextSeq 500 as a paired-end run with 17M to 25M reads per library. Duplication rate were calculated after down-sampling the data to 17M reads per library. Representative data from each sample is shown.
±×¸² 5. Reproducible, unbiased GC coverage.
The ThruPLEX Plasma-Seq Kit provided the most reproducible and unbiased GC coverage across the human genome, showing minimal variability across the nine plasma samples tested. Libraries were prepared from cfDNA isolated from an equivalent of 1ml of plasma sample and sequenced on an Illumina NextSeq 500. Four separate plasma samples were used to construct the NEBNext Ultra libraries.
Application protocol & Technical Note
Protocol: Plasma preparation for the ThruPLEX Plasma-Seq workflow
Technical Note: An optimized solution for cell-free DNA library preparation
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
- Template Preparation P Buffer
- Template Preparation P Enzyme
- Library Synthesis P Buffer
- Library Synthesis P Enzyme
- Library Amplification P Buffer
- Library Amplification Enzyme
- Nuclease-Free Water
Keyword :
Low Input DNA-Seq
All-purpose low input DNA library preparatio..
ThruPLEX¢ç DNA-seq Kit
cell-free DNA library preparation for Illumi..
ThruPLEX¢ç Plasma-seq Kit
PicoPLEX¢ç Single Cell DNA-Seq Kit